查询词典 Chain Reaction

1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异；采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异；采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

At the end of treatment ,the differences in the behavior science were observed ; the change of the nasal mucosa secretion were inspected cross-reference after build model; IL-2,IL-4mRNA in the spleen were used to do revers transcriptive polymerase chain reaction.

用卵清蛋白致敏大鼠制成变应性鼻炎动物模型，辛芷鼻敏胶囊及阳性对照组药物鼻炎康灌胃给药，观察其行为学差异，造模前后分别取鼻粘膜分泌物涂片检查，用逆转录—多聚酶链反应对大鼠脾脏IL-2、IL-4mRNA进行半定量检测。

Cobra venom factor was given to prevent hyperacute rejection and FK506 was given to prevent cellular rejection. The xenograft recipients were killed and cardiac xenografts harvested 4, 8, 12, 16, 24 hours after transplantation. Histologic examination was used to demostrate the degree of rejection and coagulation while revers transcriptase-polymerase chain reaction was used to detect the expression of tissue factor mRNA, and the guinea pigs hearts, harvested but not transplanted, were studied as controls in all samples.

建立豚鼠－大鼠异种异位心脏移植动物模型，使用CVF抑制超急性排异反应，FK506抑制细胞免疫排斥反应，分别在移植术后4、8、12、16、24h切取移植心脏，通过病理切片观察排异反应和凝血强度，同时通过RT-PCR方法检测组织因子mRNA表达强度，未移植豚鼠心脏作对照。

Among these techniques, Northern blot, ribonuclease protection assay, real-time quantitative polymerase chain reaction, which are based on differentially screening and hybridization technology, have been applied most frequently.

本文概述了用于检测mRNA丰度的常规分析方法，即Northern印迹、RNase保护试验和实时定量PCR技术，特别是为对数量不多的基因进行表达情况的测定时提供了较为实用的方法。

Spotted fever group rickettsia\ \ Polymerase chain reaction

斑点热群立克次体；聚合酶链反应

Polymerase chain reaction was performed on lesion scrapings, aspirates, and filter paper impressions.

对病灶进行刮除，抽吸和滤纸印迹并进行聚合酶链反应。

Methods The nested polymerase chain reaction technique was used to detect the blood samples of patients with scrub typhus and compared with serologic detection of Rt.

采用巢式聚合酶链反应对11例确诊及10例疑诊恙虫病患者血液标本进行检测，并以血清IgG抗体间接免疫荧光试验对比分析。

Aim: To set up the methods of polymerase chain reaction-sequence specific primers for human ABO blood group, HAB secretor status and MN blood group genotyping and study the polymorphism of ABO blood group genes, HAB secretor status genes in part of Han population in Chinese and MN blood group genes in random blood donors and study the application of ABO genotyping in clinical transfusion and genetics.

目的：采用聚合酶链反应-序列特异性引物扩增基因定型方法，对ABO血型、HAB分泌型和MN血型系统基因定型，研究ABO血型、HAB分泌型和MN血型基因多态性分布特征及ABO血型基因定型在临床输血、遗传分析中的应用。

With western blot analysis and DNA polymerase chain reaction-single strand conformation polymorphism assay,protein product of Rb gene and mutation in exon 5～8 of P_(53) gene were examined in 17 cases of human testicular seminoma.

采用Western印迹和DNA聚合酶链反应－单股构造多态性分析法对17例人睾丸精原细胞瘤组织标本的抗癌基因Rb表达的蛋白产物和P_（53）基因外显子5～8的突变进行检测，发现有3例标本的Rb基因表达的蛋白产物缺失，有4例标本的P_（53）基因有点突变，对照的正常人睾丸组织和视网膜组织均呈Rb基因蛋白产物表达阳性，对照的正常人睾丸组织中未发现P_（53）基因突变。

The mRNA and protein level of bFGF was measured by semiquantitative reverse transcription polymerase chain reaction and immunohistochemical technique.

目的：检测碱性成纤维细胞生长因子在心房颤动患者心房组织中的基因表达，探讨其与心房纤维化的关系。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。