查询词典 AFP

No clear correlation betweenthe expression level and the level of the structural gene demethylation in embryonicand adult hepatocytes has been observed.

在胚胎和成人肝细胞中，AFP的表达水平和该结构基因的去甲基化水平没有显示出明显的相关性。

Japanese music producer Tetsuya Komuro received a 3-year jail term, which was suspended for 5 years, from the Osaka District Court Monday for cheating an investor out of 500m yen in a 2006 copyright scam, AFP reported.

据法新社报道，大阪地方法院11日对日本音乐人小室哲哉涉嫌诈骗一案做出判决，小室哲哉获刑3年，缓期5年执行。

O fabricate such a hybrid membrane, 3,4,9,10-perylenetetracarboxylic acid-bound thionine moleculeswere initially doped into titania colloids(TiO_2),and then gold nanoparticles and anti-AFP were immobilized onto the composite film in turn.

iO_2成膜能力好，在金电极表面形成大的比表面，有利于增加甲胎蛋白抗体在电极表面的固载量。

Based on the theoretical simulation result to the system of fish type Ⅱ antifreeze protein and ice in vacuum,the trinomial system of fish type Ⅱ AFP-ice-solvent is constructed.

对鱼类Ⅱ型抗冻蛋白-冰晶组成的复合体系在真空条件下进行理论模拟计算的基础上，构建出鱼类Ⅱ型抗冻蛋白-冰-溶剂的三元复合体系。

The unidentified woman, believed to be in her seventies, made the gift after pastors appealed for donations for international aid projects, Krister Klint, a verger at the Caroli Church in the southwestern Swedish town of Boraas, told AFP.

瑞典西南部包瑞斯镇上的卡洛里教会主事牧师克里斯特。科林特向法新社表示，在牧师们吁请大家捐款给国际救援计画后，这位身份不详、年约七十几岁的妇人便捐出这笔善款。

Political reporter Garrett Graff from The Washingtonian told AFP the magazine had been swamped by readers who had seen the May edition showing Obama wearing nothing but swimming trunks and sunglasses, next to the line:"Our new neighbor is hot."

该杂志政治记者盖瑞特·格拉夫说，读者一看到封面之后，各种评论蜂拥而至。照片中，奥巴马仅穿一条游泳裤，带着墨镜，上身赤裸，旁边写着："我们的新邻居很火辣。"

Our friends in France can now read Master Cheng Yen's wisdom in their own language. Retired AFP journalist, James Whiston, has translated the Jingsi aphorisms, also known as Still Thoughts, into French. The volunteer says it is his way of fulfilling his lifetime dream of becoming a missionary.

现在居住在法国的朋友也能以法语阅读证严上人的静思语，一位已经退休的法新社记者詹姆士，已经将静思语翻译成法文版，志工说，这是他传道，修行的终身梦想。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Methods By using Raten Nephelometry method,the serum α 1 Antitrypsin were detected from some nomal persons and some patients with any kinds of liver disease,especially those PHC patients with AFP negativity.

-抗胰蛋白酶(α1 -Antitrypsin，α1 -AT)，是具有蛋白酶抑制作用的一种急性时相反应蛋白，分子量为 5 。4万，在醋酸纤维薄膜或琼脂电泳中位α1 -球蛋白区，主要由肝脏合成。

After Bel-7402 was treated with melittin, detection methods were applied as followed, 1 measure the change of proliferation by MTT ; 2 light microscope and transmission electron microscope were used to observe the morphous and biological behaviour and ConA agglutination test and clonality test were applied to measure the adhesion capability and growth potency of Bel-7402; 3 ELISA , colorimetry , bromocresol green mehod were used to determine AFP level, the activity of y - GT and ALP, ALb level respectively; 4 the change of c-myc gene expression was detect by immunohistochemical method.

观察蜂毒素作用于人肝癌Bel-7402细胞株后(1)MTT方法测定对肿瘤细胞增殖的影响(2)光学显微镜和透射电镜下观察对形态及生物学行为的影响，ConA凝集试验及克隆形成能力反映处理后细胞粘附能力及生长潜能(3)ELISA法测定甲胎蛋白含量、重氮反应比色法测定γ-谷氨酰转肽酶活力、比色法测定碱性磷酸酶活力、溴甲酚绿法测定白蛋白含量，检测对肝癌细胞代谢与分泌的影响(4)免疫组织化学法检测处理前后c-myc基因表达的差别。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。