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The omp17.3 gene of Brucella abortus was amplified by PCR and then the amplicon was cloned into the eukaryotic expression plasmid pcDNA3.1 to construct a recombinant plasmid pcDNA3.1-omp17.3. Then the recombinant plasmid pcDNA3.1-omp17.3 was transfected into COS7 cells, and the expressed OMP 17.3 was detected by Western-blotting.

采用PCR方法扩增了布鲁氏菌17.3ku外膜蛋白编码基因，并将该基因克隆至真核表达载体pcDNA3.1中，成功构建了真核表达质粒pcDNA3.1-omp17.3.pcDNA3.1-omp17.3转染COS-7细胞后，通过Western-blotting检测到了17.3ku蛋白的瞬时表达。

After cleaning in the morning and at night, then use absorbent cotton to rub it on your skin from inner to outer by following up the skin texture, and then pat your skin gently by fingers to improve the absorption effect.

早晚清洁后用浸湿的脱脂棉按肌肤纹理从内到外涂抹，然后用手指肚轻轻拍打以提高吸收效果。毁容式洗脸法你还。。。女人夏季保湿易被。。。

First of all, make sure that your machine has been installed above VC6.0 to compile the executable file to generate the need to open the dsw file name suffix, the system will default to open with VC, and then select the Build menu, Set Active Configuration under the option, Project Configuration options for the Win32 Release, and then compile the project, the executable file.

首先确认您的机器上已经安装了VC6.0以上版本，要编译生成可执行文件，需打开后缀名为dsw的文件，系统会默认用VC打开，然后选择Build菜单下的Set Active Configuration选项，选择Project Configuration为Win32 Release，然后编译项目，得到可执行文件。

It is a good idea, and then fresh proposals, and then plan and left the communication with its staff are unable to implement the castle in the air.

再好的想法，再有创见的建议，再完善的计划，离开了与员工的沟通都是无法实现的空中楼阁。

I'll seize you in a precise grip, then I'll devour you and digest you, and then, using the talismanic power of my dialectal research, I'll distill a potent brew in my alembic.

我要准准地抓住你，吃掉你，消化掉你，然后我使出我的方言调查的法宝，也来炮制一下。

Software Description: XICalc is an integer calculator program with the following features: multiple precision integers with millions of digits; uses Hartley Transform to speed-up long multiplies; separate input and outputnotation, base 2 to base 36; greatest common divisor function; Modulo arithmetic options; Bernoullinumbers, Euler Numbers; Boolean functions; random number generator; prime factor algorithm using theElliptic Curve Method; number theoretic functions phi, sigma and tau; prime testing and searching using the Adleman function; algebraic notation; if-then-else statement; go-to statement; can run XICalc code from files; hot help from F1 key; and it抯 written in Microsoft Visual C#.

XICalc 是一款带有如下功能的综合性计算器软件：带有百万数位的多精度整数；使用 Hartley Transform 加速长整型多样化计算；以2到36为基数分隔输入和输出符号；最大公约数函数；模数算法选项；Bernoulli 数字；Euler 数字；布尔函数；随机数字生成器；使用圆周曲线算法的主要因素运算法则；π，τ，Σ的数字理论函数；使用 Adleman 函数的最初测试和搜索功能；代数符号；if-then-else 语法结构声明；go-to 语法结构声明；来自于文件的XICalc 代码；F1键的热点帮助；并且该软件是采用适用于 Windows 操作系统的微软 Visual C#。

The second part, this paper relaxed the supposition of noncooperation, supposing the relationship of franchisor and franchisee is cooperative, and then test whether the franchisor and franchisee can improve the payment schemes gained from the first part through cooperating, in this part, we used the theory of Parreto efficient, and the results showed that, the MBR contract way has reached the optimal point of Parreto, while the SBR contract way hasn't, which can be mended through cooperating, and then increasing the profits of each other farther.

在研究的过程中，本文运用了帕累托有效性理论，结果显示：MBR 的合约安排已达到帕累托最优，不存在可以改进的地方；而SBR 的合约安排则未达到，特许双方可以通过合作来进一步提高他们的利润。

For a few seconds afterwards I have a fine spiritual glow… and maybe it would continue that way indefinitely – how can you tell?– if it weren't for the fact that there's a woman beside you and then the douche bag and the water running… all those little details that make you desperately selfconscious, desperately lonely. And for that one moment of freedom you have to listen to all that love crap… it drives me nuts sometimes… I want to kick them out immediately… I do now and then.

完事以后有几秒钟我觉得精神振奋……也许这种精神状态会无限期地持续下去-若不是身边有个女人，还有装灌洗器的袋子，水在哗哗流……这些微小的细节使得你心里清楚得要命，使你觉得十分孤独，而就在这完全解脱的一瞬间内你还得听那些谈论爱情的废话……有时这简直要叫我发疯……我不时发疯。

Then the duke he lets the curtain down, and bows to the people, and says the great tragedy will be performed only two nights more, on accounts of pressing London engagements, where the seats is all sold already for it in Drury Lane; and then he makes them another bow, and says if he has succeeded in pleasing them and instructing them, he will be deeply obleeged if they will mention it to their friends and get them to come and see it.

啊，看这个老傻瓜的这番精彩演出，恐怕连一头牛也会哈哈大笑吧。接下来公爵拉下大幕，对观众一鞠躬，说这场伟大悲剧只能再演两个晚上，因为伦敦方面有约在先，在特勒雷巷戏院里的座位早已预订一空。

The litany begins with the call for mercy upon God the Father, God the Son, and God the Holy Ghost, in the "Kyrie eleison","Christe eleison", Kyrie eleison. Then, considering Christ as our Saviour and Mediator, we ask Him to hear us. In order to render more secure the hearing of our prayers, we again ask each of the Persons of the Holy Trinity for mercy, and, adding those titles which give us a claim to Their consideration, we call upon the First Person: God, the Father of Heaven, to whom we owe existence and life; the Second: Redeemer of the world, to Whom we owe our salvation; the Third: Holy Ghost, to whom we owe our sanctification; and then on the Holy Trinity, one God.

该一长串开始呼吁怜悯的上帝，父亲，上帝的儿子，和上帝圣灵，在& kyrie eleison &，& christe eleison &， kyrie eleison ，然后考虑以基督为我们的救主和调停，我们问他听到我们的，以使更安全的听证会我们的祈祷，我们再次要求每个人的圣三一为怜悯，并加入这些书籍，这给我们一个声称他们的考虑，我们呼吁第一人：上帝，父亲的天堂，向谁，我们有责任的存在和生活；第二：救赎的世界，向谁，我们有责任我们的拯救；第三：圣灵，向谁，我们有责任，我们成圣；然后就圣公会圣三一，一上帝。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。