查询词典 Chain Reaction

Purpose To explore the value of single cell isolation from tissue slides and single cell polymerase chain reaction in the study of lymphy node germinal centers.

目的探讨从组织切片中进行单细胞分离和单细胞聚合酶链反应技术在淋巴结生发中心研究中的应用。

Methods Nested polymerase chain reaction was used to detect HPV B19-DNA on stock bone marrow smears from 84 children with ITP. Twenty-four cases of macrocytic anemia were chosen as controls.

应用巢式聚合酶链反应技术检测84例ITP患儿存档骨髓涂片标本中HPV B19-DNA，并以24例巨幼细胞性贫血患儿为对照。

With the method of reverse transcription-polymerase chain reaction and nest PCR, P80 gene of CSFV is cloned from the cells infected by the strain Shimen of csfv, and connected with eukaryotic expression vector pEGFP-C1. Then recombined eukaryotic expression plasmid pEGFP-P80 of P80 gene is achieved successfully. It builds the basis for expressing and gaining protein p80 in mammiferous cells.

利用反转录PCR和套式PCR技术，从猪瘟病毒Shimen株细胞毒中克隆出P80 基因，并进一步把P80 基因连接在真核表达载体pEGFP-C1 上，成功地构建出P80 基因重组真核表达质粒(pEGFP-P80)，为在哺乳动物细胞中表达并纯化出p80 蛋白奠定了基础。

RbcL gene was obtained by polymerase chain reaction and cloned into pDM19-T vector. The positive clone identified by PCR was sequenced. The nucleotide sequence was 1266 bp long, including an ORF of 1031bp, encoding a putative 343 amino acids. The deduced amino acid sequence had a high identity above 94.13% with that of tobacco, spinach, sweet potato, corn, Arabidopsis thaliana, Oryza sativa, Vitis vinifera, Marchantia polymorpha and Citrus paradise.

阳性克隆鉴定后进行测序，序列分析结果表明：该基因片断长为1266 bp，包括1031 bp的编码区序列，编码343个氨基酸；其编码区氨基酸与烟草、菠菜、玉米、甘薯、拟南芥、水稻、葡萄、地钱和葡萄柚等9个物种的同源性94.13%以上，并构建了它们间的亲缘关系树。

To establish Polymerase Chain Reaction method for detection Proteus mirabilis, so as to provide a reference method for sanitary inspection.

研究制定动物源性食品中奇异变形杆菌的PCR检测方法，为日常食品卫生监测提供快速检验的依据。

Objective To establish a multiplex polymerase chain reaction assay for simultaneous detection of Salmonella spp., Proteus mirabilis and Staphylococcus aureus.

目的 建立快速检测沙门菌、变形杆菌和金黄色葡萄球菌的多重聚合酶链反应方法。

Methods: The expression of Livin mRNA was measured in 91 AL and 17 chronic myelocytic leukemia patients by semi-quantity reverse transcription polymers chain reaction.

采用半定量逆转录-聚合酶链反应检测91例急性白血病和17例慢性粒细胞性白血病患者Livin mRNA的表达水平， 21名健康人的标本作正常对照。

Methods: The expression of Livin mRNA was measured in 91 AL and 17 chronic myelocytic leukemia patients by semi-quantity reverse transcription polymers chain reaction. 21 healthy adults were also measured as normal control.

采用半定量逆转录-聚合酶链反应检测91例急性白血病和17例慢性粒细胞性白血病患者Livin mRNA的表达水平， 21名健康人的标本作正常对照。

Methods The semiquantitative reverse transcription polymerase chain reaction was performed to evaluate the expression of myostatin in the skeletal muscle of 4 myotonic dystrophy patients and 4 healthy controls.

方法采用半定量RT-PCR检测4例强直性肌营养不良患者和4名非肌肉病对照者肌肉组织中myostatinmRNA的表达。

Methods The expression of CD87 was detected with flow cytometry in 54 AML patients and 20 acute lymphoblastic leukemia patients. The expressionsa of CD87 on U 937, HL-60 and K562 cells were also studied with FCM and reverse transcription-polymerase chain reaction during differentiation induced by phorbol myristate acetate in vitro.

应用流式细胞术检测了54例急性髓性白血病及20例急性淋巴细胞白血病患儿CD87的表达，还用FCM及逆转录-聚合酶链反应法检测了U937、HL-60、K562细胞在氟波酯作用下诱导分化过程中CD87表达的改变。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。