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This experiment with dilute alkali swelling on collagen extraction and separation, then purified by gel from collagen, and then glutaraldehyde, carbodiimide, has three types of di-isocyanate cross-linking agent on the plastic recombinant protein molecules cross-linked, the final test of infrared spectroscopy analysis of the results of their cross-linking to determine the extent of the effectiveness of cross-linking cross-linking agent to have an initial understanding.

本实验用稀碱溶胀法对胶原蛋白进行提取与分离，接下来用凝胶层析纯化所得胶原蛋白，然后用戊二醛、碳化二亚胺、己二异氰酸酯三种交联剂对胶原蛋白分子进行重组交联，最后通过对红外光谱测试结果的分析，对其交联程度进行判断，对交联剂的交联功效有一个初步的了解。，谢谢。

A method for eliminating the internal oxide structure in the gas-phase carbon-nitrogen osmosis of gear features that the CCL4 is added to the kensene as carburizer and then is decomposed to generate Cl2 for reducing the oxide, the ammonia gas as nitrizing agen passes through the cylinder of silica gel or granular calcium chloride for adsorbing residual water vapour and then through the cylinder of 5A molecular sieve for adsorbing residual O2, and the resistance wire is wound on igniting pipe at furnace top to prevent the NH4Cl in exhausted gas from being condensed.

本方法采用在气体渗碳剂煤油中，加入6～8％的CCl 4 ，使共渗炉内的CCl 4 分解出新生的Cl 2 ，使已形成的合金元素内氧化物还原；在渗碳剂氨气入共渗炉前，先经过硅胶瓶或桶及粒状氯化钙瓶或桶过滤，吸附掉氨气中的残留水蒸汽，再经过5A分子筛瓶或桶吸附掉氨气中的残留氧；在靠近炉顶位置的点火管周围缠绕带瓷环的电阻丝，靠电阻丝的副带加热，使排气中的NH 4 Cl不冷凝结块等措施实现内氧化组织的消除。

The biological N-fixation rate of Chamaecrista rotundifolia showed a trend of increasing and then decreasing, climbing to the peak of 29.50% at the N2 level (30 mg/kg) and then gradually decreasing, had a highly significant difference between the treatments. At a certain N level, the N absorption of C.

结果表明：圆叶决明生物固氮百分率随着施氮水平的增加呈先升高后下降的趋势，并在N2（30 mg/kg）水平下达到最高，为29.50％，之后逐渐下降，且各处理间差异极显著。

There are two synthetic methods for the two carboxylates starting from pyrrole,(1) the carbonylation of pyrrole using trichloroacetyl chloride and then the esterification reaction in methanol, or 2-MPC can be directly synthesized from pyrrole and methyl chloroformate,(2) the insertation of CO2 to pyrrole for the corresponding 1-pyrrole carboxylic acid or 2-pyrrole carboxylic acid and then the esterification reaction in methanol.

两种吡咯甲酸甲酯的合成从吡咯为起始原料有两种方法：（1）使用酰氯将吡咯酰化，然后与甲醇完成酯化反应，或者吡咯与氯甲酸甲酯直接合成吡咯甲酸甲酯；（2）先将吡咯通过CO2插入反应得到1-吡咯甲酸或2-吡咯甲酸，然后与甲醇反应完成酯化。

The main conclusions are as follows:The vitellin of Chrysopa septempunctata was extracted with 0.4mol/L NaCl and then precipitated in cold distilled water. Ion exchange chromatography and proteic gel recovery were used to purify it further. SDS-PAGE analysis shows that the vitellin is composed of two subunits, the large one 165kDa and the small one 46kDa.Then automatic amino acid analyzer was used to analyse amino acid composition of vitellin.

主要结论如下：用蒸馏水沉淀法对大草蛉的卵黄蛋白进行了初步纯化，而后又采用离子交换层析和蛋白胶回收的方法对卵黄蛋白进行了进一步的纯化；通过SDS-PAGE分析表明，大草蛉的卵黄蛋白由大165kDa和小46kDa两个亚基组成；用氨基酸自动分析仪对纯化后的卵黄蛋白进行了氨基酸组分分析。

With substitution degree added, the colocasia esculenta schott starch phosphate ester was easier to gelatinize, the tolerance to salt and sucrose increased, the clarity first increased and then decreased, paste viscosity first decreased and then increased.

随着取代度的增加，香芋淀粉磷酸酯的糊化变得容易，耐盐和耐糖能力提高、透明度先增后减、糊粘度则先减后增。

The invention uses the method of the fusion and direct polymerization to prepare the carboxyl-terminated lactic acid prepolymer and then copolymerize with the aliphatic polycarbonate; then the reaction is done in the twin-screw extruder to screw out the extension chain and the good polylactic acid copolymer is made.

本发明采用熔融直接缩聚的方法制备出羧基封端的乳酸预聚物，接着再与脂肪族聚碳酸酯二元醇进行共聚，然后再在双螺杆挤出机中进行反应挤出扩链，制备性能良好的聚乳酸共聚物。

I drove over the mountains from Dasi to Fusing, Cilan to Yilan and then on FWY 5 to FWY3 then to Keelung and Yeliou to see the rock structures.

我驱车翻山越岭从大溪到复兴，Cilan到宜兰然后从5号高速公路到3号然后到了基隆野柳，看了女王头。

Well,in the past i watched recs of all the top experts,especially from chris daut and ruso,they are the strongest players in my eyes in the past ,then i reached 1k8 i had a trainer who helped me improving ,we played like 20-30 games a day arabia only huns war,i finally was able to reach 2k and then i started to play fr only ,ara is perfect training for speed micro and fr is perfect training for map understanding,i always tried to play vs better players

好的，在过去我经常看顶级高手的录象，特别是来自CHRIS DAUT和RUSO的录象，在过去他们在我的眼里简直就是无敌。当我达到1800+的时候我有一个教练帮助我提高，光在阿拉伯地图就用匈奴我们经常每天打20到30局，当我接近2000+的时候我就开始玩随即地图，最后我在阿拉伯地图上和完全随即地图上我可以做到用最少的时间做出最完美的操作。我总是和比我好的玩家打局。

The sperm whales in Odontoceti was closely related with baleen whales in Mysticeti, and then clustered with Delphinoidea and then with Ziphioidea.

其中抹香鲸总科与须鲸亚目聚合在一起，然后与海豚总科聚合再与喙鲸总科相聚。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。