查询词典 A.I.A.

However, the fact is when I told my name to a new friend, they immediately asked me "Oh, is it W-E-I?" I just feel like I was self-righteous... Anyway, it was good to hear they know about chinese phoneticization.

不过万万没想到的是无论去年也好还是今年，但凡我念了我的名字给他们听之后他们都懂得要拼成W-E-I，实在让我感觉自己是在自作多情……不过得知他们都懂得中文的拼音还是挺欣慰的。

As for Seba… Well, I once tried to explain to my husband that a measureof a player's quality is whether, if I normally do not consider themattractive, I think they're sexy after watching them play.

说到塞巴嘛……怎么说呢，我曾经尝试过跟我老公解释，当描述一个球员的质量的时候，如果我通常不认为他们有吸引力的话，我会在看他们踢球之后觉得他们很性感。

Emanuel is the person who I called as uncle normally......Sonia was here for tea tonight. While we were having tea, she suddenly said "Shelvy, I think your english has been improved a lot."

今天Sonia来我们这吃饭，吃着吃着她突然来了一句：&Shelvy， I think your english has been improved a lot。&

A multimedia file I/O service provides buffered and unbuffered file I/O.It also supports the standard IBM /Microsoft Resource Interchange File Formatfiles.These service are extensible with custom I/O procedures that can be shared among applications.

提供有缓冲和无缓冲的文件I/O，它还支持标准的IBM/Microsoft资源交换文件格式文件，这些服务还可由用户I/O过程扩展并在应用程序间共享。

" Q" N '2 V+ i' R# Y; O This page belongs to the public resource, which is a window to know Binzhou and show the beautiful image of Binzhou to the friends from the world, so most of all is the unforbidden images searched from the internet. When you find the page has used your original works without your signature, please answer the page or tell me by short message and I will sign for you. If you don't agree with the reference, I will change it in time and apology to you.

U$ V。 b6 S {1 t8 a滨州论坛本帖属公共资源，是了解滨州的一个窗口，是为了向五湖四海的朋友集中展示滨州美景，所以一部分图片是摄影师赞助，一部分为从网上搜集的未表示不许引用的作品，当您发现帖子里引用了您的原创作品而未署名，请您跟帖或短信告诉我，我将为您署名，如果您不同意引用也请跟帖告知，我将及时更换并向您道歉。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

According to the a cad emic records I have achieved so far, I am confident that I will get my Bachelor of Business Administration this coming July.I: How do you think the education you have received will contribute to your work in this institution?

根据至今我所取得的学习成绩，我敢肯定今年7月份我一定能获得商业管理学士学位。I：你认为你接受的教育将如何有助于本机构的工作？

I'm Miss American Dream since I was 17 Don't matter if I step on the scene Or sneak away to the Phillipines They still got pictures of my derriere in the magazine You want a piece of me?

You want a piece of me…我从17岁起就是个美国梦女郎我正跨步前进着，但这不并重要或者就像悄悄的逃到菲律宾去一样他们把我的美臀放到了杂志里你想知道我的点点滴滴吗？

I still aux would rather when SA or SC, want to let oneself enter that range among bovine A and bovine C all the time, my this person, now and then oestrus also is flashy, resemble man passion momently same, my meeting looks for a person wildly to chat, talk about relation of male and female, I want to look for a girlfriend, exclaim so all the time, but the society never becomes this topic a fart in light of.

我还是宁愿当SA或者SC，一直想让自己进入牛A与牛C中间的那个行列，我这人，偶然发情也是一瞬间，就像男人激情一刻一样，我会疯狂的找人聊天，聊男女关系，我想找个女朋友，一直这样呼喊，可是社会从来没有把这个话题当个屁来看。

I also would like to focus teacher said: If I was to fly the Youth Pre-employment Training birdies, then you are dark-winged I wing the wind, if I was just germination of a new leaf, then you are deep in the earth - Gen.

我也想集中，老师说：如果我是飞展翅小鸟，那你就是黑暗翼i翼风，如果我是刚刚萌发的新的一页，那你就是在深部地球-将军

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。