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In this study,Amplified fragment length polymorphism analysis was carried out by using 23 pairs of EcoRI-NN/MseI-NNN selective primers in amplification of the DNAs from reciprocal F_1 hybrids of Cucumis hystrix Chakr.and C.

为了研究细胞质背景对基因组序列丢失的影响，利用23对Eco RI-NN/Mse I-NNN选择性引物对Cucumishystrix与栽培黄瓜C.sativus的正反交F_1、异源双二倍体及亲本DNA进行AFLP分析。

Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出，等位基因平均值、观察杂合度、Fis值、Fit值皆较高，表明非洲菊等位基因较丰富，杂合基因偏多，且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium；PP8、na、Ⅰ、h、Gi及Fst皆表明，ISSR检测遗传多样性的能力最强，其次是RAPD，等位酶最低；根据遗传距离进行聚类，三种方法都把70个品种分成A、B、C、D、E五个大组，每一组中除少数品种发生位移外，大部份品种分类结果相似，且与形态分析结果有相似性，但在每一组中，品种间的聚类差别较大，表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同；Mantel检测表明，三种标记的分析结果有显著相关性，表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异；非洲菊为组培苗，三种标记的检测结果皆表明，混合样品可以作为个体样品的代表，对整个居群的遗传多样性进行评价；这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

The result shows that PtRuMoO_3/C shows the best catalytic activity towards methanol and phosphomolybdic acid modified PtRuMoO_3/C electrode can be considered as the best anodic electrode for DMFC.

分别采用磷钼酸和磷钨酸对三种催化剂得到的催化电极进行了修饰，并用循环伏安曲线和不同电位下的恒电位I-t曲线测试了它们的性能。

Coumarinyloxy)-6-methoxyl-7-hydroxycoumarin,β-sitosterol, 3-stigmast-5-en-3- 3 -D-Glucopyranoside . 3-(7-coumarinyloxy)-8-(7-hydroxycoumarin-8-yl)-7-hydroxycoumarin rhamnoside and Sucrose. Compound I , V, IX and XI were isolated from barks and stems of this plant for the first time and compound VI and VI were isolated from flowers of this plant for the first time, too.

共鉴定了其中11个化合物，根据理化性质和波谱数据鉴定确定为：丁香甙，结香甙C(Edgeworoside C Ⅱ)，7-羟基香豆素(7-hydroxycoumarin Ⅲ)，5，7-二甲氧基香豆素(5，7-dimethoxycoumarin Ⅳ)，卫矛醇，异吲哚-1，3-二酮1H-iso-Indole-1，3(2H-dione Ⅵ，西瑞香素(Daphnoretin Ⅶ)，β-谷甾醇(β-sitosterol Ⅷ)，胡萝卜甙(Daucosterol Ⅸ)，结香甙A(Edgeworoside A Ⅹ)和蔗糖。

The frequency of CMV seronegativity showed a stepwise decrease with the increase of donor age. In addition, the prevalence of HBsAg, antibodies to hepatitis C virus, antibodies to human immunodeficiency viruses type 1 and 2 (anti-HIV 1+2) and antibodies to human T-cell lymphotropic viruses type I and II (anti-HTLV 1/11) were compared between CMV seropositive and seronegative groups. Our results showed that there was no significant difference in seroprevalence of these markers between CMV seropositive and seronegative groups.

为了更进一步了解巨细胞病毒抗体和其它血液筛检项目之相关性，吾人在同批检体中比较巨细胞病毒抗体阳性者和阴性者其 B 型肝炎表面抗原、C型肝炎抗礼、第一型暨第二型人类免疫缺乏病毒抗体和第一型暨第二型人类嗜 T 淋巴球病毒抗体之阳性率，结果发现这些阳性率在两者间无有意义之差异。

The Bosnian mission was using twelve C-17s, and I had to fly one into Tuzla; the regular Air Force One, a Boeing 747, was too big.

波斯尼亚行动需要动用12架 C-17，我不得不乘其中一架飞到图兹拉；常规的&空军一号&波音747太大了。

First of all,it discussis develop background and synopsis of this system;then,it discussis three technologies of developing thods,namely,it involves in tway of SDLC,the way of Prorotype,the way of OO,and the way of th system;it elaborately expound technologies and appviation of Systems Analysis and System Design,especialy the commu between ement and Students by meas of C/S(Client/Server);it also discussis the question and the knowledge during develop this system;during develop this system, I understand the thinking and method of develop MIS.

全文共分为绪论、开发方法的选择、系统分析、系统设计、系统实施、结束语等六章。首先论述了该系统的开发背景和系统的简单介绍；然后，讨论了结构化生命周期法、原型法和面向对象这三种开发方法的技术，根据需要选择合适的开发方法；结合本系统详细阐述了系统分析、系统设计的技术及应用，尤其在机房管理人员和上机人员的交流方面采用了C/S结构进行解决；此文还论述了在开发过程中遇到的问题及学到的东西。

This is the first report of pectenolone and pectenoxanthin, two types of carotenoid, in the muscle of the Yesso scallop.3 Roles of the carotenoids composition and accumulation in Yesso scallopThe method for detecting carotenoids using reverse phase high performance liquid chromatography was developed. The conditions of RP-HPLC analysis were an analytical scale (200×4.6 mm, inner diameter 5μm) Eclipse XDB-C18 column of Agilent corporation; 10μl injection volume; 25°C column temperature; acetonitrile, methanol and methylene dichloride mobile phase (40:56:4, V/VV) with 0.1% BHT; 1.0 ml per minute flow rate; 450 nm UV-VIS detector; 8 minutes elution.The carotenoids from orange muscle of Yesso scallop of 7, 10, 12, 15, 18, 22 months old was detected using the method refered above.

通过柱层析、高效液相等手段对橘红色闭壳肌中的主要类胡萝卜素进行了提取分离，分离提纯得到两种类胡萝卜素，并通过MS、~1H-NMR和~(13)C-NMR对其进行结构鉴定，结果表明(3S，3\'R)-3，3\'-dihydroxy-7\'，8\'-didehydro-β，β-Caroten-4-one和(3S，3\'S)-7，7\'，8，8\'-tetradehydro-β，β-Carotene-3，3\'-diol，俗称pectenolone和pectenoxanthin是橘红色闭壳肌中的主要类胡萝卜素，这是国内外首次对虾夷扇贝闭壳肌中的类胡萝卜素进行提纯及结构鉴定。3虾夷扇贝类胡萝卜素的种类组成和累积变化规律建立了橘红色闭壳肌中类胡萝卜素的HPLC分析定量检测技术：色普柱为Agilent公司的Eclipse XDB-C18柱200mm×4.6mm，i.d.5μm进样体积，5μl ；流速1ml/min；检测波长450nm；柱温25℃；流动相为乙腈：甲醇：二氯甲烷(V/V/V=40：56：4并加入0.1%的BHT；分离时间为8min。

Three kinds of intra-laboratory quality control tests of RIA for serum insulin and C-peptide,i.e.monitoring by quality control serum,by two-sample Youden plotting and by standard curve parameter are described in the present paper.

本文介绍了胰岛素、C肽RIA检测中的三种室内质控方法，即质控血清监测法，两样本Youden图监测法和标准曲线参数监测法，并对其结果做了初步分析。

Cfff3ad00Battalion|r : C-130s are commencing Phase I airdrops.

|cfff3ad00指挥部|r ： C-130 正在进行空投。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。