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Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

To develop novel live attenuated influenza vaccine, we explored the feasibility to attenuate influenza virus by codon deoptimization of NS1. According to the codon usage bias in influenza A virus, we designed and synthesized a condon-deoptimized NS gene by substituting codons of 110 amino acids in the NS1 gene of A/Puerto Rico/8/34(H1N1) with unpreferred synonymous codons.

根据A型流感病毒密码子使用偏嗜性，选取稀有密码子对A/Puerto Rico/8/34(H1N1)病毒NS1基因内部110个氨基酸区域进行密码子同义突变改造，并全基因合成NS基因，利用反向遗传操作技术拯救出含有密码子去优化NS1基因的重组病毒。

The levels of SP-A of recurrent vulvovaginal candidiasis group were higher than those of VVC group and control group, and the expression of SP-A mRNA in VVC was higher than that in the control group.

用ELISA方法检测各组中SP-A蛋白的含量发现，VVC组的SP-A明显高于对照组(P.05)，RVVC组的SP-A高于对照组及VVC组(P.05)。

A freeman shall not be amerced for a slight offense, except in accordance with the degree of the offense; and for a grave offense he shall be amerced in accordance with the gravity of the offense, yet saving always his "contentment"; and a merchant in the same way, saving his "merchandise"; and a villein shall be amerced in the same way, saving his "wainage" if they have fallen into our mercy: and none of the aforesaid amercements shall be imposed except by the oath of honest men of the neighborhood.

凡由我们进行裁处的按如下执行：自由人犯轻罪，非根据犯罪程度科罪的，不得处以罚款；犯重罪的，根据严重程度科处，但须保留其必要的生活用品；商人犯罪，同样处刑，但得保留其货品；维兰犯罪亦同，但得保留其农具；并且，非经诚实邻人宣誓作证，不得加刑。注： a 、第二次重签时插入&他人而非我们的&。b 、参见第5条注 b 。c 、此处开始发现陪审团的渊源，不过据说在这种情况下，所作出的处罚相当严酷。

The information that lives in business software systems mostly comes down to collections of words about the application domain--maybe with a few special symbols mixed in. Internet communications protocols consist mostly of a few special words used as headers, a little bit of constrained formatting, and message bodies consisting of additional wordish texts.

的资料，生活在商业软件系统主要归结为收藏的话，应用程序域-也许与a几个特殊符号混合个互联网通信协议的组成主要是a一些特殊的话作为标题，a有点限制格式和信息机构组成的额外wordish文本。

Further studies are underway to investigate the exact intracellular mechanisms underlying the effects of the two substances. In Chapter 4, we observed the effects of metabolites of mangrove fungus Xylaria sp. from the South China Sea Coast, Xyloketal A～D, on the activity of acetylcholinesterase in vitro, and the present data indicated that AChE activity was remarkably inhibited by Xyloketal A～D in a dose-dependent manner.

另一方面，在论文第3章离体豚鼠回肠收缩实验中，发现323-A和323-B可剂量依赖性地抑制组胺、乙酰胆碱以及氯化钾所引起的GPI收缩，pD2'依次为5.13、4.97、5.36和5.51、5.56、5.62，并且，通过机制分析发现，323-A和323-B可以显著降低在外界无钙离子存在时由细胞内钙储库释放钙所产生的收缩反应，而对由细胞外钙离子内流所引起的收缩无明显影响。

Settlers from Scandinavia, Germany, Ireland, and Russia, as well as elsewhere in Europe and from the eastern US states increased from a trickle to a flood, especially after the completion of an eastern railway link to the territorial capital of Yankton in 1872, and the discovery of gold in the Black Hills in 1874 during a military expedition led by George A.

定居者从斯堪的纳维亚，德国，爱尔兰，俄罗斯，以及欧洲其他地区和美国东部的国家增加了从滴入到洪水，特别是在完成东部铁路的领土首都扬克顿1872年，和发现了金矿的布莱克山于1874年在一次军事远征由乔治A。

The reacting tite with NDPK-A was 1:800 and theantisera was specific.Conclusions: The purified rhNDPK-A protein exists mainly in the form of hexamer;rhNDPK-A protein can promote the survival of spleen cell and may take part in theadjustment of cell growth by the way of changing cell cycle.

ELISA法检测所制备的抗体的反应效价为：1：800，western blotting试验结果证实，该抗血清具备较好的特异性，能与rhNDPK-A蛋白发生反应，结论：rhNDPK-A蛋白可能主要以六聚体形式存在，直接观察的蛋白结构与文献报道的X衍射结果相似；rhNDPK-A蛋白对正常小鼠的脾细胞的存活有促进作用，推测很可能以信号转导的方式调控细胞周期从而达到对细胞生长的调节作用。

Rodriguez hasn't stopped hitting, either, batting .360 since that breakout game as the Yankees, comfortably in control of the AL East, play carefree baseball. He rediscovered his smooth footwork in the field, and his hands felt faster at the plate. He began to wait long enough on pitches to drive them hard to centerfield and rightfield, the satisfying confirmation for a righthanded hitter, like a wink from a pretty girl, that life is good.

A-Rod的手感还持续发热，自从那场对上老虎队的比赛后，他的打击率高达。360，而洋基的战绩也继续笑傲美联东区， A-Rod重新找回了他在守备时的顺畅脚法，以及打击时的挥棒速度，他也开始等到最后一刻才出棒然后将球狠狠地捞到中右外野，这对右打者来说是个令人欣慰的现象

In the top of the fifth, Posada helped Wang do just that. Rodriguez lined a single into the gap in left-center and reached second when Joey Gathright misplayed it. After Hideki Matsui walked, Posada - batting righthanded against lefthanded reliever John Bale - lifted a long fly to rightfield on which Teahen turned the wrong way. Possibly affected by the wind, the ball wound up hitting the base of the wall for a two-run double that gave the Yankees a 5-3 lead. www.wangfans.com; h2 E# 7 \/ f* i

而在五局上，胖波帮助小民做到这点，A-Rod打出穿越中间方向的安打并且造成Joey Gathright的失误，在松井秀喜被保送后，面对后援的左投John Bale而站在右打击区的胖波，击出右外野方向的高飞球，而Teahen接球时转向错误的方向，也许是受到风的影响，那球打在全垒打墙上形成2分打点的二垒安打让洋基取得5：3的领先。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。