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Firstly, this article introduces the development of Embedded System and Dynamic Electrocardiogram. The work's situation, studying purpose, contents and configuration are discussed. Then, by analysis of Electrocardiogram, the traits of Holter is introduced, and then bring up the design idea of the work's software and hardware, and the work's design and engineering materializing are detail discussed.

首先，本文介绍了嵌入式系统和动态心电监护技术的发展和现状，指出现存的问题和研究意义，论述了课题的主要研究内容；然后，在对心电信号介绍的基础之上，论述便携式心电记录仪系统设计的性能指标，提出了该心电记录仪软硬件系统的设计思想，详细阐述了各部分设计思路和工程实现。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分为4个部分，第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究，确立了一种较好的培养方法：与颗粒细胞共培养，找到了一种适合猪卵母细胞体外成熟的培养基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸，成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%，国外报道的最高成熟率为(85.7±4.1)%；第二部分对猪卵母细胞孤雌激活的化学方法进行了研究，确立了化学激活猪卵母细胞的两种最佳方法：1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min，再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中，卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min，再与8mM的DTT共孵育30min，卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%；第三部分对孤雌激活胚胎的培养条件进行了研究，确立了一种最佳的胚胎培养条件：在SOF简单培养基中添加颗粒细胞进行前3天的培养，然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养，其桑椹胚和囊胚的发育率为(59.5±3.2)%；第四部分研究了IGF-I

That the patient was found, we will, through this epidemic, will soon go out of the newspaper, the CDC and then the patient will be carried out epidemiological investigation, and then to his home, and his close contacts and so on, To carry out the investigation and handling of the epidemic, this is a very rigorous process, and the second is that we have a comprehensive prevention and control measures, have done a lot of work, the most famous is the patriotic public health campaign, which cut off for Route of transmission, has played a powerful role in the promotion.

那这个病人被发现以后，我们就会通过我们这个疫情，很快的报出去，然后疾控中心就会对病人进行流行病的调查，然后对他的居所，还有他的密切接触等等，要进行流行病的调查和处理，这是一个非常严格的一个过程，第二个，就是我们在一个综合的防控措施方面，做了大量的工作，最著名的就是爱国卫生运动，这个对于切断传播途径，起到了很有力的促进作用。

The weight percentage for the components of the hyaline coating is as follows: SiO2 45 to 54 percent, B2O3 15 to 20 percent, one or more of Na2O, K2O and Li2O, 10 to 24 percent, one or more of TiO2, ZrO2 and ZnO, 6 to 18 percent , and the rest is one or more of CaO, MgO and CeO2. Firstly, the glass powder is mixed with the mill additions to be pulped and then plated on the surface of titanium or the titanium alloy. After being dried, the surface of titanium or the titanium alloy is directly used for a thermal hydrogen treatment or smelted at a temperature of 900 to 1, 000 DEG C for 15 to 30 minutes and then a hyaline coating with a thickness of 20 to 70 micrometers is formed on the surface of titanium or the titanium alloy; secondly, titanium or the titanium alloy plated with the coating is conducted a thermal hydrogen treatment. After the treatment technique is finished, the coating flakes off automatically in the process of cooling; and finally, a surface treatment is supplemented to meet the requirement of the workpiece surface.

该玻璃质涂层成分按重量百分比：45-54SiO2，15-20B2O3，10-24Na2O、K2O、Li2O中的一种或多种，6-18TiO2、ZrO2、ZnO中的一种或多种，余量CaO、MgO、CeO2一种或多种，将玻璃粉与磨加物混合制浆并涂覆在钛或钛合金表面，干燥后直接用于热氢处理，或在900-1000℃下熔烧15-30min，在钛或钛合金表面制得20-70微米玻璃质涂层，随后将制有该涂层的钛或钛合金置于氢气气氛下进行热氢处理，处理工艺结束后，涂层在冷却过程中自剥落，最后辅以表面处理即可满足工件表面要求。

Results show that microbial biomass carbon and microbial biomass nitrogen in fertilized soil peak in the first several days, and then decline. Incubating both sweet maize straw and white clover have the highest yield, maxima of 529.57 mgkg^(-1) and 75.50 mgkg^(-1) respectively. They also release respired CO2 the most among all treated soils. The best mineral nitrogen release is in white clover incubating, with a maximum of 29.81 mgkg^(-1) under a 26-day culture, and then keeping 1.60 times the control and reaching 2.48 times at 80-day culture. Sweet maize incubating has the least mineral nitrogen release. It is 13%~53% of the control under a 26-day culture, with a maximum of 7.51 mg kg^(-1). Soil nitrogen mineralization is insignificant under sweet maize incubating with urea in short-term.

研究发现，各施肥处理的土壤微生物量碳、微生物量氮均在培养前期出现峰值，后期平稳降低；甜玉米桔秆和白三叶草绿肥同时还田的土壤微生物量碳、微生物量氮在各培养时期均最大，峰值分别达529.57 mgkg^(-1)，和75.50 mgkg^(-1)，土壤呼吸产生的CO2最多；白三叶草绿肥单独还田有利于土壤无机氮的释放，培养第26d无机氮达到最大值，为29.81 mgkg^(-1)，之后一直在对照处理的1.60倍以上，第80d达到2.48倍；甜玉米秸秆单独还田不利于土壤无机氮的释放，培养的第26d至结束，甜玉米秸秆处理的无机氮为对照的13%~53%，最大为751 mgkg^(-1)；甜玉米秸秆配施尿素，短期内不利于土壤无机氮矿化。

Results show that microbial biomass carbon and microbial biomass nitrogen in fertilized soil peak in the first several days, and then decline. Intercropping Incubating both sweet maize straw and white clover have the highest yield, maxima of 529.57 mg·kg-1 and 75.50 mg·kg-1 respectively. They also release the most respired CO2 the most among all treated soils. The best mineral nitrogen release is in white clover intercroppingincubating, with a maximum of 29.81 mg·kg-1 under a 26-day culture, and then keeping 1.60 times the control and reaching 2.48 times at 80-day culture. Sweet maize incubatingintercropping has the least mineral nitrogen release. It is 13%~53% of the control under a 26-day culture, with a maximum of 7.51 mg·kg-1. Soil nitrogen mineralizatiomineralization is insignificant under sweet maize incubatingintercropping with urea in short-term.

研究发现，各施肥处理的土壤微生物量碳、微生物量氮均在培养前期出现峰值，后期平稳降低；甜玉米秸秆和白三叶草绿肥同时还田的土壤微生物量碳、微生物量氮在各培养时期均最大，峰值分别达529.57 mg·kg-1和75.50 mg·kg-1，土壤呼吸产生的CO2最多；白三叶草绿肥单独还田有利于土壤无机氮的释放，培养第26 d 无机氮达到最大值，为29.81 mg·kg-1，之后一直在对照处理的1.60倍以上，第80 d达到2.48倍；甜玉米秸秆单独还田不利于土壤无机氮的释放，培养的第26 d至结束，甜玉米秸秆处理的无机氮为对照的13%～53%，最大为7.51 mg·kg-1；甜玉米秸秆配施尿素，短期内不利于土壤无机氮矿化。

Has included filling of 3000 vials and stoppering on line; another stage included filling 3000 vials, transportation to the lyophilizer and then stoppering; a third stage included the filling of 3000 vials, loading in the lyophilizer, and exposure to a portion of the nitrogen flush and then stoppering.Since lyophilizer sterilization and sterilization of the nitrogen system used to backfill require separate validation, media fills should primarily validate the filling, transportation and loading aseptic operations.

第一部分是灌装3000瓶并且加塞，第二部分是灌装3000瓶，传递到冻干机中再全加塞，第三部分是灌装3000瓶，排放到冻干机内，暴露在氮气流下冻干机的灭菌和用于回填的无菌氮气系统分别进行验证，培养基灌装主要验证灌装、传递和摆放的无菌操作。

First, rebuilt the concept of Cellular Consciousness of Marxism philosophy , and then putted forward the concept of Cellular Consciousness by analyze and sum-up to live cellular being activities. Secondly, expatiated on the evidence of biology and idiographic effect that the Cellular Consciousness is existence, and then demonstrated syllabifies the connection between the Cellular Consciousness and the Original Cellular Consciousness.

首先对马克思主义哲学的意识概念给予重新认识，再通过对细胞在其生命时期的诸多生命活动进行归纳和总结，从而提出细胞意识的概念；又通过详细地阐述细胞意识存在于单细胞生物及多细胞生物体内的生物学依据及其所发挥的具体作用，从而清晰地论证细胞意识与原始意识之间的相互关系，以详尽地指出细胞意识存在的巨大意义。

Experiments on the effects of flood for agricultural crops were done in Zhumadian. corn was selected as the experimental material, and choosing 3 days, 5 days and 7 days submerged days and the jointing stage, tasselling stage for artificial irrigation respectively. The field was given conventional management. After harvest, we recorded various characters and production data, and then compared them with the controlled areas. Then analysis and assessment of the impact of main flood factors on the crops were built.

2在驻马店市驿城区申庄村进行农作物洪水影响田间试验，用玉米作为试验材料，选取3 d、5d、7d三个淹没天数和拔节期、抽雄期两个淹没阶段分别对其进行人工灌水，田间采取常规管理，在作物收获后对玉米的各个性状及产量数据做记录并和对照区进行对比，进而分析和评估洪水灾害发生时对农作物的影响情况。

The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2／6／7病毒样颗粒，待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解，培养基上清和细胞裂解液样品两次40％蔗糖垫93000g超速离心，沉淀样品经SDS-PAGE胶分离，用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性，电镜检测重组病毒样颗粒的形态结构。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。