查询词典 Chain Reaction

In this study, a rab gene was obtained from Euplotes octocarinatus by polymerase chain reaction and RT-PCR.

本实验运用PCR、RT-PCR等技术，从八肋游仆虫中克隆到一种新的rab基因。

MethodsThe p73 gene ex- pression in 100 cases of AL including newly diagnosed and recrudescent or refractory ones and 20 normal controls was detected by relative quantitative reverse transcriptase polymerase chain reaction,and then the results were analyzed and compared by χ2-test,t-test and single agent variance. ResultsThe higher expression of p73 gene was found in 58% of AL cases.

采用RT-PCR方法检测100例初诊及复发或难治AL患者和20例正常对照者p73基因的表达，并采用χ2检验、t检验及单因素方差分析对结果进行统计学分析，以比较各组p73基因表达阳性率及表达强度差异。

METHODS: Female BALB/c mice were treated with CCK-8 and keyhole limpet haemocyanin, splenocytes were acquired and incubated in vitro with KLH restimulation, the productions of Th1 cytokines, interferon-γ, interleukin-2 and Th2 cytokines, IL-4, IL-5 in cell culture supernatants were detected by enzyme-linked immunosorbnent assay. The mRNA expressions of IFN-γ, IL-2, IL-4 and IL-5 in splenocytes were detected by reverse transcription-polymerase chain reaction. The levels of Th1 antibody IgG2a and Th2 antibodies IgG1 in serum were also detected by ELISA.

给予BALB/c小鼠钥孔戚血蓝蛋白免疫同时体内给予不同剂量的CCK-8，酶联免疫吸附试验检测其脾细胞培养上清中Th1型细胞因子γ-干扰素、白细胞介素-2（IL-2）和Th2型细胞因子白细胞介素-4（IL-4）、白细胞介素-5（IL-5）水平，逆转录聚合酶链式反应法检测脾细胞中IFN-γ、IL-2、IL-4、IL-5 mRNA表达；ELISA法检测血清中Th1型抗KLH抗体IgG2a和Th2型抗KLH抗体IgG1水平。

Methods Real-time quantitative retroverse polymerase chain reaction method was used for detecting RbAp46 expression levels in BM cells of 140 patients with acute leukemia,13 with chronic myelogenous leukemia in chronic phase, 7 with CML in blast crisis and 32 with non-leukemic disorders.

方法建立实时定量RTPCR方法，利用SYBRGreenⅠ染料检测140例急性白血病、13例慢性粒细胞白血病慢性期和7例CML急变期患者以及32例非白血病患者骨髓细胞RbAp46基因的表达水平。

The cDNA (BplPAL1) encoding phenylalanine ammonia-lyase were cloned from Betula platyphylla by reverse transcription polymerase chain reaction and 5' and 3' rapid amplification of cDNA ends, which contains an open reading frame (2322 bp) encoding 773 amino acids. The amino acid sequences contain two functional domains of PAL-HAL and PAL, and the sequence of enzyme active site, which has 60%~73% of consistency with other five plant species and the consistency with Rhizophora mangle is the highest (73.1%).

利用RT-PCR和RACE技术从白桦中克隆了编码苯丙氨酸解氨酶的cDNA，其2322bp的ORF编码773个氨基酸，其推导的氨基酸序列包含PAL-HAL和PAL2个功能域以及酶活性中心序列GTITASGDLVPLSYIA，该序列同其它5种植物的序列一致性为60%~73%，其中与美洲红树最高为73.1%。

Using real-time reerse transcriptase polymerase chain reaction, transforming growth factor- messenger ribonucleic acid expression was quantitatiely measured.

通过实时逆转录聚合酶链反应，定量测定转化生长因子信使核糖核酸的表达。

Methods The polymerase chain reaction-restriction fragment length polymorphism was used to analyze the gene polymorphism of ApoE in 25 high carotid artery IMT patients, 25 carotid artery sclerostenosis patients and 25 healthy age-matched controls.

应用聚合酶链反应-限制性片段长度多态性技术，对25例B超检测颈动脉内中膜增厚患者和25例颈动脉粥样硬化性狭窄患者及25例同期健康体检者的ApoE基因多态性进行分析，观察其多态性。

Methods The H gene was amplified and cloned from the passages of MeV23,MeV26,MeV27, MeV29,MeV32 and MeV37 of the attenuated strain of MeV vaccine strain S191 by reverse tran- scription potymerase chain reaction.

方法利用逆转录—聚合酶链反应方法扩增S191减毒株MeV23、MeV26、MeV27、MeV29、MeV32和MeV37代H基因，测序后与GenBank上U03663的序列进行比对分析。

Standard laboratory methods are based on virus isolation, serologic detection and Polymerase chain reaction.

目前猪瘟病毒的检测方法主要有病毒分离法、血清学检测、PCR等检测方法。

Naphthylamide heavy nitrogen spectrophotography was adopted to detect the content of nitric oxide in the serum and half quantitative reverse transcription polymerase chain reaction was used to detect eNOS gene expression in artery vessels.

采用萘胺重氮分光光度法测定血清NO含量，采用半定量RT PCR方法检测各组动物动脉壁匀浆中eNOS基因的表达，分析造模及各药物组血清NO含量及eNOS基因表达的变化。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。