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Cells were immediately fixed and processed for immuno?uorescence and tested for translocation ofβ-catenin.β-catenin and target genes of Wnt/β-catenin signaling COX-2, cyclinD1, c-fos, c-Jun mRNA expression were examined by RT-PCR. Saos-2 osteoblastic cells were co-transfected with a wild type or mutant Tcf luciferase reporter gene and Renilla luciferase plasmid. Posttransfection cells were subjected to 1 hour of 12% elongation.

作用1h、2h和4h后，用免疫荧光的方法检测β-catenin的表达和在细胞上定位的变化；用半定量RT-PCR检测β-catenin mRNA表达的变化；用质粒转染的方法检测荧光素酶报告基因Tcf转录的情况；用半定量RT-PCR检测Wnt/β-catenin通路下游基因COX-2，cyclinD1， c-fos， c-Jun mRNA表达的情况；用免疫共沉淀的方法检测β-catenin和E-Cadherin的结合情况。

Results the serum level of mmp-2 in study group was significantly higher than that of controls (p＜0.01). the more critical by classification of child-pugh, the lower serumal mmp-2 level (a grade vs b grade, b grade vs c grade,c grade vs a grade;p＜0.01, p＜0.01, and p＜0.01, respectively).

结果 肝硬化组mmp-2显著高于对照组(p＜0.01、)；但是肝硬化患者血清中mmp-2浓度随child-pugh分级增高显著下降，a、b、c级之间差异均有统计学意义(p均＜0.01)，mmp-2水平a＞b＞c。

The expression of C/EBPαand C/EBPβwere found in the basal cell layer of outer root sheath and sebaceous gland in the new born and maturation phase,The expression in nucleuses of outer root sheat cells decreased with the differentiation but enhanced oppositely in the cytoplasm.

C/EBPα和C/EBPβ在不同发育阶段的毛囊皮脂腺单位中均有表达，定位及表达水平与分化程度密切相关。

Results Interstitial cells of Cajal distributed mainly between the circular and longitudinal muscle layers and in the subserosa in the small intestine. The expressions of c-kit mRNA could b...

明确了Cajal间质细胞在BALB c小鼠小肠中的分布，探讨了c kitmRNA表达情况并进一步提出了研究c kitmRNA表达的方法，为更深入研究Cajal间质细胞奠定了基础。

ObjectiveTo gain insight into the mechanism of iodine and thyroid hormone action in brain development. We have observed the difference of cerebellar morphology between iodine deficient dam rats and the normal ones and have tested whether congenital iodine deficiency could regulate immediate early gene proteins c -fos, c - jun and growth - associated protein - 43 ( GAP - 43) in pup cerebellums.

目的 本研究通过低碘饲料建立碘缺乏大鼠模型，观察子代大鼠在小脑发育期由于缺碘导致的小脑组织形态学改变及c-fos，c-jun蛋白和生长相关蛋白-43(GAP-43)表达变化，为探明碘缺乏影响小脑发育的致病机理提供实验依据。

Through the heat and chemical treatment, the content of C-O and C=O functional groups on the graphite felts surface was increased, resulting the increase of the reaction activity of vanadium redox.

通过化学处理和热处理工艺，改善了表面特性，提高了C—O和C=O基团的含量，增加了电极反应的活性区，降低电极反应的活化过电位。

One valley lied between two peaks(flower beginning period and fruit maturating period).When meeting blood serum,the electron on the ring B-C and A-C of quercetin molecule would move and make -OHs of Ring B and A ionizated and one anion with double negative charge would present.

遇到血清时，槲皮素分子结构中的B环和C环、A环和C环形成的两套共轭体系中的电子，发生转移并使酚羟基解离，形成带双负电荷的阴离子，此阴离子将与人血白蛋白结构中结合位点上的氨基酸残基以离子键的形式结合。

It is suggested that the scission is preceded by an aldol condensation, followed by a phenolic hydroxyl participation with scission of the C---C in the β-hydroxy-ketone formed.

经推论这一断键反应首先是发生羟醛缩合，继以在酚羟基参与下引发烷羰C－C键断裂的；还应指出，也是由于对位羟基的参与，这些烷基对羟苯基酮不形成它们的乙醇缩酮。

He is the author of a number of tutorial books on programming in C, C++, and Java.

他是诸如 c ， c++和 Java 等编程书籍的作者。

Which are rich in vitamin c, vitamin c and is a natural antihistamine agent, if the daily dietary intake of 1000 mg, is enough to prevent the emergence of allergies.

因为其中含有丰富的维生素c，而维生素c正是天然抗组织胺剂，若每天从饮食中摄取1000毫克，就足以防止过敏症的出现。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新