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I'll just play a little bit to see how it feels...Three hours later, I realized I was addicted. Which is awful, because I really thought I was done playing this game in 1990. The thing is, they put in just enough new features compared to the original game that all my old reflexes from the Apple II, two-button joystick are worse than useless.Just when I think I've got it wired, I hold the A button pressed a half-second too long and die a horrible death. It makes me want to kill whoever designed it.

我觉得我会再看看手感如何。。。3个小时后我被吸引住了，就像90年时的感觉一样，简直无语了，他们放了足够多的新元素来对比我当初在Apple2机上的感觉，那两个摇杆不如不要的好，当我感觉我已经上手时，我多压了A半秒，结果导致一场可怕的死亡。

Another benefit to Meguiar's A-12 Cleaner Wax is it's widespread availability, and affordable price,(suggested retail is $4.95). This makes it another good choice for people with daily drivers that would like to wax their car often. And if you like to see water beading on your car's finish after washing or following a rain storm, you will not be disappointed. The chemists at Meguiar's recently reformulated A-12 Cleaner Wax to bead water like nobody's business! Water beads are small, tall and tight and last and last. Water beading, as well as darkness gets better and better with each additional coat. Easy to use, Meguiar's Cleaner Wax wipes on easy, dries fast, and removes effortlessly with a premium quality 100% cotton towel, or Meguiar's own microfiber polishing cloth, the Ultimate Wipe. Meguiar's Cleaner/Wax can be applied by hand, dual-action polisher and orbital buffers.

美克拉A-12清洁蜡的其他优势是产品随处可得和价格是人人负担得起的（建议售价是4.95美金），这使得它成为天天开车且喜欢经常为他们的爱车上蜡的人们另一个好的选择，如果你喜欢在洗完车或大雨过后看到水珠挺立在你的烤漆上，你将不会失望的，美克拉的化学家最近为了让水可以在漆面上形成水珠而重新调配A-12清洁蜡的配方，现在水珠会变得更小、直挺、紧凑、持久的，烤漆在你每多上一层涂层会变成越来越深沈，它是容易使用的，美克拉清洁蜡上蜡容易、快乾且使用一条优质的100%纯棉毛巾或美克拉生产的超细纤维布来下蜡是毫不费力的，美克拉清洁蜡可以使用手、震抛机和打蜡机来施用。

Another benefit to Meguiar's A-12 Cleaner Wax is it's widespread availability, and affordable price,(suggested retail is $4.95). This makes it another good choice for people with daily drivers that would like to wax their car often. And if you like to see water beading on your car's finish after washing or following a rain storm, you will not be disappointed. The chemists at Meguiar's recently reformulated A-12 Cleaner Wax to bead water like nobody's business! Water beads are small, tall and tight and last and last. Water beading, as well as darkness gets better and better with each additional coat. Easy to use, Meguiar's Cleaner Wax wipes on easy, dries fast, and removes effortlessly with a premium quality 100% cotton towel, or Meguiar's own microfiber polishing cloth, the Ultimate Wipe. Meguiar's Cleaner/Wax can be applied by hand, dual-action polisher and orbital buffers.

美克拉A-12清洁蜡的其他优势是產品随处可得和价格是人人负担得起的（建议售价是4.95美金），这使得它成為天天开车且喜欢经常為他们的爱车上蜡的人们另一个好的选择，如果你喜欢在洗完车或大雨过後看到水珠挺立在你的烤漆上，你将不会失望的，美克拉的化学家最近為了让水可以在漆面上形成水珠而重新调配A-12清洁蜡的配方，现在水珠会变得更小、直挺、紧凑、持久的，烤漆在你每多上一层涂层会变成越来越深沉，它是容易使用的，美克拉清洁蜡上蜡容易、快乾且使用一条优质的100%纯棉毛巾或美克拉生產的超细纤维布来下蜡是毫不费力的，美克拉清洁蜡可以使用手、震抛机和打蜡机来施用。

Bleach主题曲 life is like a boat 歌曲:Life Is Like A Boat 歌手:Rie Fu Life Is Like A Boat 演唱: Rie fu 词/曲: Rie fu Nobody knows who I really am I never felt this empty before And if I ever need someone to come along Who's gonna comfort me, and keep me strong We are all rowing the boat of fate The waves keep on comin' and we can't escape But if we ever get lost on our way The waves would guide you thru another day 远くで息をしてる透明になったみたい暗暗に思えたけど目隠しされてただけ祈りをささげて新しい日を待つ鲜やかに光る海その果てまで Nobody knows who I really am Maybe they just don't give a damn But if I ever need someone to come along I know you would follow me, and keep me strong 人の心はうつりゆく抜け出したくなるつきはまた新しい周期で舟を连れてく And every time I see your face The oceans heave up to my heart You make me wanna strain at the oars And soon I can see the shore Ah, I can see the shore ...

life is like a boat http：//www.cg-ring.com/music/riefu01.wma 刷吧我认了 Life is like a boat 歌词大意：没有人知道我究竟是谁我以前从未感觉到如此虚无空荡如果我需要有人来陪伴谁会安慰我并让我更加坚强我们都在命运之湖上荡舟划桨波浪起伏这而我们无法逃离孤航但是假使我们迷失了方向波浪将指引着我们穿越另一天的曙光在遥远的地方呼吸着仿佛变成了透明一般还以为是周遭的黑暗却只是被蒙住了双眼虔诚地祈祷着期待新的一天的到来直到闪耀着亮丽光芒的大海边际没有人知道我究竟是谁或许他们并不会指责我的荒唐但如果我需要有人来陪伴我知道你会追随我并使我坚强想从不断变迁的人心中挣脱又一轮阴晴圆缺的月亮牵引着我向前每一次我看见你的脸庞海洋奋力托起我的心脏你令我在荡桨时感到紧张转眼我竟看不到岸的彼方啊，我看不到岸的彼方。。。。。。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

To obtain a basal concentration of serum amyloid A in cattle in Taiwan, SAA concentrations were determined from serum samples collected from 10 healthy heifers, once a week for 10 weeks.

为测定台湾牛只急性期蛋白血清淀粉样蛋白A (serum amyloid A， SAA)的血清浓度，本实验采集血清的对象为10头健康女牛，每周固定时间采样一次，共10周。

The results are as follows: the DDM detergent dissolves the Cyt b6f better thanβ-OG detergent does; DDM can keep the Cyt b6f in native state, under which the hemes and Chl a experience specific environment, while theβ-OG make the complex in denatured state in some sort, under which the catalyzed electron transfer activity of the complex

结果表明，DDM去垢剂能使Cyt b6f处于较好的分散体系中，其中血红素和Chl a分子处于特定的蛋白环境中，不会导致Cyt b6f变性；而β-OG去垢剂会使Cyt b6f产生聚合现象，其中的血红素和Chl a与蛋白环境的相互作用减弱，和DDM相比其电子传递活性显著降低，Chl a单线激发态寿命延长，Chl a更容易被光

Total RNAs from KAx-3 cells and AK127 cells(developed for 14h) were isolated. After the reverse transcription and PCR reaction, two distinct differential fragments were acquired., fragment A was from KAx-3 cells and fragment C was from AK127 cells. After retriving and reamplifying the differentially expressed fragments, white-blue plaqueselection, the fragments were purified. Northern blot proved that fragment A was from KAx-3 cells and fragment C was from AK127 cells. The results of sequencing and researching for NCBI database have been showed: part sequence of fragment A shows 91% similarity to the gene encoding DhkA, 92% similarity to the gene encoding DhkF, 91% similarity to the gene encoding STATc, 97% similarity to the homoeobox gene encoding protein. These genes play important part in controlling cell differentiation and cell proportion in Dictyostelium discoideum.

本研究通过提取盘基网柄菌发育14小时的野生型KAx-3细胞和突变型AK127细胞的总RNA，运用mRNA差异显示法分离出了两条明显的差异表达片段，其中片段A来自野生型KAX-3细胞，片段C来自突变型Ak127细胞；并通过凝胶回收差异片段、对差异片段进行再次PCR、蓝白斑筛选克隆、提取质粒、酶切电泳纯化差异片段；接着进行Northern杂交的结果表明，片段A只与野生型KAx-3细胞的总RNA有杂交信号，片段C只与突变型AK127细胞的总RNA有杂交信号，这就排除了差异片段假阳性的可能；最后通过测序，搜索NCBI BLAST数据库发现：片段A的小部分序列与编码组氨酸激酶DhkA基因中一段序列的相似性高达91%，与编码组氨酸激酶DhkF基因中的一段序列相似性高达92%，与编码STATc蛋白基因的一段序列相似性达91%，以及与编码同源框蛋白的基因中的一段序列相似性达97%，这些基因在盘基网柄菌细胞分化和细胞比例调控过程中起着相当重要的作用，这些数据进一步说明了突变细胞不能完成发育的原因。

Results: by the end of 4 weeks, there was significant difference in efficacy between A、C groups and B groups（P＜0.05）,by the end of 6 weeks, there was significant difference in efficacy between c group and A、B groups ,there was no significant difference in efficacy between A and B group(P＞0.05),and the doses of imipramine and the TESS scores of C group were significantly lower than that of B group.

结果：三组病人在治疗后4周时，A、C两组减分与B组减分差异有显著性（P＜0.05）；治疗6周时A、B两组差异无显著性（P＞0.05），C组效果显著优于A、B两组（P＜0.01），且C组丙咪嗪剂量和TESS评分明显低于B组。

The results show that coal and gangue are involved in the microbial role; the quantity of inoculums is directly related to the quantities of gas and the contents of methane, at the same time it has a certain degree of impact on the contents of NO(superscript - subscript 3)-N, NH(superscript + subscript 4)-N, S(superscript 2-), SO(superscript 2- subscript 4), and metals; moreover, because of the microbial anaerobic fermentation, the contents of NH(superscript + subscript 3) are seriously substandard; overall, the group B which has a smaller proportion of gangue has a less impact to the environment compared to the group A which has a larger proportion of gangue.

结果表明：煤、煤矸石参与了微生物作用；接种物的加入量与产气量和甲烷含量密切相关，并对硝酸盐氮、氨氮、硫化物、硫酸盐和金属也有一定程度的影响；同时由于微生物厌氧发酵的关系，氨氮严重超标；矸石比例较小的B组对环境的影响略小于矸石比例较大的A组。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。