查询词典 Chain Reaction

In order to improve the viral detection method in shrimp, a more rapid and convenient multiplex reverse transcription-polymerase chain reaction was established in the present study. The new method was used for detection of shrimp viruses, including Yellow-head virus, white spot syndrome virus, Taura syndrome virus, hepatopancreatic parvovirus, infectious hypodermal and hematopoietic necrosis virus and monodon baculovirus.

为了提高对虾病毒检测的快速性和简便性，建立了一种可同时检测6种对虾病毒的RT-PCR方法，并利用该方法对来自广西沿海地区的130份病虾样品中的黄头病毒、白斑综合征病毒、桃拉病毒、肝胰腺坏死病毒、传染性皮下组织坏死病毒以及杆状病毒进行了检测。

In order to control the explosive epidemic disease of Penaeid shrimp in China, the one tube multiplex polymerase chain reaction were developed for detection of hypodermal and hematopoietic necrosis baculovirus.

根据对虾皮下和造血器官坏死杆状病毒运动HHNBV－XIA靶基因序列设计了4个多重PCR法用引物（P1、P2、P3、P4）。

In order to control the explosive epidemic disease of Penaeid shrimp in China, the one tube multiplex polymerase chain reaction were developed for detection of hypodermal and hematopoietic necrosis baculovirus.

根据对虾皮下和造血器官坏死杆状病毒HHNBV－XIA靶基因序列设计了4个多重PCR法用引物（P1、P2、P3、P4）。

A micronuclear version of the GARP gene was amplified by polymerase chain reaction. The macronuclear DNA molecule carrying the GARP gene is 460 bp long and shows the characteristics of macronuclear chromosomes of hypotrichous ciliates.

大核中该基因的染色体全长460 bp，基因两端具有下毛类纤毛虫大核特有的端粒序列(C4A4C4A4C4A4C4)，开放读框内含有一个TGA(88-99)密码子，在游仆虫中编码为半胱氨酸。

Methods Reverse transcription polymerase chain reaction method was adopted in detecting VEGF-CmRNA expression in 60 cases of breast infiltrative tubular cancer.

方法用逆转录聚合酶链反应法检测60例乳腺浸润性导管癌VEGF-C mRNA的表达情况，取15例乳腺纤维腺瘤标本作为对照。

MAIN OUTCOME MEASURES: At three and six weeks after injury, the infraspinatus were harvested to detect EGF, PDGF, TGF-β and BFGF by reverse transcription-polymerase chain reaction.

主要观察指标：术后3周，6周取岗下肌腱标本RT-PCR检测表皮生长因子、血小板源性生长因子、转化生长因子β、碱性成纤维细胞生长因子表达水平。

In nuclear style families, to study the genetic association and linkage relationship and to provide the genetic basis information for the further research. Methods: We investigated the genetic structure and relationship of 116 Keshan Disease patients and 65 local normal people in Shaanxi province with HLA-DRB1 locus, which is characterized by high polymorphism and inheritage stability, with polymerase chain reaction-sequence specific oligonuclotide probe.

本研究选用具有高度多态信息量及遗传稳定性的人类HLA-DRB1遗传位点，以陕西118名克山病人及当地65名正常对照人群作为研究对象，用聚合酶链反应—序列特异性寡核苷酸探针的方法对其遗传结构及其相互关系进行了分析研究，并在核心家系中采用基于单体型的相对风险及传递不平衡检验对其遗传指标进行了统计计算。

Methods: Matched normal and cancerous tis sues from 26 patients with primary gastric carcinoma and 30 patients with colorectal carc inoma were analysed by nested polymerase chain reaction and direct sequ encing of the products.

采 用RT-PCR及PCR产物直接测序法，检测了26例胃癌组织和30例大肠癌组织及其配对正常组织的FHIT基因。

The results showed:(1) MP polymerase chain reaction test showed positive results in the bronchoalveolar lavage fluid from all of the MP-infected rats (infectious group, n=4), while they were all negative in BALF from the control group (n=4,P＜0.05) and from those rats both infected with MP and, at the same time, treated with erythromycin (treating group n=4,P＜0.05). Bacterial cultures of the bronchial and lung tissue were negative in all three groups. Using transmission electron microscope the widened interalveolar septa with increased amount of collagen were found in the infectious group, while there were no obvious abnormalities in the other two groups.

结果显示：(1)感染组动物(n=4)支气管肺泡灌洗液肺炎支原体-PCR检测均为阳性，而对照组(n=4)和感染加红霉素治疗组动物(n=4)均为阴性(P＜0.05)；三组动物的支气管和肺组织常规细菌培养结果均为阴性；感染组动物透射电镜检查见肺泡间隔增宽，其中有较多胶原纤维堆积，其余两组则未见明显异常。

In order to analyze proteins which may interact with AIF, interceptive AIF gene (mitochondrial localization sequence, MLS, was deleted) which was amplified from the total mRNA extracted from hepatoma HepG2 cells by reverse transcriptase polymerase chain reaction, was inserted into pcDNA3.1 plasmid.

为了筛选可能与截断型AIF存在相互作用的蛋白质，探讨截断型AIF诱导细胞凋亡的机制，用逆转录PCR扩增剪切掉线粒体定位信号的人AIF基因片段并克隆入pcDNA3.1载体，导入肝癌HepG2细胞。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。