查询词典 Chain Reaction

Ovarian neoplasma ; Multiple drug ; Resistance ; Gene expression ; Polymerase chain reaction

卵巢肿瘤；抗药性；多药；基因表达；聚合酶链反应

This was done using a high-performance liquid chromatography system equipped with an electrochemical detector, endonuclease nicking assay and reverse transcription polymerase chain reaction, respectively. RESULTS: H2O2 induced the formation of 8-oxo-dG in DNA at concentrations of 100 and 200 μmol/L.

结果：与对照组比较，100、200、300μmol/LH2O2浓度时均能使A549细胞DNA的8-oxo-dG水平增高（P.05或P.01）。200μmol/LH2O2作用12h后8-oxo-dG水平上升到峰值并在24h降至基线水平，8-oxo-dG水平在6~18h处理组显著高于对照组（P.05或P.01）。

Reverse transcription polymerase chain reaction was used to examine the deletions at P16 and FHIT in 52 patients with lung cancer and 9 patients with nonmalignant disease.

采用逆转录——聚合酶链反应方法，对52例肺癌患者和9例肺良性疾病患者的纤支镜活检标本中P16和FHIT基因缺失情况进行检测。

METHODS: Twentyfour patients with congenital heart defects were divided into 3 groups: mild pulmonary hypertension group(n=8), moderate or severe pulmonary hypertension group(n=9), and nonpulmonary hypertension group(n=7). NOS mRNA expression was detected in lung tissues with reverse transcriptase polymerase chain reaction.

将24例左向右分流先天性心脏病患者分为三组：轻度肺动脉高压8例，中重度肺动脉高压9例和无肺动脉高压7例，应用逆转录多聚酶链式反应检测患者肺组织中NOS mRNA的表达。

Methods Blood samples were obtained from 5 patients with AIDS, the C2~C3 region of HIV-1 envelope gene was amplified by nested polymerse chain reaction from plasma of each patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous and nonsynonymous substitutions as well as ds/dn ratios were calculated.

采用套式聚合酶链反应方法对5例艾滋病患者血浆HIV-1 env基因C2~V3区进行PCR扩增，产物经纯化后克隆至pMD-18T载体中，每例患者分别挑取5~8个克隆，共34株，完成阳性克隆的鉴定、抽提纯化和测序，将获得的DNA序列及核苷酸序列进行分析，并计算其核苷酸同源替换率与非同源替换率的比值。

Blood samples were obtained from 5 patients with AIDS, the C2~C3 region of HIV-1 envelope gene was amplified by nested polymerse chain reaction from plasma of each patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous and nonsynonymous substitutions as well as ds/dn ratios were calculated.

采用巢式PCR方法对5例艾滋病患者血浆HIV-1env基因C2~V3区进行PCR扩增，产物经纯化后克隆至pMD-18T载体中，每例患者分别挑取5~8个克隆，共34株，完成阳性克隆的鉴定、抽提纯化和测序，将获得的DNA序列及氨基酸序列进行分析，并计算其氨基酸同源替换率与非同源替换率的比值。

Infertility ; male ; Oligospermia ; Gene deletion ; Y chromosome ; Polymerase chain reaction

不育；男性；少精子症；基因缺失； Y染色体；聚合酶链反应

G banding karyotype analysis of peripheral lymphocytes in 217 patients with azoospermia or severe oligospermia were performed and the Y-chromosome AZFc region from seven cases with Y chromosome abnormality was amplified by polymerase chain reaction.

对217例无精和严重少精症患者外周血淋巴细胞染色体核型进行分析，并采用聚合酶链反应对7例Y染色体结构异常患者的AZFc区进行检测。

A polymerase chain reaction method was used to modify the growth hormone cDNA of Oncorhynchus tschawytscha.

采用聚合酶链反应方法改造鲑鱼生长激素cDNA。

METHOD: Fos ontogenic protein immunohistochemistry and double antigen immunohistochemistry method; semiquantitative reverse transcription-polymerase chain reaction method were used.

Fos 癌蛋白免疫组化LSAB 法结合双抗原标记法；半定量逆转录聚合酶链反应方法。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。