查询词典 Calp

It is suggested that CaLP can induce the growth of aragonite through combined with the binding protein.

实验表明，CaLP可以与其结合蛋白共同诱导文石的产生。

Besides, the C-terminal tail of CaLP is very important in this process.

通过构建CaLP的缺失突变体证明CaLP末端的12个氨基酸在这个过程中发挥了重要作用。

By using the method of Western blot on fractions of nuclear and cytosol of primary cultured mantle cells and immunogold electron microscopy, we found out the completely different localization of these two proteins: CaM was localized both in cytoplasm and nuclear but CaLP was localized only in the cytoplasm.

通过对体外培养的珍珠贝外套膜上皮细胞核质分离后进行的Western blot分析和免疫电镜研究发现，CaM和CaLP的亚细胞定位是截然不同的：CaM分布于外套膜上皮细胞的细胞核与细胞质中，而CaLP却仅分布于外套膜上皮细胞的细胞质中。

This research is based on our previous successful cloning of a completely novel calcium metabolism related protein—calmodulin-like protein from pearl oyster and calmodulin. The main purpose of this research is to find out the function of CaLP on the cell level and compare functions of these two proteins.

为进一步揭示贝类生物矿化机理，本研究以目前我国用于生产海水珍珠的主要贝类合浦珠母贝为研究材料，以本实验室已克隆出的新蛋白钙调素类似蛋白(calmodulin-like protein，CaLP)为研究对象，对CaLP在细胞水平上的功能进行了进一步的研究，并将其与本实验室已克隆出来的另一钙代谢相关蛋白钙调素(calmodulin，CaM)进行了比较。

The immunochemical study showed that CaLP may locate around the prisms and in the organic layer sandwiched between the prismatic layer and the nacre.

贝壳原位免疫实验显示，CaLP可能分布在贝壳棱柱层的棱柱周围以及棱柱层和珍珠层之间的有机夹层中。

During a special biomineralization process—shell regeneration, we found that both CaLP exhibit special functions in shell related secretory vesicles; during the differentiation of osteoblast MC3T3-E1, CaLP exhibite significant effect on its differentiation and biomineralization.

在对切壳修复这一特殊的生物矿化过程的研究中发现CaLP积极参与了新生贝壳的形成；在前成骨细胞MC3T3-E1向成骨细胞分化的过程中，CaLP能够促进成骨细胞的分化和矿化过程。

CaLP's phosphorylation state analysis of different localization showed that when CaLP is overexpressed alone, it is in state of phosphorylation and stays in the cytoplasm; when CaLP is co-transfected with p21, p21 overexpression causes its unphosphorylation and translocated from cytoplasm to nucleus.

对定位于不同部位的CaLP的磷酸化状态的分析表明：当在细胞中单独表达CaLP时，它以磷酸化形式存在，且磷酸化的位点发生在Y139上，CaLP因此定位于细胞质中；当共表达CaLP与p21时，p21的过表达引起CaLP发生去磷酸化，CaLP由细胞质穿梭入细胞核并与p21发生直接相互作用。

Another interesting phenomenon is that when CaLP and p21 are co-transfected, the expression of p21 can stimulate CaLP translocate from cytoplasm to nucleus. By using methods of bioinformatics, point mutation and flow cytometry, we confirmed the importance of Y139 in the nuclear-cytoplasmic trafficking of CaLP.

激光共聚焦实验还发现当p21过表达时能够引起CaLP从细胞质向细胞核的转移，通过生物信息学方法，免疫共沉淀实验和流式细胞分析表明，CaLP的139位酪氨酸残基存在着较高的磷酸化潜力且在CaLP的核质穿梭和蛋白相互作用中起到了重要的作用。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。