查询词典 Annulata

To evaluate the specificity of the PCR, genomic DNA of Theileria annulata,Babesia bovis,Toxoplasma gondii,Leishmania donovani and standard strain of N. caninum were used as a template in the PCR. For determining the detection limit of amplification procedure, PCR was run on a dilution series of genomic DNA from N. caninum（1.562 5-200 ng/ml）. Brain tissue samples of 32 aborted fetuses were detected by PCR-based assay, and 23 blood samples from mothers were tested by ELISA. Results The amplified DNA fragment （350 bp）had a high identity of 98% with the Nc-5 gene sequence of N.

以环形泰勒虫、牛巴贝斯虫、刚地弓形虫、杜氏利什曼原虫以及犬新孢子虫标准株DNA为模板进行扩增以验证PCR的特异性，采用紫外分光光度计测定犬新孢子虫标准株DNA浓度和纯度，取高纯度的DNA样品用灭菌水稀释，分别取不同量的DNA进行PCR扩增，确定PCR方法的敏感性；利用该方法对32份奶牛流产的胎牛脑组织样品进行检测，同时，对其中23份流产的母牛血样进行ELISA血清学检测，以评价犬新孢子虫PCR方法的检测效果。

Getting blood from vena cervicalis, making blood smear as routine, Giemsa staining were used to study through immersion objective observation on naturally case in clinic. The result indicated that many red blood cells infected T. Annulata in number from one to three, which infected one polypide take up the most and two the less, while that of three polypides take up the least.

对临床自然病例进行颈静脉采血，按常规制作血液涂片，Giemsa氏染色，油镜下观察和研究，结果表明：许多红细胞感染有1~3个数量不等的环形泰勒虫，以1个的为最多，含有2个虫体的红细胞较少，偶尔可见到含有3个虫体的红细胞。

According to the Tams1 gene of bovine Theileria annulata published on GenBank, two pairs of primers were successfully designed to develop nested PCR assay for detecting the disease caused by bovine Theileria annulata.

根据GenBank上发表的环形泰勒虫Tams1基因序列设计2对巢式引物，成功建立了环形泰勒虫病的巢式PCR快速检测方法。

In order to analyze gene sequence of the bovine Theileria annulata in Xinjiang, a pair of primers was designed, Tamsl gene fragment was amplified by PCR, sequence of positive clones was showed that the gene fragment has a total length of 846 bp, which encoded 281 amine acids.

采用PCR技术，从新疆焦虫病疫区感染牛的全血中扩增到Tamsl基因，该基因长度为846 bp，编码281个氨基酸。

The subsequent larval and nymphal progeny were applied onto the calves infected with Theileria annulata in order to investigate whether Hy. a. anatolicum were infected by both Theileria annulata

结果显示：巴贝斯虫未定种首先在蟀的肠道内发育，经一系列未知的变化后，在蟀饱血脱落5天内形 2 摘要成运动体，随后，几乎同时可在肠道、血淋巴、唾液腺及卵巢内发现大裂殖体

Objective: To study the effect of different single C-sources, N-sources and C/N ratio on the submerged-culture of Stropharia rugoso-annulata Methods: Single factor and orthogonal testing method were employed for this study.

目的：以菌丝生物量为指标，探讨不同碳氮源及碳氮比对大球盖菇液体发酵的影响。方法：单因子试验和正交试验。

The necessity of these environmental problems calls for the utmost prudence to treat agricultural intensification. Based on detailed expatiation of intensive agriculture, this case study was carried out in the town of Shipan of Jianyang Municipality where a demonstration of rotation of edible mushroom Stropharia rugoso-annulata and rice.

本文对集约化农业作了详细的阐述，在此基础上，以简阳石盘镇水稻和大球盖菇轮作方式为例，该地作为降解作物秸秆的大球盖菇的试种点，改变了以往种植常规作物的农业方式，使大量作物秸秆还田用于生产食用菌大球盖菇。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷