查询词典 Aspergillus

objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.

目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心elisa法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示，用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株，而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

Methods BALB/c mice were immunized by cytoderm antigen of Aspergillus fumigatus, excreting antigen and inactivated conidiospore respectively, to prepare monoclonal antibodies specific to Aspergillus. Cross-reactions between mAbs and antigens from Aspergillus or Monilia genus were characterized by immunofluorescence.

采用烟曲霉细胞壁抗原、分泌抗原和灭活分生孢子，分别免疫BALB/c小鼠，制备特异性的抗曲霉单克隆抗体，免疫荧光法鉴定单克隆抗体与曲霉属和念珠属抗原的交叉反应。

A strain library containing 226 bacterial and fungal xylanase producers was successfully constructed, two bacterial producers Pseudomonas sp WXULI-11 and WL-11 and three fungal producers Aspergillus niger XY04B, Aspergillus niger XY07C and Aspergillus glaucus XY12D were screened out from this library.

建立了一个包括226株细菌和霉菌木聚糖酶产生菌的小型菌种库，从中筛选出了2株细菌（Pseudomonas sp WXULI-11和WL-11）及3株霉菌（Aspergillus nigerXY04B、Aspergillus niger XY07C及Aspergillus glaucus XY12D），它们的共同点是木聚糖酶生物合成量高，且不产或少产纤维素酶。

Results Observation of 35 746 grain of airborne fungi spores and 1 188 grain of fungi spores for one year revealed the following species of fungi: Ustilaginales, Aternaria, Uredinales, Clodoporium cladosporioides, Cladosporium, Pithomyces, Cladosporium macrocarpum, Aspergillus niger, Aspergillus versicolor and Aspergillus fumigatus. Thirth-nine of 85 infants and young children with asthma (45.9%) showed positive skin test and 108 of 260 older children with asthma (41.5%) were positive for the skin tests; total positive rate was 42.61%.

结果 经1年的调查，曝片观察到的35 746个真菌孢子和曝皿观察到的1 188个菌落，分属于4个亚门60个种属，常见菌种有黑粉菌、交链孢菌、锈菌、枝孢芽枝菌、芽枝菌、皮思菌、大孢枝孢菌、黑曲菌、杂曲菌和烟曲菌。85例婴幼儿哮喘患儿，39例真菌变应原皮内试验阳性（45.9％），260例儿童哮喘患儿，108例皮试阳性（41.5％），总阳性率42.6％。

Based on macromorphological and micromorphological characters, as well as conidium ornamentation observed with scanning electron microscopy, Aspergillus sp. D-l was affiliated to Aspergillus Section Versicolores. Further, DNA sequence of the internal transcribed spacer was used as the molecular characteristic, and analyzed with the DNAMAN 5.2.2 program and the Mega 3.0 program.

采用真菌核糖体基因内转录间隔区序列作为分子标记，利用DNAMAN 5.2.2和Mega 3.0等软件对其进行同源性和进化关系分析，在种的水平上将Aspergillus sp.D-1归属为Aspergillus versicolor，并将该菌株命名为A.versicolor D-1。

The results show that the dispersity of emulsion is good and the average diameter of particles is about 15 nm. Its bactericidal properties to some bacteria and epiphytes are strong under daylight lamp irradiation. The killing rate to Gram negative bacterium (Staphylococcus aureus and Bacillus subtilis) is 100%, in addition, killing activity of it to Gram positive bacterium is very high. It has a clear antibacterial circle of about 20 mm in diameter toward epiphytes such as Aspergillus niger, Aspergillus flavus and Candida albicans etc.

结果表明，混晶乳液分散性好，粒径约15nm；在普通日光灯照射下，乳液对一些细菌和真菌有很好的抗菌性能：对革兰氏阳性菌（金黄葡萄球菌和枯草芽孢杆菌）的杀灭率达到100%，对革兰氏阴性菌也有很好的杀灭性能，对黑曲霉、黄曲霉和白色念珠菌等真菌产生清晰的抑菌圈，抑菌直径约20mm。

At the same time,the antagonism were confirmed by filter paper method.According to the classification standard of Raper(1965),NO.1 strain is generally in good agreement with the standard of holotype Aspergillus niger V.Tieghen 1867,therefore,we identified and named the type culture as Aspergillus niger xj.

Tieghen 1867的标准形态基本相同，鉴定该菌株属于曲霉属黑曲霉群的一株黑曲霉，记为黑曲霉xj，并将该菌种保藏在中国典型培养物保藏中心。

Mold of 34 species were isolated from moldy books.Among them,3 species which were demonstrated to be the key mould causing book mold were identified morphologically and molecularly as Chaetomium globosum,Aspergillus terreus and Aspergillus fumigatus.

从霉变的书籍中分离纯化了34种霉菌，对其中的3种主要霉菌进行了形态学和分子生物学鉴定，分别鉴定为球毛壳菌种、土曲霉种、烟曲霉种。

At present, most of lactases used in industry production come from Kluyveromyces, Aspergillus niger and Aspergillus oryzae.

目前，工业生产中使用的乳糖酶主要来源于乳酸克鲁维斯酵母菌、黑曲霉和米曲霉。

It is significant that aspergillus be supervised using PCR in preventing and controlling of aspergillus infection in organ transplant recipients

应用PCR方法对器官移植患者进行曲霉菌监测，为预防和控制由曲霉菌感染造成患者死亡具有重要的意义。

Now she was hungry and angry.She began to smoulder.

现在她又饿又气，她开始流露难以抑制的怒火。

You have placed our iniquities before You, Our secret sins in the light of Your presence.

诗90：8 你将我们的罪孽摆在你面前、将我们的隐恶摆在你面光之中。