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Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml,分离单个核细胞体外诱导培养DC,并将实验组和对照组细胞各分为三组,A组为未处理组,B组为冻融组,C组为RNA转染组。A组DC在培养5d后,每孔加入TNF-α200U/ml;B组DC培养第5d后,每孔中加入癌细胞蛋白抗原,使DC/肿瘤细胞=1:1,继续培养4h后,每孔加入TNF-α200U/ml;C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC:RNA=1×105/ml: 5ug,按照invitrogen DMRIE-C试剂说明书进行操作),转染结束后用完全培基取代转染剂,每孔加入TNF-α200U/ml。

This paper makes comparison of Visual C++ and C++ Builder from aspects of the application framework, applicability, transplantability , and application prospect, etc.

C++Builder和Visual C++是目前比较流行的面向对象的程序设计语言,从应用程序框架、易用性、可移植性、应用前景等方面对C++Builder和Visual C++进行了对比分析。

We can infer from the each group of mice''s training of swimming in cold water that group CⅠand Ccan adapt to the stimulation of coldness, while group CⅢ shows unwellness then adaptation and unwellness at last.

从各训练组小鼠进行冷水游泳训练情况可以推测出,CⅠ组,CⅡ组表现出对寒冷刺激的适应,而CⅢ组则表现出由不适应到适应再到不适应的特点。

In the treatments with sterilization and without sterilization.the biodegrading frequency of C.test、 Pktac- A8、 C.test Pa、 C.test Pb was increasing with the temperature between 4°C and 30°C.

在四种菌株降解胆固醇的比较中,C.test Pa的降解率达到最大,且与其它三种菌株之间差异达到极显著水平。

The results indicates, with the same level of other parameters, the TN removal can reach 80.0% and 70.9% with the control of dissolved oxygen under the levels of 0.3-0.6mg/l and 0.6-0.9mg/l, respectively; the TN removal upgrades from 80%, 83% to 84.1% with the decrease of sludge load in the system from 0.1, 0.07, to 0.05gCOD/gMLSS·d; the nitrogen removal only reaches 58% when C/N is 4, but can reach more than 80% when C/N is at or over 7, which indicates that the TN removal increases when higher C/N ratio; when HRT increases from 12h, 18h to 24h, the TN removal also increases from 78.6%, 82.0% to 83.0%; the TN removal average in the system increases from 75.6%, 78.5% to 83.0% with the increase of sludge age from 18d, 20d to 24d; the low oxygen conditions in the modified Orbal oxidation ditch model do not decrease the effect of COD removal, and the dissolved oxygen gradient with the distribution of 0-2-1mg/l in the system ensures the COD removal reaching the range of 80%-86%; and the phosphate removal, however, is not good enough, which varies from 40% to 50%, even lower than 35% in occasional situations, and the phosphate concentration in the outflow varies in the range of 1.28-2.38mg/l, which dissatisfies the discharge standard with the level of 1.0mg/l.

试验结果表明,在其它参数相同的情况下,将外沟道的溶解氧分别控制在0.3~0.6mg/l 和0.6~0.9mg/l 两个水平,外沟道中TN 去除率分别为80.0%和70.9%;系统中污泥负荷分别为0.1gCOD/gMLSS·d、0.07 gCOD/gMLSS·d、0.05 gCOD/gMLSS·d时,随着污泥负荷的降低,TN 的去除率却是上升的,分别为80%、83%、84.1%;本试验中,当C/N 为4时,仅能获得58%的脱氮率,当C/N 为7 或更高时脱氮率能达到80%以上,进水C/N 比值越高,总氮的去除率越高;在本试验中采取的水力停留分别为12h、18h、24h 时,外沟道中的总氮去除率随水力停留时间的增加而增加,分别为78.6%、82.0%和83.0%;当系统泥龄分别为18d、20d、24d 时,系统平均TN 去除率分别为75.6%、78.5%、83.0%,随着泥龄的增大TN 的去除率增加;改良型奥贝尔模型的低氧条件并没有降低COD 的去除效果,系统0-2-1mg/l的溶解氧梯度分布使得COD 去除率一般都在80%~86%之间;本试验中磷的去除效果不好,去除率基本在40%~50%之间,有的甚至低于35%,出水磷的浓度在1.28~2.38mg/l 之间,低于1.0mg/l 的出水排放标准。

Though the primary stimulator of 1H is not clear, it is related to physical, cell and body fluid IH is a chronic process involving multiple faction injury and blood flowing abnormal are revamped as the main reason, the proliferation level depend on the length and width of the injure of vessel large amount research illustrate injury cause many growth factor infolded in IH, for example: template derived growth factor, fibroblast growth factor, transmission growth factor , endothelin (ET-1) et al, some early responsive gene such as c-fos、c-jun c-myc also take part in SMC proliferation after vessel injury.

虽然IH的确切起始刺激因子并未阐明,但已知与物理、细胞和体液因子有关。IH是一个多因素参与的慢性过程,损伤和血液动力学异常一真被认为是IH的主要因素。内膜增殖的程度有赖于血管损伤的长度和深度。近年来大量的实验研究结果表明,损伤等刺激引起的众多的生长因子样物质在IH中起关键作用。如血小板源性生长因子、碱性纤维母细胞生长因子、转化生长因子β、内皮素-1(ET-1)等诸如C-fos、C-jun、C-myc等早期应答基因也参与了血管损伤后SMC的增殖。

The active extract from malted is vitamin C stabilized with glucose. The benefit of combination of vitamin C and glucose is to make vitamin C be applied in cosmetic more easily and effectively.

麦牙活性提取物是用葡萄糖来稳定化的维生素C,这种维生素C与葡萄糖结合带来的好处在于能使维生素C更方便和有效地应用于化妆品中。

Are the main products are: KD-260, A, B, C Zhenshi high-speed packaging machine; KD-350, A, B, C Zhenshi high-speed packaging machine; KD-450, A, B, C-type high-speed Zhenshi Packaging Machine (reprovisioning:-nitrogen, hot Dama Ji, jack devices).

现主要产品有:KD-260、A、B、C型高速枕式包装机;KD-350、A、B、C型高速枕式包装机;KD-450、A、B、C型高速枕式包装机;(另配:冲氮气、热打码机、插口装置)。

Alternate connective has a few kinds of cases, I say to basically leave two kinds of commonly used way here: Home page is specification of station of A B C falls: If A C station is two my different sites, b station is the station of the other side, I and the other side change home page connective moment, I use the connection that B of the other side of the put away on A station stands,(This join also is one changes, because a lot of person misunderstood across,join is not one changes,) it is B of the other side next the link that needs to put my C to stand only on the station went, need not go putting the join that my A stands, also be one changes a join, this is the resource station that we provide many bits just.

交叉连接的有几种情况,这里我说下主要下常用的两种方式:首页是 A B C 站说明下:如果 A C 站是我的两个不同的站点,B站是对方的站,首页我和对方换连接的时候,我用A站上放好对方B站的连接,(这个连接也是一个换一个的,因为好多人都误会了交叉连接不是一个换一个,)然后是对方B站上只需要放我C站的连接就行了,不用去放我A站的连接了,也是一个换一个连接,这个是我们提供的资源站多点而已。

When we used those two multivariate statistic analytical techniques to study the spatio-temporal variation laws of benthic community structure at Shenzhen Bay, the results indicated that: 1 There was not obverse difference in intertidal benthic community structure between Futian, Shenzhen and MaPi, HongKong in Shenzhen Bay in Spring, 2000. However, there was continual gradient change in community structure from outer bay to inner bay. 2 There was obvious deference with the respect of benthic community structure between station C and stations Al, A2, A3 in transect A and station B in transect CBA in Shenzhen Bay. There being no benthos at station C in Summary, 2000, it indicated that station C had been polluted very heavily. 3 Deferent seasons and deferent tide sites are the most two important factors which play influence on benthic community structure at stations Al, A2, A3 and B3 in Shenzhen Bay.

用这两种多变量方法研究深圳湾底栖动物群落结构时空差异,结果表明(1)2000年春季深圳湾底栖动物群落结构组成在深圳福田一侧与香港米埔一侧并没有明显的差异,而是沿湾外向湾内呈现连续的梯度变化;(2)在深圳福田一侧的C断面的底栖动物群落结构与A、B断面有明显的差别,2000年夏季在C断面没有采到任何底栖动物,表明C断面已经遭受了严重的污染;(3)季节和潮区是影响深圳湾A1、A2、A3、B3站位底栖动物群落结构组成时空差异的两个最重要的因素。

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I didn't watch TV last night, because it .

昨晚我没有看电视,因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来,在北京的很多小区里,电梯液晶电视被撤了下来。

I'm running my simile to an extreme.

我比喻得过头了。