查询词典 Chain Reaction

The CMS line 9704A, the restorer 8001 and F, population of 9704AX8001 containing 120 individual plants were used for molecular mapping of the restorer gene by bulked segregant analysis. 515 decamer primers with arbitrary sequences were chosen for polymerase chain reaction amplification. It is found that the polymorphism between the parents could be detected by 43. 8% of the primer and one RAPD marker OPL09-76I was tightly linked to the restorer gene of the restorer 8001 with the overcross value of 4.17% and genetic distance of 4. 18cM. The marker was repeatable and easy to score.

以辣椒CHS系9704A、恢复系8001及其组合9704A×8001F_2群体120株植株为材料，利用群分法法进行恢复基因定位，通过515个随机引物的PCR扩增分析，发现43.8％的引物在双亲间表现出多态性，找到一个与辣椒CMS恢复系8001中的恢复基因紧密连锁的分子标记OPL09_(-763)，该标记与8001的恢复基因的交换值为4.17％，遗传距离为4.18cM。

Methods: The mRNA expressions of VEGF, kinaseinsert domain-containing receptor and fms-like tyrosine kinase1 (flt-1) in the tubal pregnant decidual tissue, the normal oviductal tissue and the early pregnant human endometrial decidual tissue were detected and compared with semiquantitative reverse transcription polymerase chain reaction.

采用半定量RT-PCR方法，检测输卵管妊娠蜕膜组织中的VEGF、KDR、Flt-1 mRNA的表达，并与正常输卵管黏膜及正常宫内早孕子宫蜕膜组织比较。

Methods: By reverse transcriptase polymerase chain reaction and immunohisto-chemical assay, the expression of Surviving gene and bcl-2 protein were detected in 16 cases normal tissue of bladder, 12 cases papillate tumor and 59 cases transitional cell cancer of bladder.

采用逆转录PCR方法检测了16例正常膀胱组织、12例膀胱移行细胞乳头状瘤和59例膀胱TCC组织Survivin基因的表达，同时用免疫组化学方法检测以上组织中bcl-2蛋白的表达，并将结果进行相关分析。

Methods DNA was ext racted from paraffin-embedded tissue blocks of 44 angioimmunoblastic lymphaden o pathy patients and analyzed with polymerase chain reaction for IgH and TCRγ gene rearrangement. Immunohistochemistry staining was used to detect p53 protein expression.

方法运用聚合酶链反应技术检测44份AIL石蜡包埋组织标本中IgH及TCR γ基因重排情况，采用免疫组织化学ABC法染色技术检测AIL免疫表型及p53蛋白表达。

Methods Three kinds of special sequence primers for polymerase chain reaction with regard to the mutation styles (CGT、GTT、GAT) at codon 12 of K-ras were used to study the human pancreatic cancer cell line Patu 8988.The amplification products were studied with 12% polyacrylamine gel eletrophoresis to detect the style of point mutation.

针对K-ras基因第12位密码子点突变方式(CGT、GTT、GAT)设计顺序特异性引物，对人胰腺癌细胞株Patu 8988进行聚合酶链反应，扩增产物经12%非变性聚丙烯酰胺凝胶电泳后判断该细胞株有无K-ras基因点突变及其突变方式。

Polymerase chain reaction ; short tandem repeats ; genetic polymorphism ; Mongolia population ; VWA ; FGA ; PENTA E

聚合酶链反应；短串联重复序列；遗传多态性；内蒙古蒙古族群体； VWA ； FGA ； PENTAE

Methods Reverse transcription polymerase chain reaction technique was used to measure the expressions of MMP-8 mRNA in 22 cases of periapical granuloma,11 cases of periapical cyst and 10 cases of normal periapical tissues.

采用逆转录聚合酶链反应方法检测22例根尖肉芽肿、11例根尖囊肿及10例正常根尖周组织MMP-8 mRNA 的表达。

LIT was most commonly confused with malignant tumor, Crohn's disease, lymphoma and periappendicular abscess. There were 2 of 4 patients, who were performed operation. Most of our cases were not definitively diagnosed until the histopathologic examination after surgery and polymerase chain reaction.

大肠结核较易误诊为大肠肿瘤、克罗恩病、淋巴瘤和阑尾周围脓肿。4例剖腹手术者术中仍有2例(50%)被误诊为肿瘤和克罗恩病，即使活检或术后在病理学报告前仍有多数不能确诊。

Methods : The rotavirus RNA in the plasma samples and peripherial blood mononuclear cells of 30 patients with rotavirus diarrhea were measured by two kinds of reverse transcriptase polymerase chain reaction tests .

应用两种逆转录聚合酶链反应方法，对30例轮状病毒腹泻患儿进行外周血血浆和单个核细胞中的RV RNA检测，同时应用酶联免疫吸附法检测血浆IL-10水平，并与20例正常对照组进行比较。

The phosphine was quickly oxidized by triplet oxygen to phosphineoxide with a free radical chain reaction at relatively low temperature; while at high temperature, the phosphine react with singlet oxygen through a three member ring intermediate to form phosphineoxide and phosphinate as the product.

在相对较低温度时，膦化物与基态氧发生自由基链反应，膦化物很快被氧化为氧化膦；在相对较高的温度，膦化物与激发态氧直接作用，通过三元环状中间体而最终生成氧化膦和次磷酸酯。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。