查询词典 B-S

Novel chiral block copolymer PCys-b-PPO-b-PCys bearing Leucine in the side chain was synthesized for the first time.

bstract 首次合成了侧链带有手性基团的聚半胱氨酸－b-聚环氧丙烷－b-聚半胱氨酸嵌段共聚物。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

According to the structure characteristics of L-B composite brake beam, the finite element model of L-B brake beam based on contact is established, and the stress distribution of the brake beam in different conditions calculated is using I-DEAS software. Calculated results show that the stress concentration mainly generates in the root of brake header slider, which fit with the faults in actual operation, and provide reliable basis to improve design and optimization.

根据L-B型组合式制动梁的结构特点，运用I-DEAS软件建立基于接触的L-B型制动梁有限元模型，并计算了制动梁在不同工况下的应力分布，计算结果表明应力集中主要发生在闸瓦托滑块根部，这与实际运行中出现的故障较为吻合，为制动梁的改进设计和结构优化提供了依据。

Methods 100 examples,randoms choosing scheduled time line underbelly operation (operation,the womb cut completely or satisfy time eager operation,ovary tumour operation including cutting the palace product open) divide into two set,a forms:give hard film outside rentaini 0.4 mg + 0.75% bupivacaine 20 ml formulating cheng 100 ml analgesias,b group behind the skill:100 ml compounding cheng ease pain,comparatively,the life body collects,optesthesia simulates grade and bad reaction in giving hard film outside rentaini 0.4 mg + interest rate of 0.75% bupivacaine 20 ml + fluorines pai many 2 ml after the skill.results two set of patient skill queens circulation stabilizes equally,skill queen vas difference has no statistics significance,two set of analgesic effects have no notable difference,b many by-reaction of fluorine pai benefit appears on group but.

择期行下腹部手术(包括剖宫产手术、子宫全切或次全切手术、卵巢肿瘤手术)100例，随机分成两组，a组：在术后予硬膜外芬太尼0.4mg+0.75%布比卡因20 ml配制成100 ml镇痛。b组：在术后予硬膜外芬太尼0.4mg+0.75%布比卡因20ml+氟哌利多5mg配制成100ml镇痛，比较生命体征、视觉模拟评分和不良反应。结果两组患者术后循环均稳定，术后vas差异无统计学意义，两组镇痛效果无显著差异，但b组出现氟哌利多不良反应。

The highest content of HCB is 6,254 times higher than the B level standard set by the Ministry of Environmental Protection, carbon trichloride 9,330 times higher, hexachlorobutadiene 290.5 times higher.

六氯苯最高超出环保部B级标准6254倍，六氯乙烷最高超出环保部B级标准9330倍，六氯丁二烯最高超出环保部B级标准290.4倍。

In OWE-SOJ test, a fluffy colony without clampconnection formed in the A=B≠mating interaction where nuclear migration occurs, whilea barrage colony without clamp connection formed in A≠B= interaction where nuclearmigration do not occur. The two colonies could be distinguished clearly from each other.

而在OWE-SOJ试验中，发生核迁移的A=B≠交配反应形成无锁状联合的绒毛状菌落，而不发生核迁移的A≠B=反应形成无锁状联合的栅栏型菌落，两种菌落可以清楚地区别开来。

In OWE-SOJ test, a fluffy colony without clamp connection formed in the A=B≠ mating interaction where nuclear migration occurs, while a barrage colony without clamp connection formed in A≠B= interaction where nuclear migration do not occur. The two colonies could be distinguished clearly each other.

而在OWE-SOJ试验中，发生核迁移的A＝B≠交配反应形成无锁状联合的绒毛状菌落，而不发生核迁移的A≠B＝反应形成无锁状联合的栅栏型菌落，两种菌落可以清楚地区别开来。

Methods: Eighteen SD rats were randomly divided into A,B,C groups. A,B groups were induced to RE by operation, C group had false abdominal operation. All rats were killed 2-3 months later and the NO,VIP neurons were investigated qualitatively and quantitatively by immunohistochemical method and color image analyzer.

18只SD大鼠随机分为A、B、C3组，A、B两组手术造成RE，C组采用伪开腹术。2～3个月时用免疫组化法及彩色图像分析仪对3组大鼠食管组织中的NO及VIP含量进行定性、定量测量。

In recent years, a new county health agencies work space 33000 square meters, adding a large number of medical instruments and equipment, county-level medical institutions equipped with CT, large-scale full-automatic biochemical analyzer, color B Ploymetric advanced equipment, township hospitals equipped with X -ray machine, B super-machines and laboratory equipment.

近年来全县医疗卫生机构新建工作用房33000平方米，增添医疗仪器设备一大批，县级医疗机构配备了CT、大型全自动生化仪、彩色B超等先进仪器设备，乡镇卫生院配备了X光机、B超机和化验设备。

We can also be the B-, color finger when the doctor said it can be seen clearly, but I also secretly watch are a boy or a girl, so many times to do B-.

我们这的B超还可以，彩超时医生说手指头都可以看得清楚呢，而且我还偷偷去看是男孩还是女孩，所以做了很多次B超。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。