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Rat in Group B was set an air bladder which was empty and not perfused water. Water of 0.6 ml, 0.8 ml, 1.0 ml, 1.2 ml, 1.4 ml and 1.5 ml was perfused respectively into the air bladders which were set in the colon of rats in group C, D, E, F, G and H. Then the changes of ethology of the rats were recorded in interval of an hour.

应用疼痛行为学的观察方法，观察1h各组动物的行为学改变，并采用免疫荧光化学的方法，观察大鼠DCN中神经元被激活的标志c-fos及胶质细胞的胶质原纤维酸性蛋白(glial fibrillary acidic protein， GFAP)和小胶质细胞特异性补体OX42的表达情况，空白对照组是在疼痛高峰点(1.2 ml)处死取材。

The preferred method is to autoclave for 1 h at 121°C; disposables should be autoclaved or incinerated.

最好的方法是在 121°C 高压蒸气灭菌1小时。一次性用品应高压蒸气灭菌或焚烧。

R c h r i s t 50 at yahoo dot c o m put all together you can reach my heart I am slso willing to relocate to you, or you can come here with me., write me I am open to discuss to bring love into my life. I would love to find someone I could share a life in China

我也愿意搬到你那里住，或者你到我这里来，我想找个人一起分享中国的生活。

With treatment of 2 dyne/cm2 of shear stress, c-myc mRNA reached a peak at 1.5 h, and then slightly decreased.

在未受剪切力作用的RPE细胞中，c-myc mRNA表达水平较低。

The efficient first asymmetric syntheses of-6β-hydroxy-7αH-eudesm-4-en-3-one, and its C-6 epimer were achieved starting from-dihydrocarvone, whichprovide a novel and efficient synthetic method to 6-oxgenated eudesmanes.

以-二氢香芹酮为原料，经五步反应，首次完成了6β-羟基-4(5)-烯-3-羰基桉烷及其6位差向异构体的立体选择合成，并为C-6位含氧官能团取代桉烷类化合物的合成提供了一种简便有效的方法。

Incubate at 37 1 °C for 24 h.

1 °C培养24小时。

Incubate the pre-enrichment broth BPW at 37 1° C for at least 16 but not more than 20 h.

在37 1° C培养BPW富增液，至少16小时，但不能超过20小时。

Incubate the inverted dishes at 37 1 °C for 22 - 24 h.

将盘翻转，在37 1 °C培养22-24小时。

The optimal medium components were: sodium alginate 3 g/L,(NH4)2SO4 3 g/L, NaCl 20 g/L, KH2PO4 0.1 g/L, CaCl2 0.1 g/L; The optimal culture conditions were: 25 ml medium in 250 mL Erlenmeyer flask, inoculum's volume 3%, shaking speed of 150 r/min, initial pH 7.5, at 28°C for 24 h.

该菌株产酶的最佳培养基组成为：褐藻胶3 g/L、(NH4)2SO4 3 g/L、NaCl 20 g/L、KH2PO4 0.1 g/L、CaCl2 0.1 g/L；最佳培养条件为：250 mL三角烧瓶中装液量25 mL、接种量3%、摇瓶转速150 r/min、pH7.5、培养温度为28℃、培养时间为24 h。

L Bouyer, Christian Humanism; Q Breen, John Calvin: A Study in French Humanism; H Kung, On Being a Christian; J Maritain, True Humanism; JI Packer, Knowing Man; G Toffanin, History of Humanism; C Trinkaus, In Our Image and Likeness; W Bouwsma, The Interpretation of Renaissance Humanism.

L Bouyer ，基督教人文精神； Q布林，约翰卡尔文：一个研究法国人； H西贡，被一个基督教； J旦，真正的人文精神；季封，知民； G Toffanin ，历史人文；特林，在我们的图像和肖像； W Bouwsma ，解读文艺复兴时期的人文精神。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新