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Methods:All of 40 male Sprague-Dawley rats were divided into 3 groups:control group(group A, n = 10), diabetic group for 4 weeks(group B, n = 14), and diabetic group for 12 weeks(group C, n = 16). The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NF-κB was detected by electrophoretic mobility shift assay.

将成年雄性SD大鼠40只分为3组:A组为正常对照组(10只),B组为糖尿病4周组(14只),C组为糖尿病12周组(16只),采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4周后,A组、C组大鼠于12周后通过注射阿朴吗啡后评价勃起功能,并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达,同时采用电泳迁移率变动分析法进行NF-κB 活性检测。

Methods All of 40 male Sprague-Dawley rats were divided into 3 groups: control group (group A, n=10), diabetic group for 4 weeks (group B, n=14), and diabetic group for 12 weeks (group C, n=16). The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NE-κB was detected by electrophoretic mobility shift assay.

将成年雄性SD大鼠40只分为3组:A组为正常对照组(10只),B组为糖尿病4周组(14只),C组为糖尿病12周组(16只),采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4周后,A组、C组大鼠于12周后通过注射阿朴吗啡后评价勃起功能,并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达,同时采用电泳迁移率变动分析法进行NF-κB活性检测。

Even when the competition started with 87% of non-B biotype and 13% of B biotype in numbers, the non-B biotype was completely displaced in 225 days.

在棉花上,即使以非B型占87%、B型占13%开始共存竞争,经过225d后,非B型也完全被B型替代。

Results During the operation, the breath and circulation in group A is more steadily than that in group B (P.05); the incidence of bodymovement and larynx spasm and the dosage of ketamine are lesser than that in group A. Postoperative children recovery is faster and more steadily in group A than those in group B.

结果 A组术中血压、心率和呼吸较接近麻醉前基础值,且术中波动较小,生命体征平稳。B组血压、心率及呼吸率均较术前明显增加,且始终保持在较高水平,随麻醉深浅而有显著波动。A组循环、呼吸功能较B组稳定(P.05);肢体不良自主运动,喉痉挛等不良反应较B组少(P.05);A组氯胺酮用量明显少于B组(P.01);术后A组苏醒较快且平稳,恢复期精神症状少,与B组比较差异有统计学意义(P.05)。

According to the number and behavior of B genomechromosomes, all hybrid plants were classified into three types. All somatic cells of TypesⅠplants contained 16 B genome chromosomes which formed 8Ⅱat diakinesis and shown8:8 segregation at anaphase; TypesⅡcontained 14 B genome chromosomes whichformed 6Ⅱ+2Ⅰat diakinesis and showed 6:8 or 7:7 segregation at anaphase. TypesⅢcontained above two chromosomes number and behavior.

同时根据B基因组DNA为探针的GISH结果,以细胞中B基因组染色体的数目及减数分裂时的配对及分离的方式,将所有杂种植株分为三大类:Ⅰ类具有16条B基因组染色体,以8Ⅱ的方式配对,以8:8分离,共32株;Ⅱ类具有14条B基因组染色体,以6Ⅱ+2Ⅰ方式配对,以7:7或者6:8的方式分离,共4株:Ⅲ类植株包含有上述两种细胞类型类型,共1株。

Itis a great deal of assistment for treatment and prognosis of patients, if we candiagnose hepatic fibrosis in forepart. The aim of this experiment evaluate the dignosisvalue of ultrasound elastic imaging on early hepatic fibrosis.we will supply thefundermental data for reseach henceforth. 2、Materials and Method The number of Wister rats is 30 and weight is 180-220g. 30 Wister rats had beenligated on the right hepatic ducts (include middle lobe、right lobe and caudate lobe) tocause partial cholestatic hepafibrosis factitiously and divided in A, B and C groups. Aand B groups had been ligated on the right hepatic ducts both. The different with Aand B is that the time of A group after ligation on the hepatic ducts is 2 weeks and thetime of B group is 1 week.

将实验动物分为A、B、C三组,A组为结扎大鼠右侧胆管(包括中叶、右叶、尾状叶)2周后进行检查。B组为结扎大鼠右侧胆管1周后进行检查,C组行假手术为对照组。A组大鼠麻醉后,腹部备皮,皮肤常规消毒,腹部横行切开,暴露肝脏、胆总管,游离右侧胆管,结扎,然后关腹;C组,仅开腹然后关腹,即假手术组。A、C两组同批实施手术,A、C两组术后1周,B组实施手术,步骤、方法、部位同A组。

Itis a great deal of assistment for treatment and prognosis of patients, if we candiagnose hepatic fibrosis in forepart. The aim of this experiment evaluate the dignosisvalue of ultrasound elastic imaging on early hepatic fibrosis.we will supply thefundermental data for reseach henceforth. 2、Materials and MethodThe number of Wister rats is 30 and weight is 180-220g. 30 Wister rats had beenligated on the right hepatic ducts (include middle lobe、right lobe and caudate lobe) tocause partial cholestatic hepafibrosis factitiously and divided in A, B and C groups. Aand B groups had been ligated on the right hepatic ducts both. The different with Aand B is that the time of A group after ligation on the hepatic ducts is 2 weeks and thetime of B group is 1 week.

将实验动物分为A、B、C三组,A组为结扎大鼠右侧胆管(包括中叶、右叶、尾状叶)2周后进行检查。B组为结扎大鼠右侧胆管1周后进行检查,C组行假手术为对照组。A组大鼠麻醉后,腹部备皮,皮肤常规消毒,腹部横行切开,暴露肝脏、胆总管,游离右侧胆管,结扎,然后关腹;C组,仅开腹然后关腹,即假手术组。A、C两组同批实施手术,A、C两组术后1周,B组实施手术,步骤、方法、部位同A组。

The notable proliferation was not observed by eyes in the local of injection. The infiltration of inflammation cells and mild proliferation of fibrocyte around dura mater was observed by HE stained in 4 and 8 weeks after injection. Infiltration and exudation of inflammation cells was observed by HE stained in epidural nerve root. Compared with group A, no changes of group B, C and D were observed under specific stained. Proliferation of type Ⅱ collagen fibers around dura mater was seen under immunohistochemical stained in 4 and 8 weeks after injection. There is no significant demyelination changes under LFB stained. The thickness and shape of the myelin sheath in epidural nerve root was not regular under transmission electronic microscopy in 4 and 8 weeks after injection. Fibroblast was also seen there. In nerve endometrium, macrophage could be seen under TEM, myelinated nerve fiber changed significantly, but nonmyelinated nerve fiber changed mildly. When 8 weeks, the changes of group D is smaller than the group B and C.

给药局部肉眼观察未见明显的纤维组织增生;HE染色可见B、C、D三组给药后四周及八周时硬膜内外均有炎细胞浸润,纤维细胞轻度增生,硬膜外神经根内有炎细胞浸润及炎性渗出;特殊染色B、C、D三组同A组相比未见有脊髓及神经根的改变;免疫组化染色,给药后四周及八周时,硬膜内外均有Ⅱ型胶原纤维增生;固兰染色B、C、D三组未见有明显脱髓鞘改变,与A组相比无明显异常改变;电镜观察B、C、D三组在给药后的四周及八周时,表现为硬膜外神经根内髓鞘厚薄不一,形状不规则,可见成纤维细胞,神经内膜中可见有巨噬细胞;粗大的有髓神经纤维变化明显,无髓神经纤维受累较轻;八周时电镜下D组改变较B、C两组为轻。

The French and Italian poets gave preference to the Italian form of sonnet—two groups of four lines, or quatrains (always rhymed a-b-b-a a-b-b-a) followed by two groups of three lines, or tercets (variously rhymed c-c-d e-e-d or c-c-d e-d-e)—which created a sonorous music in the vowel rich Romance languages, but in Shakespeare it is artificial and monotonous for the English language.

法国和意在利诗人优先使用意大利格式的商籁诗----两组四行诗(总是按a-b-b-a a-b-b-a的格式押韵)加上两组三行诗(按c-c-d e-e-d或 c-c-d e-d-e格式押韵),这就在元音丰富的罗马语言中产生了一种感人的音乐节奏,然而放在莎士比亚的英语作品里,就变得矫揉造作和枯燥单调。

Results Group C had the symptoms such as reduced activity, acceded, indulge in lying and weight loss after 3 weeks of immune injection, 14 out of 16 SD rats in Group B had the same symptoms as Group C after 4~5 weeks of immune injection, the serum enzymes in model groups increased significantly compared with those of the control group, model group C was much higher than model group B; the duration shorted, amplitude decreased, multiphase wave increased in electromyogram of model groups; MRI examination revealed samples from model group B and C had one positive case each, which presented T1MI isodensity or hypodensity signal, T2MI and STIR serial hyperdensity signal, revealing muscle inflammation; all rats'skeletal muscle from model group C and 11 out of model group B had pathological changes, which exhibited striated muscle focal fiber degeneration, necrotized and inflammatory cell infiltration, interstitial vessel wall thickening, random cardiac muscle samples had 3 positive changes, which had similar changes to skeletal muscle, there was 1 positive change from lung sample.

结果:模型C组于免疫注射第3周左右开始出现活动减少,倦怠嗜卧,食欲体重下降等表现,模型B组有14只SD大鼠于免疫注射第4~5周出现上述症状,较C组为轻;模型组肌酶谱中,肌酸激酶、乳酸脱氢酶、谷草转氨酶与对照组比较明显升高,模型C组较模型B组升高更显著;模型组肌电图时限缩短,波幅降低,多相波增多;磁共振检查模型B组和C组选送标本中各有一例有阳性改变,表现为T1MI等或稍低信号,T2MI及STIR序列为高信号,提示肌肉炎症水肿改变;模型B组有11只,模型C组全部大鼠骨骼肌出现病理改变,表现为横纹肌局灶性分布的肌纤维变性炎细胞浸润,间质小血管壁增厚、扩张,随机选送的心肌标本中有3例有阳性改变,表现与骨骼肌相仿,选送的肺标本中有1例有阳性改变,表现为蛋白渗出,炎症细胞浸润和小血管改变。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

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