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Homology modeling of 3-D structure of two AChE from L.entomophila were constructed using H.sapiens(1p0i:A) native BuChE structure and Drosophila melanogaster(1d×4:A) native AChE structure as templates,respectively,by SWISS-MODEL.The catalytic triad were found and denoted in the 3-D structure of AChE from L. entomophila referring to T.californica.2.2 Gene cloning ofβ-actin and mRNA expression levels of two AChE genes from L. entomophilaBecause no reference gene has ever been used in Real Time PCR for L.entomophila in GeneBank,a fragment ofβ-actin gene was cloned from L.entomophila(GenBank Accession No.: FJ041117).It consists of 822 bp encoding a protein of 273 amino acids residues.

利用蛋白质结构同源建模工具，分别以人丁酰胆碱酯酶(1p0i：A)和果蝇乙酰胆碱酯酶(1d×4：A)的蛋白晶体结构为模板，对嗜虫书虱2个AChE的三维结构进行同源建模，并在三维结构中发现了AChE的酶解活性位点，证明嗜虫书虱体内也存在2个AChE基因。2.2嗜虫书虱β-actin基因克隆及乙酰胆碱酯酶基因mRNA表达水平研究目前关于嗜虫书虱的分子生物学研究较少，在GenBank中没有可用作内参基因的序列，因此本研究从嗜虫书虱体内克隆获得β—actin基因片段(GenBank登录号：FJ041117)，该片段长度为822 bp，编码273个氨基酸残基，同源性比对分析表明该片段与其它昆虫的β—actin基因具有很高的同源性。

Results:Wound healing rate in group A was higher than that in group B and the wound healing time was 1～3 days earlier in group A.More active proliferation of epidermic cells around wounds showed in group A.The function of fibroblasts in wounds,collagen synthesis and secretion,were more vigorous in group A than those in group B.

结果：A组创面愈合率高于B组，其创面愈合时间较B组提前1～3 d；创周表皮细胞增殖也较B组活跃，创面成纤维细胞较B组呈现出更旺盛的合成与分泌胶原纤维的功能。

Within sampling depth,accumulation rate ranges from 0.02mm/a to 2.58mm/a for these sections,and a significant erosional/depositionalhiatus is identified for two natural sections.

在取样的深度范围内，所测剖面的堆积速率从0.02mm/a到2.58mm/a，并显示一般风成黄土的堆积速率要比水成堆积物的堆积速率要慢。

However, the 3 groups from medium A exhibited the highest microbial diversity and best decolorization results with 99.53% and 97.42% color removal rate of Reactive Red M-3BE and Acid Red. From them, 16 strains of fungi were isolated and primarily identified as Saprolegniaceae, Eurotiaceae, Erysiphaceae and Physodermataceae . Fungi groups from medium B and D exhibited a bit lower color removal rate of various dyes and only 3 and 2 isolates primarily classified as Saccharomycetaceae and Eurotiaceae were obtained from them. Fungi cultures in medium A and B could produce lignin peroxidase, and those in medium D could be detected higher activity of laccase. All the fungal cultures exhibited very weak activity of manganese dependant peroxidase.

来自A培养基的3组菌群显示出最好的脱色效果和最大的菌群丰富度，对50mg/L的活性红M-3BE和酸性红A溶液的脱色率最高达到99.53%和97.42%，从中分离到了16株真菌，初步鉴定分属于水霉科、曲霉科、节壶菌科和白粉菌科；而B和D培养基中所获得的菌群脱色效果稍差，从中仅得到3株和2株真菌，初步鉴定属于酵母和青霉。A、B两种培养基在各种染料存在下更易产生木质素过氧化物酶，产漆酶能力较弱，而D培养基产漆酶活性较高。

METHODS: After stimulated with Con A, T cells were treated with different concentrations of TMB-8 alone and its combination with cyclosporine A. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation-related index was determined by carboxyl fluorescin diacetate succinmidyl ester flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以Con A作为T细胞活化、增殖的刺激剂，以不同浓度的TMB-8及与环孢菌素A联合作用于该系统，用流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术，分析TMB-8在Con A刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色分析细胞周期的分布情况。

Taking the Wuhan Theater as core, based on the characteristic bars and performance places, a high-level recreational and cultural center will be built; on the plot opposite to the Fu-mart there will be a playground for freestyle bike and hip-hop dance; by the Jianghan road and the Music King Performance Hall a gardenesque underground parking lot is planed; the Huangshi road will be widened and an outdoor gallery will be set on its eastern side, with a plaza at the road mouth for street artists.

A、激越飞扬，演艺天堂——夜汉口激越飞扬区以武汉剧院为核心景观，作为标志性的文化活动场所，利用其文化的辐射力，整合区域内酒吧、演艺场所，展现大汉口夜的魅力。拟在江汉路口、大福源对面临街区建设青少年花样自行车运动场和街舞活动场。轻轨江汉路站附近和音乐王对面配置园林式地下停车场或多层停车楼。按规划拓宽黄石路，在黄石路口东侧结合低层建筑拆迁，扩大建设黄石路画廊，路口拐角处开辟小型街头画家广场。

Soybean is a good dietary protein resource.11S and 7S (p-conglycinin) are major components in soybean. Glycinin is consisted of acidic subunits and basic subunits, Acidic and basic subunits formed five complexes: group I (A1aB2, A1bB1b,A2B1a) and II(A5A4B3, A3B4), P-conglycinin is consisted of a', a, and p subunits.

大豆蛋白质是优质的植物蛋白源，其主要组分为11S和7S(β-conglycinin)。11S是由酸性亚基A和碱性亚基B构成的，A和B形成五种复合体：ⅠA_(2aB_2，A_(1b)B_(1b)，A_2B_(1a)和Ⅱ(A_5A_4B_3、A_3B_4)；7S(β-conglycinin)是由α'，α和β三个亚基构成的。

Vegetable blood group A Japanese scientist studied a variety of 500 angiosperm and gymnospermous seed and fructification, discovery has O blood group 60 kinds among them, 24 kinds have B blood group, write a few plants additionally to have AB blood group, but did not find namely can deciding is A model plant.

植物血型一位日本科学家研究了500多种被子植物和裸子植物的种子和果实，发现其中60种有O型血型，24种有B型血型，另写一些植物有AB型血型，但就是没有找到能够判定是A型的植物。

In the biological functionaddition, both biochanin A and hesperitin might inhibited the colony -formation forming ability of K562 cells through up-regulated Notch signaling. Besides,The treatment with biochanin A and hesperitin enhanced the erythroid-differentiation of K562 cells. It is important to investigate Tthe molecular mechanisms of biochanin A and hesperitin to regulate the endogenous CBF1-dependent Notch signaling will be further investigated.

在生物功能方面，biochanin A与hesperitin可能经由Notch讯息传导的活化，抑制了K562细胞群落的形成，除此之外，这两种药物均明显促进K562细胞往红血球分化。biochanin A与hesperitin对於CBF1所媒介之内生性Notch讯息传导的分子调控机制仍有许多问题尚未厘清，需要更多的实验与研究来讨论。

The results showed that: three major peaks,humuslike fluorescence peak A,proteinlike fluorescence peaks B and C appeared on the threedimensional fluorescence spectroscopy;the DOM fluorescence intensity was well related with DOC and CODMn,indicating that fluorescence dissolved organic matter content can reflect both the organic carbon content and the organic matter content by oxidation in the water in August,during the nonalgae bloom outbreak period,fluorescence intensity had a poor correlation with chlorophyll a,indicating that phytoplankton was not the main source of the fluorescent substances.

结果显示：小江回水区DOM的三维荧光光谱主要表现出3个峰，类腐殖质荧光峰A、类蛋白荧光峰B和C；DOM荧光强度与DOC、CODMn相关性较好，表明荧光溶解有机物质的含量可以较好反映水体中有机碳和可被氧化有机物的含量；在8月份非水华暴发时期荧光强度和叶绿素a相关性较差，表明DOM荧光物质主要不是由浮游植物产生。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。