查询词典 Alcaligenes

The invention discloses a preparing method of bacillus alcaligenes strain Alcaligenes sp., CCTCC No.M206121 of degraded o-nitrophenol, which comprises the following steps: allocating inorganic salt culture medium with dibasic sodium phosphate, monobasic potassium phosphate, manganese sulfate, copperas, addex-magnesium, calcii chloridum, blue copperas, white copperas and hydrogen dioxide solution; adjusting pH value; sterilizing under high temperature; enriching bacterial; sampling from sludge; allocating suspending liquid with soil sample and inorganic salt; seeding into ortho-nitrophenol fresh organic salt culture medium with finite volume seeding quantity; keeping the temperature; culturing on shaker; getting enriched culture; separating and purifying enriched culture in LB solid culture medium.

本发明公开了一种降解邻硝基酚的产碱杆菌菌株及制备方法，产碱杆菌为Alcaligenes sp。，CCTCC No.M206121。其制备步骤是：首先是配制无机盐培养基，成分为磷酸氢二钠、磷酸二氢钾、硫酸锰、硫酸亚铁、硫酸镁、氯化钙、硫酸铜、硫酸锌和双蒸水，调pH值高压灭菌；其次是菌种的富集，从污泥中取样，将土样与无机盐培养基配制成悬浮液，将悬浮液以一定体积接种量接种到含邻硝基酚的新鲜无机盐培养基中，恒温，摇床培养，获富集培养物；第三是将富集培养物在LB固体培养基中分离和纯化。

The resting cell of Alcaligenes eutrophus 112R 4 can catalyze the hydrolysis of hydantoin, dihydropyrimidine and succinimide effectively, but not function to 5-monosubstituted hydantoins or 5,5'-disubstituted hydantoins.

该菌能水解海因、二氢尿嘧啶和琥珀酰亚胺，且对琥珀酰亚胺活力最高，但不水解 5 单取代海因和5 ，5' 双取代海因，因而被确定为含有酰亚胺酶。

The invention use an alcaligenes faecalis as a fermentation strain, using sucrose or glucose commonly used in food industry as carbon source for microbe cultivation, using conventional chemical fertilizer in the agricultural production as nitrogen source and inorganic salt, the medium of microbial growth is prepared by auxiliary yeast powder, aerobic fermentation is processed in the conditions of 30 DEG C, 1.0vvm, 500r/min ventilatiing and stirring, adding specially supplementary food fluid in the fermentation course achieves the following aims:(1) controlling the penetrating pressure of the broth in the hypotonicity condition;(2) controlling the pH of the broth between 4.0 and 7.0;(3) providing abundant nutritious matter such as carbon source and nitrogen source for microbial growth, obtaining Weilan gum broth after 72 hours, obtainin Weilan gum product by drying in 95-115 DEG C and atmospheric pressure.

本发明以一株粪产碱杆菌为发酵菌种，使用食品工业中常用的蔗糖或葡萄糖作为微生物培养的碳源，以农业生产中常用的化肥作为氮源和无机盐，辅以酵母粉配制微生物生长的培养基，在30℃以1.0vvm、500r/min通风搅拌条件下好氧发酵，发酵过程中流加特殊配制的补料液以达到以下目的：(1)控制发酵液的渗透压处于低渗条件；(2)控制发酵液的pH处于4.0～7.0之间；(3)为微生物生长提供丰富的碳源和氮源等营养物质，经72小时得到威兰胶发酵液，在95℃～115℃下常压干燥得到威兰胶产品。

In order to increase the density of cell and give more guides to optimization of batch fermentation, a Study on the characteristics of Alcaligenes faecalis should be carried out.

为了提高菌体阶段菌体的生长密度、对分批发酵有一些优化指导作用，对菌体本身特性进行了研究。

The paper introduced the method and steps of screening alcaligenes from active sluge.Analcaligenes strain which can be used for praducing PHB has heen got.

介绍了从活性污泥中筛选产碱杆菌的方法和步骤，并得到一株产PHB的菌株。

The maximum PHB yield is 5.6g/L by use of the alcaligenes screened.

用筛选的菌株时，PHB的产量高达5.6g／L。

Through re-screening, one strain named as FZ5 was found to have better degradation ability of lubrication oil and identified preliminarily as Alcaligenes sp..

从福州祥坂污水处理厂的活性污泥中分离到12株机油降解菌，经复筛得到1株具有较强降解能力的菌株（命名为FZ5），初步鉴定为产碱菌属Alcaligenes sp。

To study the grown characteristics of Alcaligenes faecalis for Curdlan in Continuous Cultivation, the Kinetics of Alcaligenes faecalis in Continuous Cultivation with carbon source used as a limited substrate were investigated.

以碳源作为限制性底物对粪产碱杆菌进行了连续培养，对其连续培养特性和动力学性质进行了研究。

Through screening lipases, lipase Novozym 435 and lipase Alcaligenes sp were found to show higher activity and better enantioselectivity for the resolution of 2-(2-chloro- 1-hydroxyethyl)thiophene. The optimal reaction conditions were finally achieved by further investigating the effects of solvents, temperature, rotation speed and enzyme concentrations.

通过对不同来源酶的筛选，找到了Novozym 435和Alcaligenes sp两种选择性较好的酶，它们均对该反应具有较高的选择性和较快的反应速度，在此基础上进一步通过对溶剂、温度、摇床转速以及酶用量的筛选，确定了能够有效拆分2-氯-1-(2-噻吩)-乙醇的较佳反应条件。

The results is two strains of bacteria Pseudomonas alcaligenes (No. 1203) and Cellulomonas (No. 1120) that could endure 60 times of the V water quality standard were selected, that is, 2 strains could grow in 6mg Pb /L and 60mg Cu /L media. Another 2 strains could endure 70 times of the standard were attained, Rhodococcus (No. 1314) and Acinetobacter (No. 1410), which could grow in 7mg Pb /L and 70mg Cu /L media.

共筛选出耐国家水质V类污水重金属Pb和Cu 60倍（Pb 6mg/L，Cu 60mg/L）的菌株共2株，1203号菌（产碱假单胞菌Pseudomonas alcaligenes）和1120号菌（纤维单胞菌Cellulomonas）；耐V类重金属Pb和Cu 70倍（Pb 7mg/L，Cu 70mg/L）的菌株2株，1314号菌（红球菌属Rhodococcus），1410号菌（不动杆菌Acinetobacter）。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。