查询词典 And Then

Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml，分离单个核细胞体外诱导培养DC，并将实验组和对照组细胞各分为三组，A组为未处理组，B组为冻融组，C组为RNA转染组。A组DC在培养5d后，每孔加入TNF-α200U/ml；B组DC培养第5d后，每孔中加入癌细胞蛋白抗原，使DC/肿瘤细胞=1：1，继续培养4h后，每孔加入TNF-α200U/ml；C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC：RNA=1×105/ml： 5ug，按照invitrogen DMRIE-C试剂说明书进行操作)，转染结束后用完全培基取代转染剂，每孔加入TNF-α200U/ml。

The glass and crystalline glass-solders of Li2O-ZnO-SiO2 system were prepared by conventional melt technology. The various factors effecting on glass ceramics and glass were investigated,which include glass chemical composition and heat-treatment and so on. And then by means of XRD, DTA, IR and SEM, the structure and properties of the glass and glass-ceramic were studied. The coefficient of linear expansion , transform temperature and soft temperature wer measured. And then the factors that effect the mechanical strength of glass-ceramic were discussed and the fluxion property was determined and the crystallization course and mechanism were discussed, which can provide certain therial reference value for practice producing and glass seals to glass, ceramics and metal.

用高温熔融法制备了Li_2O-ZnO-SiO_2系统玻璃及其结晶型玻璃焊料，探讨了各种因素(包括玻璃组成、热处理制度等)对微晶玻璃的结构和性能的影响；利用X射线衍射分析、DTA、红外光谱分析以及扫描电镜等现代测试手段，对玻璃的结构与性能进行研究，测定了玻璃及微晶玻璃的线膨胀系数、转变温度和软化温度，同时对影响微晶玻璃的强度的因素进行了探讨，并对该体系玻璃的流散性进行了测定，对它的析晶过程和析晶机理进行探讨，为实际生产和与玻璃、陶瓷及金属封接提供了一定的理论参考价值。

The first thing I proposed to myself was, to throwdown my enclosures, and turn all my tame cattle wild into thewoods, lest the enemy should find them, and then frequent theisland in prospect of the same or the like booty: then the simplething of digging up my two corn-fields, lest they should find sucha grain there, and still be prompted to frequent the island: thento demolish my bower and tent, that they might not see any vestigesof habitation, and be prompted to look farther, in order to findout the persons inhabiting.

这就是说，他们只吃战争中所抓到的俘虏，平时一般是不吃人的。此后过了很久，有一天，天气晴朗，我和星期五偶然走上岛东边的那座小山顶。在那儿，也是在一个晴朗的日子里，我曾看到了美洲大陆。当时，星期五全神贯注地朝大陆方向眺望了一会儿，忽然出乎意外地手舞足蹈起来，还把我叫了过去，因为我恰好不在他身边，离开他还有几步路。我问他是怎么回事。他说：&噢，真高兴！真快活！我看到了我的家乡，我看到了自己的部落了！&

The invention relates to a pipeline polyurea outer anticorrosive coating and the making method thereof, making heated prepolymerization reaction on dibenzo-methane diisocyanate, carbodiimide diisocyanate, polyether binary alcohol and polyether trihydric alcohol to prepare prepolymer solution A, and making end amino polyethylene oxide ether, dimethyl sulfur-based toluene diamine, diethyl diamine, iso-propyl titanate solution, polyacrylate, carbon black, titanium dioxide and lightweight calcium carbonate into solution B, and as using, respectively heating the solutions A and B to 65 deg.C and then mixing them, then quickly spraying and curing to form a coat.

本发明涉及一种管道用聚脲外防腐涂料及制造方法，由二苯基甲烷二异氰酸酯、碳化二亚胺化二异氰酸酯、聚醚二元醇和聚醚三元醇通过加热预聚反应制成预聚物的A液、由端氨基聚氧化乙烯醚、二甲硫基甲苯二胺、二乙基甲苯二胺、钛酸异丙酯溶液、聚丙烯酸酯溶液、碳黑、钛白粉和轻质碳酸钙配制而成的B液组成，使用时将A液和B液分别加热至65℃后混合，然后快速喷涂固化形成涂层。

This paper focused enterprises make certain to choose which type of technological transformation by analyze industry cluster degree, which technological life cycle technology in, and market saturation degree, and then, with cooperating between the imputed one and outputted one, succeed on transfer the obvious and latent information, made some factors like Human resources, organization and technological base recombine to impulse technological level and learning capacity expand forward, and then this will make enterprises technology ability develops by this way: the first step is striped-down only with throughput, and the second is technology absorptive capacity, and the highest is innovate undependably.

论文经过分析认为，企业通过分析原有技术能力所处阶段、技术势差、产业集中程度、技术所处生命周期和市场饱和程度来确定采用何种方式进行技术转移，通过供需双方的合作，成功转移所需的显性知识和隐性知识，使得企业技术能力中的人力资源、组织、技术基础等因素中某一因素大幅度提高或四因素均衡发展，重组整合，推动企业的技术水平和技术学习能力不断向更高层次发展，从而使得企业技术能力从拥有简单生产能力的仿制阶段过渡到技术同化阶段，并最终发展到自主创新阶段。

He made these things all right, and then my timepiece performed unexceptionably, save that now and then, after working along quietly for nearly eight hours, everything inside would let go all of a sudden and begin to buzz like a bee, and the hands would straightway begin to spin round and round so fast that their individuality was lost completely, and they simply seemed a delicate spider's web over the face of the watch.

他把所有的这些事情做完之后，我的表就走得不能再好了，只是有时候要出点毛病：它安安静静地走了将近八个小时的时候，表内部的所有零件突然一下子松开，并且发出蜜蜂一样嗡嗡的叫声，两根针疯狂地转起来，简直都分不清它们原来的面貌，那样子看起来就仿佛在我的表上结了一张纤细的蜘蛛网似的。

For strong convective weather , it classified all historical sample events into 4 weather patterns (like northwest , trough area ,west wind and southwest current), established 4 characteristic fields of 400hPa height of HLAFS , then according to the principle of the pattern match calculate and compare the real-time HLAFS forecast products-2-using the similar method inside the big and small key areas , establish forecast equation , finally gain conclusion .To duststrom weather, the historical samples were sorted into duststrom and severe duststrom types in 5 regions (they are whole area, west, central, middle-west and middle-east of Inner Mongolia ),moreover set up different sample databases about ECMWF fields (including 500hPa height, 850hPatemperature and sea-level pressure).In order to making duststrom forecast at different time level , we first filtered the real-time data by FAX data ,and then used the method of similar range degree to compare the historical data to the actual data of ECMWF .To precipitation weather ,they were divided into 2 types that suit or unsuit airplane artificial precipitation stimulation in line with their emergence time and district .The appropriate weather physical factors come from T106 were chosen to establish the artificial precipitation stimulation prediction model . In the actual application , we can get prediction result as long as use the real-time forecast data of T213 in the prediction model .

针对强对流天气将历史样本天气分为西北气流型、槽区型、西风气流型和西南气流型等4个类型，并建立其HLAFS资料400hPa四种特征场，按照模板匹配的原理，用相似分析方法在大、小两个关键区中对实时HLAFS预报产品进行计算、比较，再建立预报方程，得出结论；对沙尘暴天气将其按区域分为全区、西部、中部、中西部以及中东部5类，再按强弱分别分为强和一般2类，建立ECMWF的3个场(500hPa高度场、850hPa温度场和海平面气压场)的历史资料库，在用传真资料消空之后，用相似离度方法计算实时ECMWF资料，做出不同时次的预报；对降水天气按出现时间和区域分为适合和不适合飞机增雨作业2类，选择T106资料中恰当的气象物理量因子，用BP神经网络算法建立人工增雨降水预报模型，实际应用中将实时T213相关预报资料代入预报模型即可。

The comparative research results showed that:(1)Thediurnal variation of the net photosynthetic rates in the aquatic leaves was a "double-peak", with an various "midday-decline", the time of the peak value appeared at about 10:00 A.M. and 13:00 P.M., while that in the xeric leaves was showed with invarious peak value and "midday-decline"; The diurnalvariation of transpiration ratesof both aquatic and xeric leaves appeared as a single-peak, the appearance time of peak value of the latter was earlier than the former and gradually decreased in the afternoon; The stomatal conductances appearance time of both of them were close to that of photosynthesisrate ,reached to the maximum in the morning and then gradually decreased: Diurnal variation of intercellular CO2 concentrationshows that: both aquatic and xeric leaves increased at first, and then exhibit a tendency of ascending within narrow range.(2) The net photosynthetic rates, photosyntheticallyactive radiation, stomatal conductances and transpiration ratesof both two kinds of leaves showed positive correlation, The correlation degree among them was prominent, there was significant negative correlation between The net photosynthetic rates and intercellular CO2 concentration.

研究结果表明：(1)水生叶片净光合速率的日变化曲线呈双峰型，出现明显的&午休&现象，峰值出现在10：00左右和13：00左右，但旱生叶片表现出的峰型和&午休&现象不明显；两种叶片的蒸腾速率日变化曲线表现为明显的单峰型，旱生叶峰值出现时间早于水生叶，中午过后逐渐下降；两种叶片气孔导度峰值出现时间和光合速率相近，均表现为上午升高至最高，随后逐渐下降的趋势；两种叶片胞间二氧化碳浓度日变化表现为：整体上先下降，然后有一上升趋势：(2)两种叶片的光合速率与光合有效辐射、气孔导度、蒸腾速率呈正相关，全部达到显著水平；光合速率与胞间CO2浓度显著负相关。

The dynamic changes of the the neuron apoptosis and correlative genes in hippocampus of ACET-groups and twice-ACET-groups suggested that ACET can slower the apoptosis by inhibiting p~(53)and prolong the ascending period of p~(53) in 4 days after the seizure,this effect would weaken after 4 days.ACET may inhibit the descending of Bax,prolong its descending period and then promote its expression,in order to weaken the inhibition of the apoptosis during 4 to 10 days after seizure and then promote the apoptosis during 10 to 14 days after.This effect of ACET can be enhanced after treating again within 7 to 14 days later.

埋线组和二次埋线组大鼠海马神经元凋亡及相关基因表达的动态变化结果提示：埋线治疗在癫痫发作后的早期(4d内)可通过抑制促凋亡基因p~(53)蛋白表达、延缓其达峰值的时程，从而减缓神经元凋亡的发生，此作用在4d后减弱；埋线治疗抑制大鼠海马神经元凋亡的作用在癫痫发作后的中期(4d～10d)减弱，甚至在远期(10d～14d)出现促进凋亡的作用，可能是通过抑制Bax蛋白表达的下降、延长其降至低峰值的时程，促进Bax蛋白表达的回升的结果；在中远期(7d～14d)内予二次埋线治疗对该效应有续效叠加的作用。

The reaction of the grumpiest, most graceless man in British public life was to lambast the press for celebrating Beckham's achievement and then drop him for the next United game, and to keep dropping him over the next three months of a season in which the team ended up winning nothing - in large measure because of the confusion Ferguson had sown among the team by saying he was quitting, and then changing his mind; and possibly owing also to the energy and passion he redirected at his highly successful horse, Rock of Gibraltar.

对于失败，福格森由于宣布辞职然后变卦在球队中引起的困惑以及他把大量精力投入了赛马的行为应负主要责任。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。