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The in vitro effects of Gln49-PLA_2 were examined using the chick biventer cervicis nerve muscle preparation. Gln49-PLA_2 caused a dosedependent blockade of neuromuscular transmission. The time required to cause 50% blockade was about 150 min at the highest concentration 100μg/ml. This inhibition appeared to be presynaptic in origin as evidenced by the lack of effect of toxin on responses to exogenous acetylcholine in the non-stimulated BCp.

Gln49-PLA_2能够剂量依赖性地抑制颈二腹肌神经信号正常传导并影响肌肉收缩，在100μg/ml剂量下，肌肉收缩幅度在150分钟后达到50%的抑制率；同时，随着Gln49-PLA_2浓度的降低，肌肉收缩幅度的抑制效率也逐渐降低；实验证明Gln49-PLA_2对突触后膜乙酰胆碱受体敏感性无影响，对神经肌肉信号传导收缩的抑制作用位点在突触前膜。

The study of treating dye wastewater with iron scraps and fly ash for electrochemistry principle;2. A study of the reaction on the treatment for blick G dye wastewater using iron scrapshas been carried on by the authors.

研究了废铁屑处理黑G染料水样的反应过程及处理效果：反应介质的pH值和反应停留时间为影响反应效果的两个主要因素，pH值越小，反应时间越长，处理效果越好；停留时间在4～10min，反应过程已基本完成；色度去除率为92％～98％，CODcr的去除率为40％～75％。

For the DPPH free radical scavenging, their EC50 values are 19.05 mg/ml and 15.61 mg/ml respectively, but their anti-oxidant capacities are 40 to 60 % higher than fresh ones. Hot water extracts of Boehmeria nivea root experienced sun dry treatment have a better ability to scavenging ABTS free radical (EC50=34.16 μg/ml)when compared to that of fresh root, which only have one tenth anti-oxidant ability left.

经冷冻乾燥和日晒乾燥的苎麻叶热水萃取物其抗氧化成分较新鲜者低约55至67 %，而对DPPH自由基清除能力其EC50分别为19.05 mg/ml和15.61 mg/ml，相对其抗氧化能力却较新鲜者为高约40至60%；经日晒乾燥苎麻根热水萃取物其清除ABTS自由基能力(EC50=34.16 μg/ml)明显较新鲜者为高，显示其清除自由基能力只剩1/10。

Results: Bornyl alcohol showed a good linear relationship at the range of 0.25～0.75 g/L,r=0.999 9. The average recovery rate was 99.33% and RSD was 1.4%.

结果： 龙脑在0.25～0.75 g/L线性关系良好，r＝0.999 9，加样回收率为99.33 %， RSD为1.4 %。

Results: BIO-1211 aerosol at 1, 3 and 10 mg/ml significantly inhibited the changes in lung resistance and lung dynamic compliance after antigen challenge in the sensitized rats in a dose-dependent manner, BIO-1211 at 25, 50, 100 and 200μg/ml inhibited the fibronectin-induced neutrophil adhesion by 23.5%, 24.6%, 61.4% and 58.1%, respectively, and serum-induced adhesion by 29.9%, 35.9%, 35.3% and 15.4%, respectively. Inhalation of 10mg/ml BIO-1211 did not show any protection against histamine and actetylcholin-induced bronchoconstriction.

结果：给大鼠气雾吸入BIO-1211 (1、3、10mg/ml)呈剂量依赖抑制致敏大鼠抗原攻击引起RL的升高和Cdyn降低；BIO-1211(25、50、100、200μg/ml)明显抑制纤维黏连蛋白和血清诱导的中性粒细胞黏附反应，其抑制率分别为23.5%、24.6%、61.4%、58.1%和29.9%、35.9%、35.3%、15.4%；但BIO-1211 (10 mg/ml)给豚鼠气雾吸入不能抑制乙酰胆碱和组胺诱导的药物性哮喘。

A novel type of composite electrode based on multiwalled carbon nanotubes coated with sheet-like cobalt hydroxide particles was used in supercapacitors. Cobalt hydroxide cathodically deposited from Co(NO3)2 solution with carbon nanotubes as matrix exhibited large pseudo-capacitance of 322 F/g in 1 mol/L KOH.

采用电化学阴极沉积还原Co(NO3)2的方法制备了具有准电容特性的氧化钴电极材料，其比容量达到280 F／g，采用CNTs作为电极基体，在其表面均匀的沉积了纳米钴化镍颗粒并由此制备了氧化钴碳纳米管复合电极材料。

Penicillin V acylase from the crude culture filtrate of Fusarium oxysporum FP 941 was purified with a single band on non-denaturing electrophoresis by means of (NH4)2SO4 fractional precipitation, adsorption on, and elution from, celite, ion-exchange column chromatography with DEAE-cellulose, and Sephadex G-200 gel filtration chromatography.

通过硫酸铵沉淀、硅藻土吸附、DEAD-纤维素离子交换层析和Sephadex G-200凝胶过滤，由尖镰孢FP941培养滤液中得到了聚丙烯酰胺凝胶电泳均一的青霉素V酰化酶。

(1) In the sorption kinetics experiment, Cu2+ sorptions by Ceratophyllum demersum, Myriophyllum triphyllum and Hydrilla verticillata respectively, all reached balance within 35mins, all of the test results of sorption kinetics recommended the pseudo second-order equation and indicated that this kind of sorption was a complicated process of simultaneous actions of many reactions. The test results of thermodynamic sorption fitted with Langmuir model better than Freundlich model, and the maximum sorption quantites were 13.12, 20.12, 7.79mg/g, respectively,In the desorption test, the desorption rates of each plants were all below 1%, indicating strong sorption-holding action of these submersed mcrophytes on Cu2+.

通过上述研究，得到以下结论： 1、狐尾藻、金鱼藻、黑藻在对铜离子的吸附动力学实验中，均在35min内达到吸附平衡，分别用一级动力学方程和伪二级动力学方程拟合，结果均为用伪二级动力学方程拟合效果较好，表明该吸附为多种反应同时作用的复杂过程；在热力学实验中，三种植物的吸附曲线都能较好的用Langmuir曲线拟合，其拟合最大吸附量分别为13.12、20.12、7.79 mg/g；在解吸实验中，三种植物的解吸率都较低，说明对铜离子都有较好的吸持能力。

A determination method was established for quercetin and kaempferol by HPLC. The contents of quercetin and kaemprol in Chenopodium ambrosioides L.

采用HPLC建立了槲皮素和山柰素的含量测定方法，土荆芥中槲皮素和山柰素的平均含量分别为0.25mg/g和1.03mg/g。

METHODS: 10 mL bone marrow was extracted from dog posterior superior iliac spine, heparin anticoagulation, diluted using Hanks juice, treated with 1.077 g/mL Ficoll solution 3 mL, and centrifuged at 2 000 r/min for 20 minutes. Karyocytes were absorbed to form white cloudlike layering interface, and then centrifuged twice using DMEM supplemented with fetal bovine serum, incubated at 12×104/cm2 at 37 ℃ in a 5% CO2 incubator.

于犬髂后上棘抽取骨髓液10 mL，肝素抗凝，Hanks液稀释后，加入1.077 g/mL Ficoll液3 mL，2 000 r/min离心20 min，吸取有核细胞形成白色云雾状的分层界面，用含胎牛血清的DMEM离心2遍，按12×104/cm2密度接种，于37 ℃、体积分数为5%的CO2培养箱内培养。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐