查询词典 Brucella

Objective To establish a new rapid method for the diagnosis of Brucella abortus.

目的建立一种快速诊断牛布鲁氏杆菌病抗原的新方法。

Key words: Brucella abortus;omp25 gene;cloning;prokaryotic expression

流产布鲁氏菌；omp25基因；克隆；原核表达

In order to cluone, seauence and express the outer membrane protein 25 (Omp25) gene sequence of wapiti's Brucella abortus strain ML.

目的 克隆马鹿布氏杆菌ML分离株25kDa外膜蛋白(Omp25)基因，并在大肠杆菌中表达。

There are several distinct pathogens: Brucella abortus, B. canis, B. melitensis, B. suis, whose identifications correlate with their reservoir s, respectively, cattle, dogs, goats and sheep, and pigs.

有若干不同的病原体：流产布鲁氏杆菌、犬布鲁氏杆菌、山羊布鲁氏杆菌、猪布鲁氏杆菌等，其识别与对应的贮藏宿主有关，即分别为牛、犬、山羊和绵羊、猪。

The Omp25 gene of wapiti's Brucella abortus was amplified by RT-PCR and sequenced, Then the interesting gene was subcloned into pET28a for expression. The results showed that the full length of Omp25 gene was 642bp, which encoded 213 amino acids.

运用RT-PCR技术从马鹿布氏杆菌分离株中扩增出Omp25基因，将其克隆入pMD18-T中进行核苷酸序列测定和遗传变异分析，并将目的基因亚克隆到大肠杆菌表达载体pET28a中诱导表达。

There are several distinct pathogens: Brucella abortus, B. canis, B. melitensis, B. suis, whose identifications correlate with their reservoirs, respectively, cattle , dogs, goats and sheep, and pigs.

有若干不同的病原体：流产布鲁氏杆菌、犬布鲁氏杆菌、山羊布鲁氏杆菌、猪布鲁氏杆菌等，其识别与对应的贮藏宿主有关，即分别为牛、犬、山羊和绵羊、猪。

The omp17.3 gene of Brucella abortus was amplified by PCR and then the amplicon was cloned into the eukaryotic expression plasmid pcDNA3.1 to construct a recombinant plasmid pcDNA3.1-omp17.3. Then the recombinant plasmid pcDNA3.1-omp17.3 was transfected into COS7 cells, and the expressed OMP 17.3 was detected by Western-blotting.

采用PCR方法扩增了布鲁氏菌17.3ku外膜蛋白编码基因，并将该基因克隆至真核表达载体pcDNA3.1中，成功构建了真核表达质粒pcDNA3.1-omp17.3.pcDNA3.1-omp17.3转染COS-7细胞后，通过Western-blotting检测到了17.3ku蛋白的瞬时表达。

There are several distinct pathogen s: Brucella abortus, B. canis, B. melitensis, B. suis, whose identifications correlate with their reservoirs, respectively, cattle, dogs, goats and sheep, and pigs.

有若干不同的病原体：流产布鲁氏杆菌、犬布鲁氏杆菌、山羊布鲁氏杆菌、猪布鲁氏杆菌等，其识别与对应的贮藏宿主有关，即分别为牛、犬、山羊和绵羊、猪。

Additionally,this study developed three species-spcific real-time fluorescence quantitative PCR-based assays for rapidly, specificity, sensitivity,detecting Brucella abortus, Brucella melitensis, Brucella suis.

在建立布鲁氏菌属荧光定量PCR检测方法的基础上，根据布鲁氏菌基因组多拷贝插入元件IS711及其下游多态位点，设计了检测牛、羊、猪种布鲁氏菌的引物和牛、羊、猪种特异性荧光探针，优化了牛、羊、猪种布鲁氏菌荧光定量PCR反应体系和条件，建立了牛、羊、猪种布鲁氏菌荧光定量PCR检测方法，组装了牛、羊、猪种布鲁氏菌检测试剂盒，该方法能特异检测上述3个种的布鲁氏菌。

PFGE patterns,which can be used to classify and identify Brucella,can be used in molecular epidemiological research of Brucella.

脉冲场凝胶电泳图谱可对布鲁菌进行遗传学分类鉴定，在布鲁菌的分子流行病学研究中具有重要意义。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。