查询词典 African

Presently, in many African countries, the spread of AIDS among the adult population is more than 25 percent, leaving behind millions of orphans. Just think, a few decades ago a person killed a green monkey in order to eat its meat and now, there are 25 million people dead!

目前，在很多非洲国家，爱滋病的传播，在成年人中超过百分之二十五，留下数百万孤儿，试想想，几十年前某人为了啖食其肉而杀了一头绿猴子，现在已有二千五百万人死亡！

The bPRL mammary gland expression vectors were transfected into COS-7 (African green monkey kidney cell) and HC-11 (epithelial cell of mouse mammary gland) cells mediated with lipofectin, respectively. The transfected cells were cultured and harvested. RT-PCR analysis showed that bPRL mRNA was present in both COS-7 and HC-11 cells.

将构建的乳腺特异的表达载体，分别转染COS-7细胞株和HC-11细胞株，收集转染培养后的COS-7和HC-11细胞，通过RT-PCR实验，证实转染的细胞中在mRNA水平均获得了表达。

The results of transient transfection showed that the recombinant plasmid could be lowly expressed in the murine macrophage cell line RAW264.7 and murine melanoma cell line B16, both of which can endogenously express Tim-3, but not in the African green monkey kidney cell line COS-7 in which no endogenous Tim-3 could be detected.

结果 pGL3-Tim-3P2经PCR、双酶切及DNA测序分析鉴定正确无误；pGL3-Tim-3P2转染RAW264.7和B16细胞（两种细胞均可表达内源性Tim-3）24 h和48h，双荧光素酶活性检测显示其启动子相对活性约为pGL3-Basic空载体的2倍；而pGL3-Tim-3P2转染不表达内源性Tim-3的COS-7细胞后检测不到荧光素酶活性。

The African green monkey kidney cell line, Vero, was used as the host for plasmid DNA and MNNG (N-methyl-N′-nitro-N-nitrosoguanidine) was chosen as the DNA-damaging agent.

实验采用非洲绿猴肾细胞株Vero和MNNG(N-methyl-N′-nitro-N-nitrosoguanidine)分别作为质粒DNA的宿主和损伤剂。

AIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS 7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEM T easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N 末端带上含24 bp的flag标签，克隆到pGEM T easy载体并测序，再亚克隆至真核表达载体pcDNA3 1，酶切鉴定正确后采用脂质体法瞬时转染COS 7细胞，Western blot检测flag cbl在细胞中的表达。

A SV40 based shuttle vector pSP189 and African green monkey kidney cell line was appied , which constitute a shuttle vector /mammalian cell system to detect mutagenesis in vitro induced by animal drugs olaqiundox and quinocetone.

本研究将基于SV40病毒的短暂复制型穿梭质粒pSP189与非洲绿猴肾Vero细胞组成穿梭质粒／哺乳动物细胞诱变检测系统，并将该系统应用于兽药喹乙醇和喹烯酮的诱变性检测。

METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cAIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEMT easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N末端带上含24 bp的flag标签，克隆到pGEMT easy载体并测序，再亚克隆至真核表达载体pcDNA31，酶切鉴定正确后采用脂质体法瞬时转染COS7细胞，Western blot检测flagcbl在细胞中的表达。

Still, the term is not usually applied to rock music rooted in the blues -based or other African American music (except as mediated through folk revivalists ), nor to rock music with Cajun roots, nor to music (especially after about 1980) with non-European folk roots, which is more typically classified as world music .

但是，任期不是通常采用的摇滚乐，根植于蓝调型或其他非洲裔美国音乐(除中介民俗revivalists)，也不是摇滚乐与美国路易斯安纳州的根源，也对音乐(尤其是约1980年)与其他非欧洲民族的根，其中较为典型列为世界音乐。

South African Tzu Chi volunteers deliver food and daily supplies to the visually impaired community in Soweto.

南非慈济志工为位在索威托的盲人院带来食物以及物资援助。

Even I, as an African who grew up in Soweto, was left with a lump in my throat, after seeing the tiny curious faces of the learners facing their future with such an incredible sense of hope and determination.

即使是我这样一个从小在索韦托长大的非洲人，在看到这样的情景后都不禁感动了。一群认真好奇的孩子竟然对他们的未来报有这么大的希望和决心。

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。