查询词典 Chain Reaction

By using new methods of isolating and purifying RNA of NDV, we improved the methods for detecting NDV based on Reverse transcriptase polymerase chain reaction and managed to detect velogenic NDV directly in 17 cloacal swabs specimens from different flocks including the 15 ones.

结果表明，生物学试验MDT和ICPI与ELISA阳性结果的强毒感染符合率为93.3％（14/15），而RT-PCR结果与ELISA阳性结果的强毒感染符合率为94.1％（16/17），而生物学试验加上RT-PCR结果与ELISA阳性结果的强毒感染总符合率为93.8％（30/32）。

Objectives The aim of this study is to establish rapid methods for detecting group A streptococcus, corynebacterium diphtheria and Legionella pneumophila Lp Respectively by polymerase chain reaction

目的：建立用于检测A组链球菌、白喉杆菌以及嗜肺军团菌的多聚合酶链反应方法。

The reverse transcription polymerase chain reaction RT-PCR and cDNA microarray were used to evaluate VEGF-C mRNA expression of T24 cells at around the best effect time and as the Crocin concentration reached the best.

用RT-PCR和基因芯片分别检测最佳作用时间和最佳药物浓度作用前后T24细胞VEGF-C的表达。

Methods The authors studied the expression of CyP gene from white blood cells in children with nephrotic syndrome by reverse transcription polymerase chain reaction.

采用逆转录聚合酶链反应测定28例肾病综合征患儿血白细胞中CyP mRNA的表达，并以正常儿童作对照。

In othor way, we use RT-PCR to observe the secretion of mRNA to compare with the balance of TH1/TH2 in spleen, so that we can see if we treat with cystamine can apparently raise the ratio of TH1 and also raise mRNA of CD25/ Foxp3, but reduce mRNA of CD40L. As a result of kidney which is the important secondary immunity organ discusses cystamin whether is the important control action or not to regulate in kidney then to be able to improve illness.

另外，透过了反转录聚合链锁反应(reverse transcription-polymerase chain reaction， RT-PCR)观察spleen中CD8/CD4 maker的mRNA表现量比较TH1/TH2 balance的倾向，发现经过胱胺处理后的确TH1相关的CD8 maker的mRNA比例量有大幅度上升，间接证明SLE倾向於TH2有被反转，而在Treg的特异性 maker方面CD25/ Foxp3之mRNA之比例也有大量上升，T cell的活性化maker CD40L也减少了mRNA的表现量，同时证实了SLE免疫倾向的反转，及发炎情况的减少及Treg cell的大量诱发。

Imatinib, a BCR-ABL tyrosine kinase inhibitor, is able to induce a complete cytogenetic response in more than 85% of patients with CML. However, the researchers note that patients with a complete cytogenic response tend to relapse when imatinib is discontinued, and less than 10% of patients achieve a molecular remission, defined by an undetectable residual disease using real-time quantitative–polymerase chain reaction.

Imatinib是一种BCR-ABL酪胺酸激酶抑制物，可以在85%以上的CML病患诱导完整的细胞生成反应；不过，研究者指出，完整细胞生成反应的病患倾向在停用imatinib时复发，不到10%的病患达到分子生物学上的缓解，其定义为以即时定量聚合酶连锁反应侦测不出残余疾病。

Imatinib, a BCR-ABL tyrosine kinase inhibitor, is able to induce a complete cytogenetic response in more than 85% of patients with CML. However, the researchers note that patients with a complete cytogenic response tend to relapse when imatinib is discontinued, and less than 10% of patients achieve a molecular remission, defined by an undetectable residual disease using real-time quantitative–polymerase chain reaction.

Imatinib是一种BCR-ABL酪胺酸激酶抑制物，可以在85%以上的CML病患诱导完整的细胞生成反应；不过，研究者指出，完整细胞生成反应的病患倾向在停用imatinib时復发，不到10%的病患达到分子生物学上的缓解，其定义为以即时定量聚合酶连锁反应侦测不出残餘疾病。

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人类T淋巴细胞白血病细胞系Jurkat作为研究对象，采用MTT法检测细胞增殖活性；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI双标法检测细胞凋亡；流式细胞术、Western Blot、RT-PCR检测鱼藤素作用前后，Jurkat细胞内核孔蛋白Nup88、Nup214与核磷蛋白(nucleophosmin， NPM)表达水平的变化；激光共聚焦显微技术观察上述核孔蛋白与核磷蛋白的亚细胞定位情况。

The ways that lead the team today will cause not merely the divisively between players and players or players and coaches, but also extend a chain reaction of managing problem, which effects the difficulty on the system and finally induces the result of the training unproductively if one keeps the old fashion ways of management.

现今的带队方式，要是再一成不变的依循传统，不仅球员与球员之间，或是球员与教练之间的互动会出问题，引申出来的管理问题会一连串的发生，造成制度运行上面的困难，而导致球队训练之成效不彰。

Methods Eight coding exons of ED1 gene of two patients with clinically confirmed X-linked hypohidrotic ectodermal dysplasia, their parents, and 100 unrelated population-matched control were amplified by polymerase chain reaction. The products were further analyzed by direct sequencing.

采用聚合酶链反应扩增该家系中两名临床诊断为X性连锁少汗性外胚叶发育不良的患者、患者父母以及100例健康对照者ED1基因的8个外显子，进行DNA直接测序。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。