查询词典 ADM

By immunohistochemistry and semiquantitative RT-PCR analysis, we were the first to demonstrate that AdM mRNA and immunoreactive AdM exists in the rat brain distributed in cerebral cortex, paraventricular tissues, hypothalamus, mesencephalon, medulla oblongata and cerebellum, and AdM mRNA was also found in the human brain.

应用免疫组织化学和半定量RT-PCR方法，首次发现在大鼠的脑内，包括大脑皮层、室旁组织、下丘脑、中脑、延髓和小脑均有AdM mRNA及AdM的免疫活性物质存在；在人脑内也含有AdM mRNA。

As metal ions can interact with both of ADM and DNA, they undoubtedly affect the interaction between ADM and DNA. Addition of a large excess of Cu or Fe into a solution of well-formed ADM-DNA complex makes the metal ions simultaneously bind to ADM and DNA to form a ternary complex. Otherwise,〓 or 〓 complexes can bind directly to DNA to form a ternary complex.

金属离子-ADM-DNA三元体系的光谱研究表明，当ADM与DNA结合后，大量Cu或Fe的加入可以使金属离子进入到ADM-DNA复合物中，并同时与ADM和DNA键合，形成三元复合物；反之，若ADM先与Cu或Fe配位，生成的金属配合物则能够直接与DNA结合形成三元复合物。

Methods:(1) ADM was produced from swine skins treated with trypsin followed by Triton X-100.(2) type I collagen of mouse tail was extracted by 0.5mol/L acetoacetic acid.(3) Two kinds of ADM were got by being soaked with 0.02% glutaraldehyde for 5~10 min or being soaked with mouse type I collagen for 24h, then preserved at 4℃.

(1)应用胰蛋白酶消化-去污剂法制备ADM；(2)用0.5mol/L乙酸溶液提取制备I型鼠尾胶原；(3)用0.02%戊二醛溶液浸泡ADM5-10min，制成交联型ADM；另一部分ADM用质量分数0.25%的I型鼠尾胶原浸泡24h，制成胶原包埋型ADM，冰冻保存；(4)用酶消化法培养原代成纤维细胞，取第三代增殖期细胞，将其调整至浓度为2×105/ml的成纤维细胞悬液。

In structure-function analysis of AdM, it showed that the carboxy terminal 22～52 amino acide fragment is important and necessary for the expression of the cardiovascular activity of AdM.

应用人工合成的AdM的不同片段给麻醉大鼠侧脑室注射，发现AdM的22～52氨基酸片段是维持其活性所必需的。

Heat treatment can apparently confronte multidrug-resistant of 7721/Adm and increase the sensitivity of 7721/Adm to ADM.,and the resulet indicate that increasing the sensitivity can be related to intracellular concentration of ADM.

结论加热可以显著对抗7721/Adm的耐药性，提高其对阿霉素的敏感性，这与加热提高了细胞内药物浓度有关。

Six weeks after drug intervention,we found,in ADM+Res group,the mean tumor volume of MCF-7/ADM cells implanted tumor was 1.203±0.338 cm~3 with statistical significance to compare with other groups(P＜0.05),decreased about 50.58%compared with the mean tumor volume of ADM alone group1.979±0.307 cm~3the result of the mean tumor weigh was alike to the mean tumor volume; compared with the control,Res could relieve weigh loss(P＜0.05,and the toxicity of treatment was not obvious;Microscopic examination showed that there was massive area of cytoclasis and apoptosis in the tumor tissue of the combination group comparing with other groups.

实验结果提示用药6W后，ADM+Res组移植瘤体积(1.203±0.338)cm~3明显小于其余各组，抑瘤率为50.58%，与单用ADM组(1.979±0.307)cm~3相比有差异P＜0.05肿瘤重量的结果与瘤体结果类似；未观察到用药后的毒副作用；经不同处理后，单独注射Res组小鼠体重较生理盐水组及ADM组有所增加(P＜0.05，而ADM+Res组治疗前后体重无差异P＞0.05HE结果显示与其他组相比，ADM+Res组肿瘤组织中出现大片坏死区域。

With 〓H-TdR incorporation and cell count, we found that AdM could inhibit DNA synthesis and the proliferation of VSMCs from SHR. With semiquantitative RT-PCR analysis, we found that AdM mRNA level was lowerer in kidney and VSMCs from SHR than those from WKY.

应用〓H-TdR掺入和细胞计数发现，AdM可以抑制培养的自发性高血压大鼠VSMCs的DNA合成和细胞增殖；应用半定量RT-PCR分析发现，在自发性高血压大鼠的肾和VSMCs中，AdM mRNA水平较低。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group）.The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group）.The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组（K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组），采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量，流式细胞术检测细胞内阿霉素的浓度，基因、酶学、蛋白水平检测实验分3组（K562组、K562/A02组和K562/A02+TTD组），采用RT-PCR法检测mdr1 mRNA的表达，免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平，Western-blotting法检测P-糖蛋白和bcl-2表达。

After ADM receptor antagonist was given, effects of ADM were antagonized.

加入外源性ADM受体抑制剂后，可以拮抗ADM的作用。

Except RDW,MPV and LYM pa-rameters,ADM could induce almost every parameter,espe-cially the RBC,WBC and PLT,to decrease significantly,which was more clear with the increasing of ADM dosage.

ADM可引起外周血除红细胞分布活力、平均血小板体积和淋巴细胞百分比外几乎所有的指标降低，红细胞、白细胞、血小板3种血液成分降低显著（P.05），并随ADM剂量的增加而愈发明显。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失