查询词典 Chain Reaction

Methods Expression of the survivin mRNA was evaluated by reverse transcriptase polymerase chain reaction in 76 NSCLC tumor samples,20 benign phymatoid lesion and 21 adjacent normal lung tissue samples Immunohistochemical assay was to detect the expression of P53,c myc,k ras proteins.

研究细胞凋亡的调节机制对于弄清肿瘤包括肺癌的发病机制及新的防治方法具有重要意义。survivin是最近发现的一种凋亡抑制蛋白，在已检测的几种肿瘤组织中表达上调[1 3] ，在肺癌中的表达目前报道甚少，其与

The nucleotide sequences of the PAP gene and PAPⅡ gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPⅡ gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99. 8% were observed respectively. They both had one open reading frame . Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陆根为材料，提取其总RNA、反转录，用一对PAP基因的特异性引物和一对PAPⅡ的特异性引物，分别对反转录产物进行PCR扩增，将扩增的目的片段进行序列分析，结果扩增出来的PAP基因与已经报道的序列完全相同，扩增出来的PAPⅡ基因与已经报道的序列同源性也在99.8％，而且它们都各自编码一个开放阅读框架，可以说美洲商陆根中肯定存在PAP和PAPⅡ基因，并已经转录。

The nucleotide sequences of the PAP gene and PAPII gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPII gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99.8% were observed respectively. They both had one open reading frame. Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陆根为材料，提取其总RNA、反转录，用一对PAP基因的特异性引物和一对PAPⅡ的特异性引物，分别对反转录产物进行PCR扩增，将扩增的目的片段进行序列分析，结果扩增出来的PAP基因与已经报道的序列完全相同，扩增出来的PAPⅡ基因与已经报道的序列同源性也在99.8％，而且它们都各自编码一个开放阅读框架，可以说美洲商陆根中肯定存在PAP和PAPⅡ基因，并已经转录。

86 hypertensive patients with ischemic stroke . 85 hypertensive patients without ischemic stroke and 90 healthy blood donors were randomly enrolled in this trial . p-fibrinogen gene -455G/A polymorphy was analyzed from all subjects by polymerase chain reaction with restrictive enzyme HaeIII(PCR-RFLP method).

随机抽取住院高血压脑梗死病人86例，高血压非脑梗死病人85例，健康老年人90例，β-纤维蛋白原基因启动子区域-455G／A的多态性分析用多聚酶链反应加HaeⅢ内切酶检测。

METHODS A semi-quantitative reverse transcription polymerase chain reaction were performed to evaluate the expression of myostatin in the skeletal muscle of 5 Polymyositis patients and 4 healthy controls.

采用半定量RT-PCR方法检测5例多发性肌炎肌肉组织和配对非肌肉病对照者肌肉组织Myostatin mRNA表达及其与临床病理特征的关系。

Methods Forty - six prelingual deaf children and 30 children with normal comprehension were obtained, and the del（ GJB6 - D13S1830） in the Cx30 gene were screened by polymerase chain reaction in 2 groups.

方法收集46例散发的语前聋患儿及听力正常健康对照30例血标本，提取DNA后利用聚合酶链反应分析方法，筛查Cx30基因del（GJB6-D13S1830）突变。

The reverse transcription-polymerase chain reaction was used to seek the expressions of a specific fibroblast marker, prolyl 4-hydroxylase β, and a specific marker of epithelial cells, cytokeratin-4, in the cells.

采用逆转录多聚酶链反应检测hEFs表达成纤维细胞特异性标志（脯氨酰4-羟化酶β亚单位）和上皮细胞特异性标志（细胞角蛋白4）的情况，对该细胞进行鉴定。

Methods: HPV 16/18 infection was detected by nested-polymerase chain reaction, the p53 mutation was detected by direct sequencing, and the p53 and the HPV 16/18 E6 proteins were studied using immunohistochemistry on 129 pterygial specimens and 20 normal conjunctivas.

本研究利用巢叠式聚合脢锁反应分析自129例罹患翳状赘肉之翳状赘肉组织及20例正常无翳状赘肉的结膜组织检体中，是否有人类乳突病毒16及18型的感染，并利用直接定序的方式检测p53基因是否发生突变，此外亦以免疫组织化学染色法侦测p53蛋白与人类乳突病毒E6蛋白。

Methods: Semi-quantitive reverse transcriptase polymerase chain reaction was conducted to detect survivin mRNA and BCRP mRNA expression in puncturation tissue with breast cancer metastasis.

采用半定量逆转录-聚合酶链反应检测43例转移性乳腺癌转移灶中生存素和BCRP基因的表达水平，了解其与化疗疗效的关系。

Using primers of Pin1 and F3,molecular identification using polymerase chain reaction was made to detect Pythium and Fusarium in maize tissues.

利用Pin1和F3引物，对种植于不同接种物接种土壤处理、处于不同生长阶段的玉米不同部位组织进行了初步的分子检测。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。