查询词典 Caryota

Binhai West Road, 9 kilometers (Zhenhai Yuehai Village to South Railway Station and the road back lots): The Guangdong, Hainan station near the coast, weather, soil water retention, poor performance of insurance manure, street trees to oil palm mainly due Caryota planting short,散尾葵, Areca triandra, red palm, coconut, such as bottles.

滨海西路9公里（镇海粤海村，铁路南站和公路回到地段）：在广东，广西，海南的海岸附近，天气，土壤水分，业绩不佳的保险粪肥，行道树石油棕榈油主要是由于种植短穗鱼尾葵，散尾葵，三药槟榔，红棕榈，椰子，如瓶子。

Objective To clone, express and characterize the panallergen profilin from the pollen of Caryota mitis Lour.

目的克隆并表达短穗鱼尾葵花粉中泛变应原肌动蛋白抑制蛋白。

Methods RT-PCR and RACE methods were applied to clone the full-length panallergen gene from Caryota mitis pollen.

方法利用RT-PCR结合RACE技术克隆短穗鱼尾葵花粉中泛变应原profilin的全长基因，并进行序列分析。

The profilin of Caryota mitis pollen was cloned and expressed successfully in E. coli BL21 (DE3), which will be used for further study on Caryota mitis pollen related allergy.

成功地克隆和表达了短穗鱼尾葵花粉profilin，为短穗鱼尾葵花粉过敏的诊断和免疫治疗奠定了基础。

Objective To characterize and purify the pollen allergens isolated from Carvota mitis. and Caryota ochlandra.

目的 对长穗鱼尾葵和短穗鱼尾葵花粉变应原蛋白进行分析、鉴定与纯化。

Methods RT-PCR and RACE methods were applied to clone the full-length panallergen gene from Caryota mitis pollen. The sequence of the insert was analyzed.

利用RT-PCR结合RACE技术克隆短穗鱼尾葵花粉中泛变应原profilin的全长基因，并进行序列分析。

Pollen allergens from Carvota mitts and Caryota ochlandra were purified and characterized. The result will provide a basis for the diagnosis and treatment of allergy to the pollen allergens from Carvota mitis and Caryota ochlandra.

对长穗和短穗鱼尾葵花粉变应原进行了初步的分离、鉴定和纯化，为临床鱼尾葵花粉变态反应疾病的诊断和治疗奠定了基础。

Two of these protein bands, with a molecular weight of 12 000 Mr and 14 000 Mr, showed immunoreactivity with IgE in the sera from patients with allergy to pollen of Caryota ochlandra. For the pollen extract of Carvota mitis, 30 protein bands were detected in SDS-PAGE, and three of these protein bands, with a molecular weight of 12000, 14000, and 26000 Mr, showed immunoreactivity with IgE in the sera from patients with pollen allergy of Carvota mitis. The 14000 Mr protein was the major allergen. Result from the ion-exchange chromatography indicated that the two pollen allergens (12 000、14000 Mr) from Caryota ochlandra were eluted in the second peak. The major pollen allergen of Carvota mitis was eluted in the forth and fifth peak.

结果 长穗鱼尾葵花粉有30余条蛋白带，其中主要条带有18条，12000和14000 Mr为长穗鱼尾葵花粉特异性变应原；短穗鱼尾葵花粉有30余条蛋白带，其中主要条带有9条，26000、1200和14000 Mr为短穗鱼尾葵花粉特异性变应原，其中14000 Mr为主要变应原；通过离子交换层析方法纯化出长穗鱼尾葵花粉相对分子质量为12000和14000的变应原主要分布在Ⅱ峰中，短穗鱼尾葵花粉相对分子质量为14000阳的主要变应原分布在Ⅴ峰和Ⅵ峰中。

The ORF of profilin of Caryota mitis pollen was amplified by RT-PCR using specific primers and cloned into the expression vector pET-28a. The recombinant Caryota mitis pollen profilin was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified by affinity chromatography using Ni(superscript 2+) coupled sepharose.

然后设计带有酶切位点的特异性引物，采用RT-PCR获得整个短穗鱼尾葵花粉profilin的开放阅读框，将其与pET28a载体连接并转化大肠杆菌BL21(DE3)进行诱导表达，通过Ni(上标 2+)亲和层析柱对重组蛋白进行纯化，采用Western-blot检测其IgE结合活性。

IgE reactivity of recombinant Caryota mitis pollen profilin was investigated by western blot. Results The Caryota mitis pollen profilin gene was cloned. The cloned fragment was 608bp in length, with an 396bp open reading frame coding for 131 amino acids.

结果 克隆获得了短穗鱼尾葵花粉profilin的全长基因，由608个碱基组成，开放阅读框为396个碱基，编码131个氨基酸。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。