查询词典 A.G.

Then the combinant plasmid was conducted into ACC-2 cell by electroporation. ACC-2 cells stably expressing CD was obtained by 10-day positive selection with 400 μg/mL G418. Total RNA was extracted and the expression of the CD gene in transfected ACC-2 cells was identified by RT-PCR. RESULTS: PCR yielded a fragment of l280bp and CD was verified by sequence analysis.

测序正确后，将其亚克隆到质粒表达载体pIRES中，构建以内部核糖体进入位点相连的CD基因的质粒表达载体pIRES-CD，采用电穿孔法，以质粒表达载体转染ACC-2细胞，用400μg/mL的G418筛选10d，获得稳定表达CD基因的ACC-2细胞系，提取该细胞的总RNA，用RT-PCR检测CD基因的表达。

The molar ratio of sulfate ions to carboxylate ions is determined by conductometric titration method and the molecular weight is tested by high performance gel permeation chromatography. The precision of these two methods is also studied. To improve the detection sensitivity, guanidine hydrochloride is used as the best derivation reagent of 911. A systematic research is done on the optimum derivatization condition of 911 and Guanidine hydrochloride. The M /G ratio of 911 has direct relation to its biological activity.

论文首先对911的基本理化性质进行了系统的分析，以电导滴定法和高效凝胶渗透色谱法分别测定了分子中硫酸根羧酸根的比值和911的分子量，并探讨了两种分析方法的精密度。911分子没有紫外吸收，为提高检测灵敏度，本文采用荧光标记法，通过对三种荧光试剂与几种寡糖和多糖衍生效果的综合比较，最终确定了盐酸胍为最佳衍生试剂，并以911为代表详细探讨了衍生化反应的最优条件。911糖链结构与褐藻胶主链结构类似，以甘露糖醛酸和古罗糖醛酸的嵌段形式存在，分子中M/G比值与911的物理化学性质和生物活性有直接关系。

To identify whether our previous founding were exist in the series of our newly established cell cultures with the same genetic background and different metastatic potentials, and to make clear what are congenerous and various traits in the chromosomal or DMA sequence levels between these cells, in this study, a combination of conventional G-banding, comparative genomic hybridization, multiplex fluorescence in situ hybridization and arm or locus-specific fluorescence in situ hybridization were used to comprehensively characterize molecular cytogenetics aberrations of the above cell cultures.

为查明我所新建立的这些具有同一遗传背景而又在肝癌的转移潜能上存在明显差异的细胞是否也具有8p的缺失，并弄清它们在其它染色体及其区段的DNA水平上有着怎样的相似和不同，这些相似与不同又有着怎样的意义，本课题联合应用G显带技术、比较基因组杂交技术、多重荧光原位杂交技术和位点或臂特异荧光原位杂交技术，对上述细胞进行细胞及分子遗传学研究，以期了解其分子细胞遗传学异常的特征、找出转移相关的遗传学标记，为探讨肝癌转移机制提供材料。

His meeting, which may almost be designated as his union, with conventionary G----, left behind it in his mind a sort of astonishment, which rendered him still more gentle.

他和那国民公会G。代表的邂逅棗几乎可以说是他们的结合，只不过给他留下了一种使他变得更加温良的惊叹的回忆。

The decision making method of uncertain multi-attribute is applied to select bamboo species for constructing a project on 500 thousand mu(equivalent to 33350 hectare) pulpwood base of bamboo pulping paper in Jinghong.In detail,combined G with Zi based on OWA,it concludes that Z4=Lingnania distegia,Z2=Dendrocalamus hamiltonii,Gx1=Dendrocalamus giganteus,Gx2=Dendrocalamus peculiaris are finally selected as bamboo species for base target management;Z16=Dendrocalamus barbatus as prepared bamboo...

应用不确定多属性决策方法为拟建的《景洪竹浆纸项目50万亩竹子原料林基地》项目的热带大型丛生竹选择竹种，将多准则模糊决策方法的满意度G值和基于OWA算子的多属性决策方法的综合属性值Zi结合起来考察，最终选定：Z4=料慈竹，Z2=版纳甜龙竹，Gx1=龙竹，Gx2=金平龙竹4个竹种作为基地的目的经营竹种；Z16=小叶龙竹作为将来发展的储备竹种。

objective to analyze the drug resistance of staphylococcus aureusand provide suggestion for clinical rational use of antibiotics.methods secretion of those neonates suffered primary skin soft tissue infection and admitted during june,2005 to december,2007 was cultured and 113 strains of staphylococcus aureus were detected.we used k-b diffusion methods to determine their susceptibility to the common used antibiotics.results resistance rates of staphylococcus aureus to ampicillin/sulbactam,penicillin-g and cefazolin were 14.2%,40.7%and 6.2% respectively; there was no vancomycin-resistant strain.conclusion the resistance of staphylococcus aureus is not a serious problem in neonatal dermatic soft tissue infection.local treatment is the major approach in clinic,while general anti-infection consisting mainly of penicillin and first generation cephalosporin is chosen to avoid resistance mutation of staphylococcus aureus resulted from abuse of antibiotics.

多重聚合酶链反应快速鉴别葡萄球菌及。。。乳腺脓肿分离到葡萄球菌的鉴定和药敏。。。目的探讨金黄色葡萄球菌的耐药性，指导临床合理用药。方法对我院2005年6月至2007年12月新生儿原发性皮肤软组织感染患儿的分泌物进行培养，共检出金黄色葡萄球菌113株，并采用kb纸片扩散法对其进行常用抗生素的药敏试验。结果金黄色葡萄球菌对氨苄西林/舒巴坦、青霉素g及头孢唑啉的耐药率分别为14.2%、40.7%和6.2%，未发现耐万古霉素金黄色葡萄球菌。结论在新生儿皮肤软组织感染中金黄色葡萄球菌的耐药问题并不十分严重，临床处理应以局部为主，全身抗感染治疗宜以青霉素及i代头孢类抗生素为主，避免过度使用抗生素加速金葡菌的耐药突变。

The results showed that when 0.3 g of divinyl benzene was used as crosslinking agent, the crosslinking degree increased significantly from 28.26% to 90.95% with the increase of MAA amount from 4.0 mol% to 10.0 mol%, and this increase became slowly as MAA amount was more than 10.0 mol%; the particle volume expansion was increased gradually as MAA amount increased, and reached a maximum of 42.1% when 12.0 mol% of MAA was used, and perfect porous morphology inside particles was observed even when the crosslinking degree reached 95.4% at 14.0 mol% of MAA.

结果表明：在交联剂二乙烯基苯用量一定(0.3 g)的条件下，随着MAA用量从4.0 mol%增加到 10.0 mol%，聚合物交联程度从28.26%迅速增大至90.95%，当其用量超过10.0 mol%后 P x增大趋势变缓；随着MAA用量的增加，处理后胶粒体积膨胀百分率逐渐增大，在MAA用量为12.0 mol%时Δ V 达到42.1%的最大值，当MAA用量增大到14.0 mol%时，尽管 P x高达95.44%，胶粒仍具有完好的孔状结构。

Under the conditions of q=2, G is a finite group of order mn ,where both m and n are odd prime numbers, the splitting is μ-1 , the necessary condition and sufficieat condition for the existence of such duadic codes are presented.

考虑q＝2，G为mn阶非阿贝尔群且劈分为μ－1的duadic码存在的充分条件和必要条件。

Results demonstrate that the Cu(superscript 2+) can be concentrated effectively under the following condition: exchange column internal diameter 0.8 cm and 15 cm high, the dosage of spiral algae 2 g and pH value 6, the flow rate of the dry food leach solution 10 mL/min, elute with 0.6 mol/L HCl at a flow-rate of 6 mL/min.

结果表明，在交换柱内径为0.8 cm、柱高为15 cm、螺旋藻用量为2 g、pH值6的条件下，干制品浸出液流速为10 mL/min时，用0.6 mol/L HCl以6 mL/min的速度洗脱，可对Cu(上标 2+)进行高效富集。

A nano-Si composite anode material was found to exhibit an extremely high reversible capacity over 1700mAh/g Si, good cyclic performance and endurability under larger current density.

研究发现纳米硅复合负极材料在室温二次锂电池中表现出较好的循环性，可逆容量达到1700mAh／g，而且具有良好的动力学行为。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐