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CCK相关的网络例句
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However, the high molecular weight and metabolic instability of CCK has precluded its use as an anorectic agent.

但栀子的主要成分栀子甙及几个含量较高高的组方不显示拟胆囊收缩素作用。

All the CCK--8 effects could not be blocked by atropine or indomethacin.

这些作用均不能被阿托品和消炎痛所阻断。

Objective To observe the effect of cholecystokinin on energy metabolism of obesity rats after acupuncture.

目的 观察肥胖大鼠针刺后胆囊收缩素(cholecystokinin,CCK)对其能量代谢的影响。

It has been well documented that cholecystokinin, a kind of brain-gut peptide, is involved in many physiological and pathophysiological processes.

胆囊收缩素(cholecystokinin,CCK)是一种脑—肠肽,参与了多种生理及病理生理过程。

Cholecystokinin , firstly identified as a gastrointestinal peptide, is wildly distributed throughout the centr...

CCK是一类典型的脑肠肽,在中枢神经系统以神经递质或调质的形式,通过其受体发挥重要作用。

After 1% Nembutal (35mg/kg) was injected anaesthesia, we applied OHBA SIKI spinal cord beat equipment and finished contusion model (beat intensity is 25 Vgram-cm force, gcf) with animal of the latter three groups, only laminectomy of T8-9 were finished on the rats of control group.

除正常对照组外,其余三组大鼠使用OHBA SIKI打击器(Tokyo,Japan)按Gruner改良法作成T9脊髓背侧损伤模型,打击强度为25势能克厘米(Vgram-cm force, gcf),并于打击前向生理盐水对照组和CCK-8治疗组大鼠自尾静脉分别注入生理盐水及胆囊收缩素八肽(CCK-8 40ug/kg)0.2ml。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池,将lipid A包被于非衍生样品池,分别建立以PGN、CpG DNA及lipid A为靶点的技术平台,对114种抗炎中药水提物进行筛选、评价其活性物质含量,并评估出针对上述三种病原分子均具有较高结合活性的中药;(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术,从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分;在体外实验中,测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力;MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响;ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用;在体内实验中,采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌,建立细菌脓毒症小鼠模型,观察活性组分对脓毒症模型小鼠的保护作用。

The morphology of the cells was observed by inverted phase contrast microscope and cell proliferation determined by CCK-8 assay.

用倒置相差显微镜观察细胞形态和生长情况,细胞增值率通过CCK-8检测。

Patients with hepatic cirrhosis and 10 healthy controls were studied. Plasma VIP and CCK were measured with radioimmunoassay and gallbladder emptying function was measured by ultrosonography with fat meal test. The plasma VIP and CCK of patients were all higher than that of controls.

用放免法测定肝硬化患者及对照者血浆血管活性肠肽和胆囊收缩素含量;用B超测定餐前、餐后胆囊容积及排空率。

CoCl2 (a chemical hypoxia-mimetic agent) was used to establish the chemical hypoxia-induced PC12 cell injuries model. Sodium hydrosulfide was used as a H2S donor. The viability of PC12 cells was measured by CCK-8 assay. The percentage of apoptotic cells was assessed by propidium iodide stain flow cytometry. The morphological change of apoptotic cells was tested by using the chromatin dye Hoechst 33258. The mitochondrial membrane potential was analyzed by rhodamine 123 staining and photofluorography. The level of reactive oxygen species in PC12 cells was measured by DCFH-DA staining and photofluorography.

应用化学性缺氧模拟剂CoCl2在PC12细胞建立化学性缺氧损伤模型;以硫氢化钠作为H2S的供体;应用CCK-8比色法检测细胞存活率;碘化丙啶染色流式细胞技术检测细胞凋亡率;Hoechst33258染色检测细胞凋亡的形态学变化;罗丹明123(Rh123)染色荧光显微镜照相检测细胞线粒体膜电位;2',7'-二氯荧光黄双乙酸盐染色荧光显微镜照相检测细胞内的活性氧水平。

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