查询词典 B-battery

One of the suites, for example, exhibits the musical refrain B-flat, A, C, B, which in German usage spells Bach.

比如组曲中其中一个曲子有一个&降B，A，C，B&的反复，而这四个字母排列恰巧是&巴赫&的德语拼法。

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At present the most successful operation of the food B to B-type e-commerce model.

目前运作最成功的粮食B to B型电子商务模式。

Without waiting for Bs ball to come to rest, A knocks Bs ball back to B.

没有等到B的球停下来，A就把B的球击打回到B那儿。

A novel chiral block copolymer PCys-b-PPO-b-PCys bearing Leucine in the side chain was synthesized for the first time.

首次合成了侧链带有手性基团的聚半胱氨酸－b-聚环氧丙烷－b-聚半胱氨酸嵌段共聚物。

Novel chiral block copolymer PCys-b-PPO-b-PCys bearing Leucine in the side chain was synthesized for the first time.

bstract 首次合成了侧链带有手性基团的聚半胱氨酸－b-聚环氧丙烷－b-聚半胱氨酸嵌段共聚物。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

According to the structure characteristics of L-B composite brake beam, the finite element model of L-B brake beam based on contact is established, and the stress distribution of the brake beam in different conditions calculated is using I-DEAS software. Calculated results show that the stress concentration mainly generates in the root of brake header slider, which fit with the faults in actual operation, and provide reliable basis to improve design and optimization.

根据L-B型组合式制动梁的结构特点，运用I-DEAS软件建立基于接触的L-B型制动梁有限元模型，并计算了制动梁在不同工况下的应力分布，计算结果表明应力集中主要发生在闸瓦托滑块根部，这与实际运行中出现的故障较为吻合，为制动梁的改进设计和结构优化提供了依据。

Methods 100 examples,randoms choosing scheduled time line underbelly operation (operation,the womb cut completely or satisfy time eager operation,ovary tumour operation including cutting the palace product open) divide into two set,a forms:give hard film outside rentaini 0.4 mg + 0.75% bupivacaine 20 ml formulating cheng 100 ml analgesias,b group behind the skill:100 ml compounding cheng ease pain,comparatively,the life body collects,optesthesia simulates grade and bad reaction in giving hard film outside rentaini 0.4 mg + interest rate of 0.75% bupivacaine 20 ml + fluorines pai many 2 ml after the skill.results two set of patient skill queens circulation stabilizes equally,skill queen vas difference has no statistics significance,two set of analgesic effects have no notable difference,b many by-reaction of fluorine pai benefit appears on group but.

择期行下腹部手术(包括剖宫产手术、子宫全切或次全切手术、卵巢肿瘤手术)100例，随机分成两组，a组：在术后予硬膜外芬太尼0.4mg+0.75%布比卡因20 ml配制成100 ml镇痛。b组：在术后予硬膜外芬太尼0.4mg+0.75%布比卡因20ml+氟哌利多5mg配制成100ml镇痛，比较生命体征、视觉模拟评分和不良反应。结果两组患者术后循环均稳定，术后vas差异无统计学意义，两组镇痛效果无显著差异，但b组出现氟哌利多不良反应。

The highest content of HCB is 6,254 times higher than the B level standard set by the Ministry of Environmental Protection, carbon trichloride 9,330 times higher, hexachlorobutadiene 290.5 times higher.

六氯苯最高超出环保部B级标准6254倍，六氯乙烷最高超出环保部B级标准9330倍，六氯丁二烯最高超出环保部B级标准290.4倍。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。