查询词典 BP

The mRNA of pituitary glands from seven important economic Pleuronectiformes fishes was extracted,the cDNA sequences of GH gene were then isolated from Cleisthenes herzensteini,Limanda yokohamae,Pleuronichthys Cornutus,Cynoglossus robustus,Platichthys bicoloratus, Zebrias zebra and Paralichthys dentatus with RT-PCR method by using designed specific primers based on the reported GH cDNA sequences of Pleuronectiformes. The results of sequencing showed that lengths of the above cDNAs are as follows: 479 bp,564 bp,519 bp,440 bp,564 bp,440 bp and 522 bp.The GH cDNA sequences encode mature polypeptide of about 140 to 170 amino acid.The full-length growth hormone cDNAs of Paralichthys lethostigma and Scophthalmus maximus were cloned by Switching Mechanism At 5\' end of the RNA Transcript RACE technology.

从7种重要鲽形目经济鱼类——高眼鲽、黄盖鲽、木叶鲽、宽体舌鳎、石鲽和条鳎、夏鲆的脑垂体中提取mRNA，并采用RT-PCR方法，根据已克隆得到的鲆鲽鱼类生长激素基因的保守性序列设计特异性引物，克隆了含开放阅读框的生长激素(Growth Hormone，GH)cDNA序列，测序结果显示，高眼鲽、黄盖鲽、木叶鲽、宽体舌鳎、石鲽、条鳎和夏鲆的GH cDNA长度依次为479 bp、564 bp、519 bp、440 bp、564 bp、440 bp和522 bp，编码140-170个氨基酸的成熟GH多肽片段；从漠斑牙鲆和大菱鲆脑垂体中提取mRNA，利用SMART-RACE技术建立脑垂体cDNA文库，并从该文库中克隆出大菱鲆和漠斑牙鲆生长激素基因cDNA全长序列。

The conserved regions of rRNA and tRNA genes exhibited higher sequence similarity among plant species. Five primer pairs were designed from the conserved regions distributing in psbA~trnK, psbB~psbH, rpl23~trnI, trnR-ACG~trnN-GUU and trnY-GUA~trnD-GUC, respectively to amplify sequences about 500~600 bp, 800~900 bp, 650~700 bp (350 bp, not legume species), 700~800 bp (400 bp in dicotyledon), and 500~600 bp.

经由上述比对分析后设计出5引子对，位於叶绿体基因体的位置分别为psbA~trnK、psbB~psbH、rpl23~trnI、trnR-ACG~trnN-GUU及trnY-GUA~ trnD-GUC，预期实际应用在其他物种上所能增幅之变异序列长度分别约为500~600 bp、800~900 bp、650~700 bp (豆科以外物种为350 bp)、700~800 bp (双子叶植物为400 bp)及500~600 bp。

There are four main topics in this paper:(1) improvement of BP networks algorithm;(2) improvement of BP networks structure, and applying the improved BP networks to the prediction of the time series with certain increasing or decreasing tendency;(3) using the BP networks to diagnose the vibration faults of steam turbines;(4) prediction on the cleanliness rate of steam condenser and the fuzzy diagnosis for condenser based on BP networks.

本文主要研究了四个问题：(1)完善和改进了BP网络的训练算法；(2)BP网络结构的改进研究，并将其应用于汽轮机故障这类有确定性递增趋势的时间序列的预测中；(3)BP网络应用于汽轮机的振动故障诊断研究；(4)基于BP网络的凝汽器清洁率的预测方法及模糊诊断研究。

The polymorphism information content were 0.6674, 0.6035, and 0.5615, respectively. The total mean of litter size of genotype LSCV043 107 bp/123 bp was significantly higher than that of genotype 110 bp/123 bp. The liner size of the first lambing for genotypes BMS2508 154 bp/154 bp, 154 bp/170 bp and 154 bp/200 bp were significantly higher than that of genotype 170 bp/170 bp (P.05). Furthermore, total litter size for BMS2508 170 bp/170 bp was the lowest in all the genotypes within this locus (P.05). No significant difference was detected in other genotypes.

对不同基因型群体的产羔率进行统计分析，LSCV043基因型为107 bp/123 bp所对应的总体产羔数明显高于110 bp/123 bp基因型群体所对应的产羔数(P.05)；BMS2508基因型为154 bp/154 bp、154 bp/170 bp和154 bp/200 bp所对应的群体第一胎产羔数明显高于170 bp/170 bp所对应的产羔数(P.05)，基因型170 bp/170 bp的群体总体产羔数均低于该位点的其他基因型(P.05)；其他位点各基因型之间产羔数均无显著差异。

The result of sequence analysis indicated that the length of cDNA and DNA fragments of Lectin gene and the length of cDNA and DNA fragments of Pectate Lyase gene were 508 bp,575 bp,1,316 bp and 1,858 bp respectively.The DNA fragment of Lectin gene had a intron,whose length was 67 bp .The DNA fragment of Pectate Lyase gene had 3 introns,whose lengths were 90 bp,83 bp,and 370 bp respectively. All these four introns sequences followed the rule of GT-AG.

通过序列分析得知，香蕉凝集素基因cDNA片段长508 bp；香蕉凝集素基因DNA片段长575bp，有一个长为67bp的内含子；香蕉果胶裂解酶基因cDNA片段长1，316bp；香蕉果胶裂解酶基因DNA片段长1，858bp，有三个内含子区，分别长90 bp，83 bp和370bp；在总共的四个内含子序列中，分别都符合GT-AG规则。

At last, by the simulation results and root-mean-square value, the traffic FIRTF neural networks learning and prediction owned on a better advantages, because that the traffic flow FIRTF and the adaptive algorithm combination integrated gda-BP networks, gdm-BP networks, gda-feedback-BP networks and gdm-feedback-BP networks of the four characteristics.

最后，通过交通流量时间序列数据进行了试验分析，说明交通流量FIRTF神经网络以及其于混沌机理的交通流量FIRTF自适应算法综合了gda-BP网络、gdm-BP网络、gda-feedback-BP网络和gdm-feedback-BP网络的特点，使得交通流量FIRTF神经网络在训练和预测的速度上占有很大的优势。

Results are as followed:1 Exposure of HELF cells to BP caused c-Jun activation,and increased the activity of MAPK,PI-3K,p53 and cyclin D1 pathway.2 BP-induced c-Jun activation was inhibited by dominant negative mutants of extracellular signal-regulated protein kinase or c-Jun NH_2-terminal kinase,but not by p38,impling that JNK and ERK pathways medicate c-Jun activation induced by BP.3 Overexpression of dominant-negative mutants PI-3K and Akt potently blocked phosphorylations of c-Jun and ERK,but not JNK in response to BP,suggesting that PI-3K/Akt pathway positively regulates BP-induced c-Jun activation through ERK.4 Inhibition of p53 by its chemical or molecular inhibitor markedly increased the phosphorylation levels of c-Jun,Akt and ERK upon BP stimulation,indicating that p53 negatively medicates BP-induced c-Jun activation through PI-3K/Akt/ERK pathway.5 The cell lines expressed TAM67 exhibits no significant affecting normal cell growth properties.6 TAM67 was able to significantly block G_1-S transition and subsequent cell proliferation,suggesting that c-Jun is essential for cell cycle alternations elicited by BP.7 Overexpression of TAM67 impaired BP-induced cyclin D1 activation,decreasing expression of E2F1 and pRb,indicating that c-Jun participates in the modulation of BP-induced activation of cyclin D1/pRb/E2F1 pathway.8 Stably expression of TAM67 led to the increases in the expression levels of p53 and p21,elevating phosphorylation level of p53,clearly indicating that c-Jun regulates p53/p21 pathway activation induced by BRCollectively,PI3K/Akt/ERK pathway mediated BP-induced c-Jun activation through p53-dependent mechanism.

结果显示：1BP刺激细胞可促进c-Jun活化，并伴随着MAPK、PI-3K、p53和cyclinD1通路各组成成分的活性增强。2利用MAPK通路的显性失活突变体分别阻断细胞外信号调节激酶和c-Jun氨基末端激酶活性，均可明显抑制BP诱导的c-Jun活化，但阻断p38活性对BP引起的c-Jun活化无明显影响，提示JNK和ERK通路参与调控BP诱导的c-Jun活化。3过表达PI-3K和Akt的显性失活突变体也可显著抑制BP诱导的c-Jun活化，并降低磷酸化ERK的表达水平，但对磷酸化JNK的表达水平无明显影响，说明PI-3K/Akt通路通过ERK正性调控了BP诱导的c-Jun活化。4p53的化学/分子抑制剂能使BP作用的细胞内c-Jun活性明显增加，并同时诱导Akt和ERK的磷酸化水平的升高，表明p53可通过PI-3K/Akt/ERK通路对BP诱导的c-Jun活化进行负性调控。5随后观察转染细胞的生长情况，发现TAM67对细胞正常生长和形态无明显影响。6稳定表达TAM67可有效抑制BP诱导的S期细胞数的增加，提示c-Jun在BP致细胞周期改变的过程中发挥了重要作用。7TAM67过表达能够抑制BP诱导的cyclin D1活化，降低磷酸化Rb以及E2F1蛋白表达水平，表明c-Jun参与调控BP诱导的cyclin D1/Rb/E2F1通路的活化。8过表达TAM67可使BP刺激的细胞中p53、p21总蛋白以及p53磷酸化的表达水平明显升高，可见c-Jun也参与调控BP诱导的p53/p21通路活化。

Four rapid depositional periods such as 12.3~12.1 ka BP, 7.5~7.3 ka BP, 5.9~5.2 ka BP, and 1.5~1.3 ka BP disclosed by Core EC2005 well correspond to the worldwide Younger Dryas, 7.3 ka cold event, Neoglaciation or 5.5 ka cold event, and 1.4 ka ice rafted debris event, respectively. The worldwide 8.2 ka event is also well recorded in the core.

东海内陆架浙-闽沿岸泥质带EC2005孔存在的4个百年时间尺度上快速沉积事件：12.3~12.1 ka BP、7.5~7.3 ka BP、5.9~5.2 ka BP和1.5~1.3 ka BP，与新仙女木事件、7.3 ka BP冷事件、第二新冰期(5.5 ka BP强降温事件)以及北大西洋1.4 ka BP浮冰事件均有良好的对应，世界范围内广泛存在的8.2 ka冷事件对本钻孔沉积也形成了一定影响。

From 6860 to 3170 cal.yr BP,semi-desert steppeexpanded,regional montane forest steppe retreated,suggesting a prolonged warm and dryclimate.Between 3170 and 2340 cal.yr BP,regional forest steppe expanded whereassemi-desert steppe retreated,indicating the climate became cool and wet gradually and thehumidity reached the maximum at the end of this stage.During 2340～1600 cal.yr BP,Poaceae steppe dominated whereas wetland meadow expanded and the montane forest stepperetreated,suggesting a cool and wet climate prevailed,wormwood grass steppe prevailed andthe climatic instability increased after 1600 cal.a BEThe climate and environmental reconstructions by pollen were supported by the diatomrecords.A relative high lake level and moister climate prevailed between 8660 and 7230 cal.yr BP as inferred by the dominance of planktonic diatom genera and high planktonic/benthicratio (〉40).low lake level and dry climate sustained during 7230～2330 cal.yr BP assuggested by the low abundance of planktonic diatom and low planktonic/benthic ratio ～20and the lake returned to high level during 2330～930 cal.yr BP as inferred by there-dominance of the planktonic diatom and the high value of palnktonic/benthic ratio.The pollen-based climate reconstruction was also consolidated by the grain size andPediastrum concentration records.

Ugii Nuur湖周围现代孢粉研究表明：松属为空气传播的外来花粉，其含量变化可能指示了远山森林草原发育状况；禾本科具显著低代表性，母体植物建群时含量通常在10％以下：藜科花粉含量可以指示区域气候干旱程度。2、以现代孢粉研究为基础、可靠AMS~(14)C年代为框架，Ugii Nuur孢粉记录高分辨率地重建了蒙古国中部地区过去8660 cal.yr BP以来的植被和气候变化。8660～7800 cal.yr BP研究区植被为禾草草原，湖畔及河谷低地发育苔草草甸，远山发育松属针叶林和森林草原，气候温和略湿润；其中8350～8250 cal.yr BP湿生草甸扩张，旱生植物成分减少，气候凉湿。7800～6860 cal.yr BP旱生成分增加，气候转向暖干。6860～3170 cal.yrBP半荒漠草原扩张，远山森林草原退缩，山地草原和河谷及湖畔湿生草甸退缩，气候持续暖干。3170～2340 cal.yr BP半荒漠草原退缩，远山森林草原扩张，气候转凉变湿，末期湿度条件达最佳。2340～1600 cal.yr BP禾草草原发育，湖畔及河谷低地湿生草甸扩张，远山森林草原略有退缩，气候总体较凉湿。1600 cal.yr BP以来研究区发育禾草-冷蒿草原，气候波动较大。

There is an increasing need to better understand the fate of domoic acid, in aquatic ecosystem. In this report, partial cDNA of hepatic cytochrome P-450 1A (CYP1A), giutathione S-transferase alpha, glutathione S-transferase rho, heat shock protein 70 (HSP70), Na~+/K~+-ATPase alpha 1(ATP 1A1) and beta-actin gene from rabbitfish were obtained by PCR using degenerate primers, which respectively were 900 bp, 582 bp, 615 bp, 660 bp, 749 bp and 554 bp in length.

本研究成功克隆了黄斑篮子鱼肝脏第Ⅰ时相去毒酶细胞色素P450 1A，第Ⅱ时相去毒酶谷胱甘肽S-转移酶alpha和谷胱甘肽S-转移酶rho(GS7R)，热休克蛋白70(HSP70)，钠钾ATP酶alpha 1(ATP1A1)和β-肌动蛋白基因的核心序列，它们分别长900 bp，582 bp，615 bp，660 bp，749 bp和554 bp。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。