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Results: Twenty one differential protein spots were screened and 5 differential proteins were identified. They included Ezrin protein, transcription elongation factor A, and lamin A/C which were up-regulated in LoVo cells and non-metastatic protein expressed in isoform a (NM23 A) and heat shock protein 8 (HSPA8) which were up-regulated in SW480 cells.

结果:筛选出21个差异蛋白质点,成功鉴定出5种差异蛋白质,其中在LoVo细胞株中表达上调的蛋白有埃兹蛋白、转录延长因子A (transcription elongation factor A)和甘氨酸A/C,在SW480细胞株中表达上调的蛋白有NM23蛋白[non-metastatic cell 1 protein (NM23A) expressed in isoform a]和(heat shockprotein 8,热休克蛋白8)。

These compounds were identified as follows:chrysophanol (FP-1),physcion(FP-2),eriosematin(FP-3),scoparone(FP-4),lupeol(FP-5),betulinic acid(FP-6),3\',4\'-Dihydroxy-trans-cinamic acid octacosyl ester(FP-7),β-sitosterol (FP-8),flemiphilippinone A(FP-9),monopalmitin(FP-10),emodin(FP-11),islandicin (FP-12),salicylic acid(FP-13),p-methoxyphenylpropionic acid(FP-14),trideca-1, 3-diene(FP-15),lupinifolin(FP-16),flemichin D(FP-17),flemiphilippinin A(FP-18), auriculasin(FP-19),erythrinin B(FP-20),6-C-prenylluteolin(FP-21), 8-(1,1-dimethylallyl) genistein(FP-22),flemiphilippinin E(FP-23),flemiphilippinin F (FP-24),5,7,3\',4\'-tetrahydroxy-6,8-diprenylisoflavone(FP-25),flemiphilippinin D (FP-26),dorsmaninsⅠ(FP-27),osajin(FP-28),6,8-diprenyleriodictyol(FP-29), lupinalbin A(FP-30),genistein(FP-31),3\'-O-methylorobol(FP-32),orobol(FP-33), 5,7,2\',3\',4\'-pentahrdroxyflavone(FP-34),the mixture of biochanin A and prunetin (FP-35 and 36),genistin(FP-37),sophororicoside(FP-38),3\'-O-methylorobol-7-glucoside(FP-39),the mixture of sissotrin and prunetin 4\'-O-β-D-glucoside(FP-40 and 41),adenosine(FP-42) and luteoloside(FP-43,mixture).

这些化合物分别为大黄酚(FP-1)、大黄素甲醚(FP-2)、eriosematin(FP-3)、滨蒿内酯(FP-4)、羽扇豆醇(FP-5)、白桦酸(FP-6)、咖啡酸二十八烷酯(FP-7)、β-谷甾醇(FP-8)、蔓性千斤拔酮A(FP-9)、单棕榈酸甘油酯(FP-10)、大黄素(FP-11)、islandicin(FP-12)、水杨酸(FP-13)、对甲氧基苯丙酸(FP-14)、十三烷-1,4-二烯烃(FP-15)、lupinifolin(FP-16)、千斤拔素D(FP-17)、蔓性千斤拔素A(FP-18)、auriculasin(FP-19)、erythrinin B(FP-20)、6-C-异戊烯基木犀草素(FP-21)、8-(1,1-二甲烯丙基)-染料木黄酮(FP-22)、蔓性千斤拔素E(FP-23)、蔓性千斤拔素F(FP-24)、5,7,3′,4′-四羟基-6,8-双异戊烯基异黄酮(FP-25)、蔓性千斤拔素D(FP-26)、dorsmaninsⅠ(FP-27)、osajin(FP-28)、6,8-双异戊烯基圣草素(FP-29)、lupinalbin A(FP-30)、染料木黄酮(FP-31)、3\'-O-甲基香豌豆苷元(FP-32)、奥洛醇(FP-33)、5,7,2′,3′,4′-五羟基黄酮(FP-34)、鹰嘴豆素甲和樱黄素的混合物(FP-35和FP-36)、染料木苷(FP-37)、槐属苷(FP-38)、7-葡萄糖基-3\'-O-甲基香豌豆苷(FP-39)、印度黄檀苷和樱黄素4′-O-β-D-葡萄糖苷的混合物(FP-40和FP-41)、腺嘌呤核苷(FP-42)和木犀草苷(FP-43,混合物)。

Methods Activate groups A and C meningococcal polysaccharide with cyanogens bromide and prepare into MenAPS-ADH and MenCPS-ADH derivatives by reacting with 1, 6-adipic acid dihydrazine. Mix MenAPS-ADH and MenCPS-ADH derivatives with purified tetanus toxoid at a certain mass ratio, then add l-ethyl-3(3-dimethylaminopropyl)-carbodiimide to prepare bulks of MenAPS-TT and MenCPS-TT conjugates respectively. Mix the two bulks at a certain ratio, add stabilizer and lyophilize to prepare group A+C meningococcal polysaccharide conjugate vaccine.

A群和C群脑膜炎球菌多糖在溴化氰的活化下,以1,6己二酰肼作为连接子,与精制破伤风类毒素在碳二亚胺作用下结合,再按一定的比例将两者混合,制成A+C群脑膜炎球菌多糖结合疫苗原液,加保护剂冻干后,制成A+C群脑膜炎球菌多糖结合疫苗。

By comparing different processes in the same period, following operting modeof interactive reactor was recommend: enhanced A~2/O process fits on spring period,pre-anoxic inverted A~2/O process was recommend in dry/mizzle weather in summerperiod, step aeration process was adopted in rainy weather in summer period,alternating oxygen concentration inverted A~2/O process was appropriate in autumnperiod, and normal inverted A~2/O process was better in winter period.

通过不同季节多种工艺的比较,确定了高效生物反应器运行模式:春季时段采用改良型A~2/O工艺,夏季晴天/小雨时段采用预缺氧+倒置A~2/O工艺,夏季连续暴雨时段采用多点进水工艺,秋季时段采用低氧/常氧交替运行的倒置A~2/O工艺,冬季时段采用常规倒置A~2/O工艺。

Unlike previous models in which overexpression of the amyloid precursor protein results in amyloid plaque formation, have produced mice that overexpress only Aβ40 or Aβ42 and prove that Aβ42 is critical for the formation of amyloid deposits in vivo.

不同于早先调控淀粉样蛋白前体蛋白的动物模型,McGowan 等(Neuron, Vol 47, 191-199, 21 July 2005)培养了专一表达Aβ40或者 Aβ42的老鼠,结果直接证明了Aβ42 确实是引起淀粉样蛋白沉淀的主要因素,而不是Aβ40。

Successful separation of Cd-pheophytin a, Cd-pheophytin b, chlorophyll a, chlorophyll b, pheophytin a and pheophytin b in 14min was firstly accomplished by reversed-phase HPLC at a flow rate of 0.8ml/min, with the mobile phase of methanol: acetone (80: 20, v/v).

首次以甲醇:丙酮=80:20为流动相,流速为0.8ml/min,在C〓柱(5μm,4.0×250mm)上,14min内成功实现了镉叶绿素a和镉叶绿素b配合物与叶绿素a,b和脱镁叶绿素a,b的高效液相色谱分离。

The absorption bands observed for Pb-pheophytin a at 462nm and 673nm , while for Pb-pheophytin b at 480nm and 662nm . Compared with pheophytin a and pheophytin b, all these bands red shifted more or less. Among the reported metallochlorophylls, the degree of red shift of Pb-pheophytin in Soret band was the largest by 54nm and 44nm for Pb-pheophytin a and Pb-pheophytin b, respectively.

实验表明:对铅叶绿素a和b系列来说,它们在Soret带、Q带的特征吸收峰分别在462nm,673nm和480nm,662nm;与脱镁叶绿素a,b相比,铅叶绿素a,b在Soret带和Q带吸收峰均有不同程度的红移;与现已报道的金属叶绿素相比,铅叶绿素a,b在Soret带的红移程度最大,分别为54和44nm;铅叶绿素不具有荧光活性,并能猝灭脱镁叶绿素的荧光。

The working principle and the structure of a hybrid magnetic bearing are investigated. The hardware of a digital controller system based on a fixed poind digital singal processor TMS320C25, as well as an A/D and D/A converter is presented.

研究了混合磁悬浮轴承系统的工作原理及其控制器,讨论了以定点数字信号处理器TMS320C25为核心的数字控制硬件系统的构成以及A/D,D/A等外围电路的设计。

An 8-bit counter with a 7-bit software programma- ble prescaler, a digital hardware activated watchdog function, a 14-bit voltage syn- thesis tuning peripheral, a Serial Peripheral Inter- face, up to six 6-bit PWM D/A converters, up to 7 8-bit A/D Converter, an on-screen display with 20 characters per line and 192 charac- ters.

一个8位7位规划中的软件,竹叶提取预分频器,一个数字硬件看门狗功能激活,14位电压的SYN -论文调谐外设,一个串行外设间面的,多达6个6位PWM式D / A转换器,多达7 8位A / D转换器,屏幕上显示每行20和192 charac - TER值在一家银行字符的。

The present research situation about AB process in and out of our country was analyzed in this paper. In addition to a concentrated study of the factors affecting sedimentation performance of A-step activated sludge, the rule of microbe multiplication, yield of activated sludge in A-step and treatment of low-concentration sewage were also involved in this paper. The main conclusions are as follows:(1) The study on factors affecting sedimentation performance of A-step activated sludge shown that the inflow SS distinctly improve sedimentation performance of activated sludge, the less influent SS makes the sludge bulking easy, and the pythogenous sewage evidently worsen sedimentation performance of activated sludge.

本文分析了国内外AB工艺的研究现状,对A段活性污泥沉降性能的影响因素;A段的微生物增殖规律、活性污泥产量以及A段工艺处理低浓度城市污水等问题进行了试验研究,得出了以下结论:(1)对A段活性污泥沉降性能影响因素的研究表明:进水中的SS对污泥沉降性能有明显的改善作用,如果进水中SS较少,易导致污泥膨胀;而腐化污水对活性污泥的沉降性能有显著的不良影响。

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