查询词典 Ba

Study on the Crystallization Process of Labeled GL-7-ACA Acylase CA130 Complex 7β-bromoacetyl amino cephalosporanic acid (BA-7-ACA), an analog of glutaryl-7-amino cephalosporanic acid (GL-7-ACA), can inhibit and specifically alkylate GL-7-ACA acylase (CA130) from Pseudomonas sp. 130, forming a carbon-carbon bond between BA-7-ACA and the C-2 on indole ring of Trp-β4 residue of CA130.Here we reported that BA-7-ACA labeled CA130 (BA-C130) could self-catalyze the hydrolysis of BA-7-ACA during crystallization process. The hydrolysis was confirmed to be a reaction analogous to the one of GL-7-ACA by comparative MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) spectrometry analysis.

二、GL-7-AcA酰化酶CA130标记复合物的结晶过程研究溴乙酰氨基头孢烷酸（7β-bromoacetyl amino cephalosporanic acid，BA-7-ACA）作为戊二酰-7-氨基头孢烷酸（GL-7-ACA）的类似物，不仅能够抑制GL-7-ACA酰化酶CA130的活力，而且能通过在BA-7-ACA和CA130的β亚基第四位色氨酸吲哚环二位碳原子之间形成碳-碳共价键而将CA130特异的烷基化。

B Of the six basic media MS, MS1/2 (half-strength of MS salts and vitamins), WPM, DKW, B5 and SH, MS1/2 was the most proper one to induce somatic embryos. Somatic embryos generally regenerated directly from excised zygotic cotyledons. PGRs combination affected somatic embryogenesis significantly. Medium with NAA 1mg/L, TDZ 0.05mg/L, IBA 2—10mg/L combined with BA 10mg/L, or IBA 10mg/L integrated with BA 0-2mg/L gave the highest induction rate. Excised zygotic hypocotyls had the strongest potential to produce callus. Callus induction was also affected significantly by media and PGRs. The proper callus induction condition was MS1/2 medium containing NAA 1mg/L, IBA 10mg/L, BA 2-5mg/L and TDZ 0.05mg/L. Harvest period affect somatic embryogenesis significantly. Zygotic embryo explants collected from the end of July to the middle of August had strong potential to generate somatic embryos, when endosperm finished solidification, different parts of the embryos were completely formed, the size of embryos occupied about 2/3 of the embryo sac. Provided with optimized conditions, direct somatic embryogenesis rate can attain to 33. 68%, and callus induction rate of hypocotyls was up to 90.7%. Cytological observation on megasporogenesis and zygotic embryogenesis of Manchurian ash showed that the ovary was twicarpellum, twilocular with two ovules each loculus. The ovule was tenuinucellar and anatropous, with one megasporcocyte. The development of embryo sac is of the Polygoum type.

体细胞胚胎发生研究的结果表明：(1)成熟过程中的合子胚是诱导水曲柳体细胞胚胎发生的最佳外植体材料；(2)在所试验到的MS、MS1/2（将MS的所有成分均减半）、WPM、DKW、B〓、SH等六种基本培养基中，MS1/2是最适合诱导水曲柳体细胞胚胎发生的基本培养基；(3)水曲柳的体细胞胚胎发生以直接发生为主，体细胞胚主要来自于从合子胚分离的完整子叶；(4)培养基中的激素组合对水曲柳的体细胞胚胎发生有显著影响，诱导直接体细胞胚发生较好的激素组合有NAA 1mg/L+IBA 2，5，10mg/L+BA 10mg/L+TDZ 0.05mg/L和NAA 1mg/L+IBA 10mg/L+BA 0，2mg/L+TDZ 0.05mg/L；(5)合子胚分离的下胚轴具有最强的愈伤组织诱导潜力，少数愈伤组织可以分化出体细胞胚；(6)愈伤组织的诱导也受培养基和激素配比的显著影响，最适宜诱导的培养条件为MS1/2+NAA 1mg/L+IBA 10mg/L+BA 2，5mg/L+TDZ0.05mg/L；(7)采种时间对体细胞胚胎发生有显著影响。7月末到8月中旬的合子胚具有较强的体细胞胚发生潜力，此时种子尚未成熟，胚乳已呈固态，种胚的各个部分已分化完全，种胚体积占胚腔的大约2/3；(8)在各自综合的最适条件下，完整子叶的体细胞胚诱导率可达33.68%，下胚轴的愈伤组织诱导率可达90.7%。

It turned out reproduction rete of chrysanthemum was raised,the regular,non-plant diseases and insect pests could be offered to the market. Shoot tip were used as explants to study the effects of different sterilization methods, illumination intensity and concentrations of 6-BA and NAA on shoot propagation. Rooting with perlite, vermiculite and river sand instead of agar was studied. In the process of preliminary multiplication, the effect of using 0.1% HgCl_2 in sterilization of explants was better than using saturated bleaching solution. The highest multiplication rate was found under whole illumination. MS media with 6-BA from 0.2 mg·L~(-1) to 5 mg·L~(-1) and NAA from 0.05 mg·L~(-1) to 2 mg·L~(-1) were tested and as a result, the highest differentiation rate and the multiplication rate was reached with 0.2 mg·L~(-1) 6-BA, 0.05 mg·L~(-1) NAA.

本实验以菊花的茎尖为外植体，研究了不同灭菌方法，光照强度，全光照、半光照和弱光处理以及不同6-BA和NAA浓度及配比对于试管苗增殖的影响，并研究了珍珠岩、蛭石和河砂等琼脂替代物对于菊花生根的影响，结果表明：在初代培养物建立的过程中采用0.1％升汞进行表面灭菌的效果好于饱和漂白粉的灭菌效果；在全光照条件下外植体的增殖倍数最高；在试管苗增殖培养的过程中，以MS为基本培养基，并在培养基中添加0.2～5mg·L~(-1)6-BA和0.05～2mg·L~(-1)NAA，其中0.2mg·L~(-1)6-BA和0.05mg·L~(-1)NAA最适于外植体的分化和增殖，其分化率为100％，增殖倍数为12。

However,the combination of BA with IAA promoted thededifferentiation and redifferentiation,though the regeneration frequency differed in kindsof combinations,among which the combinations of 2.0-3.0 mg/l BA + 0.5 mg/l IAAproduced a highest differentiation frequency of 70％or so.

在仅添加了BA的分化培养基上，下胚轴脱分化和再分化都比较困难，而BA和IAA配合使用时却能很好地促进下胚轴的脱分化和再分化。不同BA和IAA配比组合中下胚轴的不定芽分化率存在差异，其中以BA2.0-3.0mg/l+IAA 0.5mg/l组合的不定芽分化率最高，均达到了70％左右。

For the bud directly differentiation , the suitable culture medium was MS+BA 2mg/L +NAA 0.2mg/L or MS+TDZ0.01mg/L +NAA0.2 mg/L, of which the induction rate was 88% and 100% respectively. An efficient tissue culture system has been developed with the bud of mature seed of Aesculus hippocastanum L. as explants . Buds were induced from 2cm high young plantlet cultured on MS medium supplemented with 0.6 mg/L 6-BA plus 0.1mg/L or 0.4~0.6mg/L ZT plus 0.1 mg/L NAA for 15 d, and the induction rate was 100%, the mean No. of buds was 35.7; The combination of MS+0.2mg/L 6-BA +0.1mg/L NAA + 10mg/L AD was the suitable culture medium for elongation of the buds.

以欧洲七叶树成熟种子的胚芽进行离体培养和快速繁殖，结果表明：高约2cm的无菌苗在MS+0.6mg／L 6-BA+0.1 mg／L NAA或MS+0.4～0.6mg／L ZT+0.1 mg／L NAA培养基上培养15 d左右可诱导出不定芽，分化频率为100％，平均每株产芽35.7个；MS+0.2mg／L 6-BA+0.1mg／L NAA+10mg／L AD培养基有利于芽伸长；生根培养基为1／2MS+0.4 mg／L NAA+0.2mg／L IBA时，生根率可达75％。

Don.methodswith the stem of sterile seedling and fresh plants as exophyte, ms as a basic medium, 6-ba and naa hormone were adopted as the experimental variables.resultsms +1.0 mg / l 6-ba +1.0 mg / l naa induced the best effect of callus; higher concentrations of 6-ba with high concentrations of naa made callus densification and higher rate.conclusion different plant hormones and their different concentration ratios have different effects on the callus of halenia elliptica d.

方法以椭圆叶花锚种子培养的无菌苗的茎和新鲜植株的茎段为外殖体，ms为基本培养基，分别以6-ba和naa两种植物激素为变量进行实验研究。结果ms培养基+1.0 mg/l 6-ba+1.0 mg/l naa对诱导椭圆叶花锚愈伤组织的形成效果最佳；高浓度的6-ba配合较高浓度的naa诱导的愈伤组织致密，出愈率高。

The increment of 6-BA concentration can increase the protocorm propagation obviously. And the matching of high concentration of 6-BA and low concentration of NAA is more suitable for the protocorm propagation of C. grandiflorium. 6-BA at the concentration of 1.5 mg/L was most favorable for protocorm induction and differentiation.

结论］6-BA浓度的增加，可明显提高原球茎的增殖，高浓度6-BA与低浓度NAA的配比较适合大花蕙兰的原球茎增增殖；6-BA在1.5 mg/L时最有利于原球茎的诱导分化。

Result The optimum medium formula for protocorm propagation in tissue culture of C. grandiflorium was MS+0.1 mg/L 6-BA. The suitable medium for protocorm induction and differentiation in tissue culture of C. grandiflorum was WS+1.5 mg/L 6-BA+0.3 mg/L NAA with the bud-inducing rate up to 130%. The suitable medium for root inducement was 1/2MS+0.2 mg/L NAA+1.0 mg/6-BA and the survival rate of in vitro plantlets after transplanting was 78%.

结果］大花蕙兰组织培养原球茎粉殖以MS+0.1 mg/L NAA+2.0 mg/L6-BA培养基配方最好；适合大花蕙兰组织培养原球茎诱导分化的培养基配方为MS+1.5 mg/L6-BA+0.3 mg/L NAA，诱导出芽率可达130%；适合大花蕙兰诱导生根的培养基配方为1/2 MS+0.2 mg/L NAA+1.0 mg/L6-BA，试管苗移栽后成活率为78%。

In order to study the changing process of Barium strontium titanate Ba(subscript xSr(subscript 1-x) TiO3, BST from cubic phase to the tetragonal phase with Ba doping and the ferroelectric characteristic of BST, the total energy of BST with different mole ratio of Ba/Sr when Ba doped was calculated and the fine structure determined, based on general gradient approximation, by means of ultrasoft pseudopotentials plane wave method.

为了研究钛酸锶(SrTiO3， ST)中随Ba的掺入，其晶体从顺电相到铁电相的变化过程及四方相钛酸锶钡Ba(下标 xSr(下标 1-x)TiO3， BST的铁电性。在广义梯度近似下，利用超软赝势平面波方法计算了不同Ba/Sr摩尔比BST的总能量，确定了BST的精细结构。

The majority of BA tested didn′t contract to NE, only a small number of BA contracted to NE but with obvious lower afficacy, lower affinity and tachyphylaxis to NE than RA.

按研究BA和RA平滑肌V〓受体时我们发现，尽管BA和RA在V〓受体的分布上没有差别，但在无钙介质中，AVP引起的收缩在BA则被明显抑制，而在RA则仅被轻度抑制。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失