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Study on the Crystallization Process of Labeled GL-7-ACA Acylase CA130 Complex 7β-bromoacetyl amino cephalosporanic acid (BA-7-ACA), an analog of glutaryl-7-amino cephalosporanic acid (GL-7-ACA), can inhibit and specifically alkylate GL-7-ACA acylase (CA130) from Pseudomonas sp. 130, forming a carbon-carbon bond between BA-7-ACA and the C-2 on indole ring of Trp-β4 residue of CA130.Here we reported that BA-7-ACA labeled CA130 (BA-C130) could self-catalyze the hydrolysis of BA-7-ACA during crystallization process. The hydrolysis was confirmed to be a reaction analogous to the one of GL-7-ACA by comparative MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) spectrometry analysis.

二、GL-7-AcA酰化酶CA130标记复合物的结晶过程研究溴乙酰氨基头孢烷酸（7β-bromoacetyl amino cephalosporanic acid，BA-7-ACA）作为戊二酰-7-氨基头孢烷酸（GL-7-ACA）的类似物，不仅能够抑制GL-7-ACA酰化酶CA130的活力，而且能通过在BA-7-ACA和CA130的β亚基第四位色氨酸吲哚环二位碳原子之间形成碳-碳共价键而将CA130特异的烷基化。

Determined the core species and the species concerned, the core species contains 8 species of fishes, 7 species of amphibians, 3 species of reptiles, 7 species of mammals, 7 species of birds, 10 species of plants, and 7 types of plant communities, totally 38 species and 7 types of communities. Four species of waterfowls: Siberian Crane, Oriental White Stork, Lesser White-fronted Goose and Chinese Merganser are among the 7 species of core bird species.

确定了核心物种与受关注物种，其中核心物种分别包括鱼类8种、两栖类3种、爬行类3种、兽类7种、鸟类7种、植物10种，植物群落7种，共38个物种与7种群落。7种鸟类核心物种中，水禽有4种，即白鹤、东方白鹳、小白额雁、中华秋沙鸭。4。

They are 4-(2- hydroxy-3-butynlenoxy) benzoic acid (1, WA), 5-chloro-7, 8-dihydroxy-7-methyl- 6-oxo-3- [ -3, 4-dihydroxy-3, 5-dimethyl-l-heptylene] -1H-8, 8a-dihydrobenzo [2, 3-c] pyran (2, WB),-2-(2-methyl-2-dibutene diamido)-2-butenoic acid (3, B5262), 3, 4-dihydro-9, 10-dihydroxy-7-methoxy-3-methyl-1H-naphtho [2, 3- c] pyran-1-one (4, A73 semi-Vioxanthin), 8, 8'-bis (6, 9-dioxo-3,4-dihydro-10- hydroxy-7-methoxy-3-methyl-1H-naphtho [2, 3-c] pyran-1-one)(5, A122 Xanthomegnin), 2, 5-dioxo-3a-hydroxymethyl-3, 3a, 6, 6a-tetrahydro-furo [2, 3-b] furan (6, 1003-2), 7-acety1-5-chloro-6, 8-dioxo-7-methyl-3- [ -3, 5-dimethyl-1, 3- diheptylene] -4aH-benzo [2, 3-c] pyran (7, M2-2 sclerotiorin), respectively.

它们分别被命名为4-（2-羟基-3-丁炔氧基）苯甲酸（1，WA）、5-氯-7，8-二羟基-7-甲基-6-氧代-3-[-3，4-二羟基-3，5-二甲基-1-庚烯基]-1H-8，8a-二氢苯并[2，3-c]吡喃（2，WB）、-2-（2-甲基-2-丁烯二酰亚胺基）-2-丁烯酸（3，B5262）、3，4-氢-9，10-二羟基-7-甲氧基-3-甲基-1H-萘并［2，3-c］吡喃-1-酮（semi-Vioxanthin，4，A73）、8，8'-双（6，9-二氧代-3，4-二氢-10-羟基-7-甲氧基-3-甲基-1H-萘并［2，3-c］吡喃-1-酮）（Xanthomegnin，5，A122）、2，5-二氧代-3a-羟甲基-3，3a，6，6a-四氢-呋喃并［2，3-b］呋喃（6，1003-2），7-乙酰基-5-氯-6，8-二氧代-7-甲基-3-［-3，5-二甲基-1，3-庚二烯基］-4aH-苯并2，3-c］吡喃（Sclerotiorin，7，M2-2）。

The research investigates the electromagnetic radiation intensity of MRI in some hospitals of southern Taiwan involving: Chung-Ho Memorial Hospital, Kaohsiung Medical University, Kaohsiung Verterans General Hospital, Chang-Gung Memorial Hospital Department of Kaohsiung, Chi Mei Medical Center Department of Tainan, Kaohsiung Municipal Hsiaokang Hospital, 802 Kaohsiung Armed Forces General hospital and Yuan's General Hospital etc. Finally, we got the result On the intensity of electromagnetic radiation was between 6.7μT to 7.8μT. Among these tests, the intensity of electromagnetic radiation of the head is ranged from 7.2μT to 7.6μT, the chest from 7.3μT to 7.7μT, the abdomen from 7.3μT to 7.8μT, the center of door from 6.9μT to 7.5μT, and the relater of control room from 6.7μT to 7.4μT.

本研究调查台湾南部几所医院－高雄医学大学附设中和纪念医院、高雄荣民总医院、长庚纪念医院高雄院区、奇美医院台南院区、高雄市立小港医院、802国军高雄总医院、阮综合医院等几所医院内，MRI作业场所电磁辐射强度，测量结果磁场强度范围介於6.7μT到7.8μT，其中头部磁场值范团7.2μT到7.6μT，胸部磁场值范围7.3μT到7.7μT，腹部磁场值范围7.3μT到7.8μT，门中央磁场值范围6.9μT到7.5μT，控制室中央磁场值范围6.7μT到7.4μT。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

Undefeated . Brian faced and defeated the following competitors TF Whittington 7-5; David English 7-0; Ron Gilbert 7-3; Lance Ireland 7-4; Pat Cooper 7-4; David Tresh 7-5; in the finals Tyler defeated Jason Steman 7-3 taking 1st place in the Amateur Event.

布赖恩面对和战胜了以下的竞争对手的TF惠廷顿7月5日；国宝英语7-0 ；罗恩吉尔伯特7月3日；兰斯爱尔兰7月4日；帕特库珀7月4日；国宝tresh 7月5日，在决赛中战胜了泰勒贾森steman 7月3日以第一的地方，在业余活动。

Their chemical structures were characterizd on the basis of spectral and chemical evidences to be: Sarracenin (1), 2-methyl-5-hydroxy-7-O-ethyl caffeate chromome (2), Naringenin(3), 2-methyl-5, 7-dihydroxychromome (4), 2-methyl-6-hydroxyl-cinnamic acid methyl ester (5), eriodictyol (6), quercetin (7), Naringenin-7-O-β-D-glucoside( 8 ), eriodictyol-7-O-α-D-glucoside (9), quercetin-3-O-β-D-glucoside(10). In addition, one compound was abtained from the n-butanol frationk, which was finally identified as Morrosiside(11) by spectral and chemical evidences.

以波谱数据及其理化性质鉴定为：Sarracenin(1)，2-甲基-5-羟基-7-O-咖啡酸乙酯色原酮(2)，柚皮素(3)，2-甲基-5，7-二羟基色原酮(4)，2-甲基-6-羟基苯丙烯酸甲酯(5)，圣草酚(6)，槲皮素(7)，柚皮素-7-O-β-D-葡萄糖苷(Naringenin-7-O-β-D-glucoside)(8)，圣草酚-7-O-α-D-葡萄糖苷(eriodictyol-7-O-α-D-glucoside)(9)，槲皮素-3-O-β-D-葡萄糖苷(quercetin--3-O-β-D-glucoside)(10)；另外从正丁醇萃取部位分离得到1个化合物，以波谱数据和文献对照鉴定为：Morroniside(11)。

To study the bioactive components from water soluble extract of the Chinese eaglewood, various column chromatography of Chinese eaglewood led to the isolation of ten compounds. Their structures were elucidated as 2,3-bis(3-hydroxyphenyl)- propan-1-ol (1), [(2R,3R)-3-(4-amino-3,5-dimethoxyphenyl)oxiran-2-yl]methanol (2), 8-chloro-4'- methoxy-5,6,7,3'-tetrahydroxy-2-(2-phenethyl)-5,6,7,8-tetrahydrochromone (3), 4'-methoxy-5,6,7,3'-tetrahydroxy-2--5,6,7,8-tetrahydrochromone (4), the isomer of 4 (5), 4'-methoxy-5,6,7,8-tetrahydroxy-2-(3'-hydroxyphenethyl)-5,6,7,8- tetrahydrochromenone (6), 4'-methoxyisoagarotetrol (7), 6-hydroxy-2-(2-hydroxy-2- phenethyl)-chromone (8), 6-hydroxy-2-(2-phenethyl)-chromone (9), 6-hydroxy-2-(4'- hydroxy-2-phenethyl)-chromone (10), and Guaiacylacetone (11) by means of spectral analysis and comparison with references.

为了进一步发现沉香中具有生理活性的化学成分，本文对国产沉香的水溶性成分进行了研究，从中分离得到11个化合物，通过红外、紫外、质谱、核磁共振(1H-NMR， 13C-NMR， COSY， HSQC， HMBC， ROESY)波谱解析，分别鉴定为：2，3-二(3-羟基苯基)-丙-1-醇(1)，[(2R，3R)-3-(4-氨基-3，5-二甲氧苯基)-2-氧化烯基]甲醇(2)，8-氯-4'-甲氧基-5，6，7，3'-四羟基-2-(2-苯乙基)-5，6，7，8-四氢色原酮(3)，4'-甲氧基-5，6，7，3'-四羟基-2-(2-苯乙基)-5，6，7，8-四氢色原酮(4)，化合物3的差向异构体(5)，4'-甲氧基-5，6，7，8-四羟基-2-(3'-羟基-2-苯乙基)-5，6，7，8-四氢色原酮(6)，4'-甲氧基异沉香四醇(7)，6-羟基-2-(2-羟基-2-苯乙基)色原酮(8)，6-羟基-2-(2-苯乙基)色原酮(9)，6-羟基-2-(4'-羟基-2-苯乙基)色原酮(10)和Guaiacylacetone (11)。

Their structures were characterized by IR, 1H NMR, of which 4-chloro- 2-methyl-7-(2-bromoethoxy) isoflavone, 2, 4\'-dimethyl-7-(2-bromo-ethoxy)isoflavone and 2-methyl-7-(2-bromoethoxy) isoflavone are rarely reported so far.4\'-chloro-2-methyl-7-(3-bromopropoxy)isoflavone, 2, 4\'-dimethyl-7-(3-bromo- propoxy) isoflavone, 2-methyl-7-(3-bromopropoxy) isoflavone, 4\'-methoxy-2-methyl-7-(3-bromopropoxy) isoflavone and 4\'-hydroxy-2-methyl-7-(3-bromopropoxy) isoflavone were gained by reacting 7-hydroxy-2-methyl isoflavone derivatives with 1, 3-dibromo- propane, respectively.

第三章(来源：73ABC论文网www.abclunwen.com)在丙酮溶液中以K_2CO_3为碱，使溴乙氧基异黄酮和溴丙氧基异黄酮与咪唑偶合，以较高的产率合成出10种目标化合物：2-甲基-4′-氯-7-2-(1-咪唑基乙氧基异黄酮、2，4′-二甲基-7-2-(1-咪唑基乙氧基异黄酮、2-甲基-7-2-(1-咪唑基乙氧基异黄酮、2-甲基-4′-甲氧基-7-2-(1-咪唑基乙氧基异黄酮、2-甲基-4′-羟基-7-2-(1-咪唑基乙氧基异黄酮、2-甲基-4′-氯-7-3-(1-咪唑基丙氧基异黄酮、2，4′-二甲基-7-3-(1-咪唑基丙氧基异黄酮、2-甲基-7-3-(1-咪唑基丙氧基异黄酮、2-甲基-4′-甲氧基-7-3-(1-咪唑基丙氧基异黄酮、2-甲基-4′-羟基-7-3-(1-咪唑基丙氧基异黄酮，经IR、~1H NMR、~(13)C NMR、元素分析等对其结构进行了表征，10种目标产物均未见文(来源：ABC论文cccccc网www.abclunwen.com)献报道。

Specialized introduction: This specialized raise has the electronic technology and the information system elementary knowledge, can be engaged in each kind of electronic installation and the information system research, the design, the manufacture, the application and the development higher project technology talented person Main curriculum: The signal and the system, the digital signal processing, the correspondence principle, the microcomputer principle and the connection technology, the monolithic integrated circuit principle and the application technology, the DSP technology and the application, the EDA technology, the construction of data, embedded Linux using the programming, the feeling measure technical, the electric circuit theory series curriculum, the computer technology series curriculum and so on individual hobby * software and hardware research and development computer correspondence literature ping pong photography The experience and the personal experience * 2006.6.29-7.8 compile the staff management system management system using the C language, its basic service activity includes: The staff information warehousing, the revision, the inquiry, the insertion, deletes the staff information and so on * 2006.7.10-7.20 monolithic integrated circuit curriculum design period completes the stopwatch the design, namely the initialization timer is 99:99, 0 starts using the monolithic integrated circuit timer fixed time, fixed time arrives when 0s to transmit the signal to cause the buzzer bell, and may realize suspends, the continuation and the replacement function * 2007.7.2- 7.8 practises in Luoyang Big dragon Peony Communication facility Limited company, does has liquid crystal display monitor telephone one, simultaneously visited has served under somebody's banner the correspondence company, and studied has simulated the telephone the design electric circuit, had understood its basic principle * 2007.7.4- 7.18 completed the DSP curriculum in the school to design, realizes the FIR numeral filter, namely transmitted 25 from DSPThe height level, after advocates AC01 D/A to transform the simulation square-wave, then passes to again from AC01 carries on A/D to transform, produces the data signal, after the DSP numeral filter, finally produces the sine wave * 2008.8.1-8.15 to practise in the Zhejiang Jiang hua abundant power tool limited company, studies the computer software and hardware maintenance.

专业介绍：本专业培养具备电子技术和信息系统的基础知识，能从事各类电子设备和信息系统的研究、设计、制造、应用和开发的高等工程技术人才主要课程：信号与系统、数字信号处理、通信原理、微机原理与接口技术、单片机原理与应用技术、DSP技术及应用、EDA技术、数据结构、嵌入式Linux应用编程、感测技术、电路理论系列课程、计算机技术系列课程等个人爱好＊软硬件研发计算机通信文学乒乓球摄影实践经验及个人经历＊ 2006.6.29-7.8 利用C语言编写员工管理系统，其基本业务活动包括：员工信息入库，修改、查询、插入、删除员工信息等＊ 2006.7.10-7.20 单片机课程设计期间完成秒表的设计，即初始化定时器为99：99，利用单片机定时器0开始定时，定时到0s时发送信号使蜂鸣器响铃，并可实现暂停、继续和复位功能＊ 2007.7.2- 7.8 在洛阳巨龙牡丹通信设备有限公司实习，做有液晶显示屏电话机一部，同时参观了旗下通信公司，并学习了模拟电话的设计电路，懂得了其基本原理＊ 2007.7.4- 7.18 在校完成DSP课程设计，实现FIR数字滤波，即从DSP发送25个高低电平，经主AC01 D/A 转换成模拟方波，然后再传给从AC01进行A/D转换，生成数据信号，经DSP数字滤波，最后生成正弦波＊ 2008.8.1-8.15 在浙江华丰电动工具有限公司实习，学习电脑软硬件维护。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。