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Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

ABSTRACT To study the antibacterial constituents from the fermentation broth of endophytic fungus Penicillium sp. 0935030 from mangrove plant Acrostichum aureurm. The separation procedure was guided by antibacterial bioassay. Seven compounds were isolated and purified by column chromatography, and their structures were identified as cyclo (1), cyclo (2), 1(2Furanyl)1,2ethanediol (3),(3β,4β,22β)olean12ene3,22,23triol (4), mannitol (5), uridine (6), and liquiritigenin (7) by physicochemical and spectral data.

为研究红树林植物卤蕨内生真菌Penicillium sp.0935030发酵物中的抗菌活性成分，采用活性追踪的方法，用多种色谱技术对化合物进行分离纯化，通过理化性质和光谱数据鉴定出7个化合物，分别为：环(1)，环(2)，1呋喃基1，2乙二醇(3)，(3β，4β，22β)12烯3，22，23三羟基齐墩果烷(4)，甘露醇(5)，尿苷(6)和甘草素(7)。

The copper atom is in a planar coordination site of [N_2O_2] and it devites from the mean plane by only 0.80 pm. Byπ-πstacking interactions, a alabastrine structure was obtained.(2)Schiff-base complex [Cu(H_2O)]·H_2O, where H3GS is the 3-carboxyl -salicylidene glycine, was synthesized and characterized by elemental analysis, IR spectra and single-crystal analysis. The crystal belongs to monoclinic system, space group P2(1)/c, a=848.46(3)pm, b=681.54(3)pm, c=1967.16(8)pm,β=95.8210(10)°, Z=4, R_1=0.0279, wR_2=0.0724. The copper atom is in a square-pyramidal field with the base

结果表明该晶系属单斜晶系，空间群P2(1)/c，晶胞参数：a=848.46(3)pm，b=681.54(3)pm，c=1967.16(8)pm，α=90°，β=95.8210(10)°，γ=90°，Z=4，R_1=0.0279，wR_2=0.0724，Cu原子位于轻微变形的四方锥场底心，底面被氮原子、酚氧原子、甘氨酸羧基的一个氧原子和一个水分子氧原子占据，而甘氨酸羧基的另一个氧原子占据相邻分子的锥顶，因而形成一维链状结构；合成了单核双聚配合物Na_2[Cu_2_2]·2H_2O ，铜三核配合物Cu_3_2·5H_2O和铜锌异三核配合物ZnCu_2_2·5H_2O，并用元素分析，IR光谱，电子光谱和磁化率测定对配合物的组

Methods Compound 3 was got via the formation of the amide from 2-chloro-3-amino-4-methyl pyridine and 2-chloronicotinoyl chloride, and then nevirapine was acquired by the aminolysis of 3 with cyclopropylamine in an autoclave, followed by the intramolecular nucleophilic substitution.

以2-氯－3-氨基－4-甲基吡啶为原料，依次与2-氯烟酰氯缩合成化合物3、再与环丙胺高压氨解、最后发生分子内亲核取代反应，成功合成了奈韦拉平。

Fluorescent instruments.the colonies of the specimens yielding growth were identified by the vitek32 instruments and kb method was used for the drug sensitivity tests.results three hundred and thirtyone bacteria strains were isolated from a total of 3104 blood culture specimens,with a positive rate of 10.7%.of the identified bacteria,g+ cocci accounted for 50.3%,g-bacilli accounted for 44.7%,fungi accounted for 3.0%,and anaerobian accounted for 1.1%.the susceptibility rates of g-bacilli to imipenem,amikacin and cefoperazone/sulbactam were high.the susceptibility rate of g+ cocci to vancomycin and imipenem were also high.conclusion g+ cocci prevail over g-bacilli in the blood specimens.the staphylococci are the chief bacteria in the childrens blood specimens.both g+ cocci and g-bacilli are sensitive to imipenem.

结果 在3 104份血液培养标本中分离出病原菌331株，阳性检出率为10.7%。病原菌以革兰阳性需氧球菌居首位（50.3%），革兰阴性需氧杆菌次之（44.7%），真菌占3.0%，厌氧菌占1.1%。血液培养中的g+球菌对万古霉素和亚胺培南较为敏感，g-杆菌对亚胺培南、舒普深、丁胺卡那较为敏感。结论血液培养病原菌以g+球菌为主，g-杆菌次之；儿童血液培养病原菌以葡萄球菌属为主；亚胺培南对g+球菌和g-杆菌均具有较高的敏感率。

F total amount 80-122.5 mil, average 102.3±0.71, continuous 7～10 days, to happen Symptom vasospasm 8 case, anesis post-treatment in 5 days,recovery 52 case,midst incomplete 14 case, weight incomplete 10 case, plants exist 11 case, death 3 case, cerbral infarction 2 case , no happing dropsy of brain.

结果 每日分2～3次行腰穿共排放血性脑脊液总量80～122.5ml，平均（102.3±0.71）ml，连续7～10天，发生症状性血管痉挛8例，均在治疗后5天内缓解；痊愈52例，中残14例，重残10例，植物生存11例，死亡3例；出现脑梗死2例，无脑积水发生。

They were respectively fed with a standard diet, a standard diet containing 3% cholesterol and a standard diet containing 2.5?mg/ atorvastatin plus 3% cholesterol diet. After 12 weeks, aortas were removed under deep anesthetization.

随机将雄性新西兰兔24 只分为3组，每组8只：对照组：饲喂标准颗粒饲料；模型组：饲喂含3%胆固醇的颗粒饲料；他汀组：饲喂3%胆固醇的颗粒饲料4周后再加喂阿托伐他汀2.5？

Methods: The clinical data about 3 female cases of tuberous sclerosis accompanying bilateral and multiple renal angiomyolipomas were reported. Their age ranged 16-26 years. All the cases had typical facial angiofibroma and mental retardation. Of them, 2 were admitted to hospital for severe abdominal distending pain and shock, 1 was admitted to hospital for abdominal distention and epilepsy. Cranticocerebral CT revealed typical calcified cortical tuber and /or subependymal nodules, but no etrarenal organ angioleiomyoma in the 3 cases.

报告3例结节性硬化症伴多发性双肾AML的临床诊治资料。3例均为女性，年龄16~26岁，均有典型的智力低下、面部皮脂腺瘤体症，2例因腰部剧烈胀痛伴休克入院，1例因腹胀并癫痫发作入院。3例均经CT检查证实大脑钙化结节灶。

Logistic regression analysis discovered that the symptoms correlate with outcome are consciousness,flushed cheeks,anisocoria,accelerated breathing,gruff breathing,fever,spontaneous perspiration,dry stool and foul breath.3 Electroencephalography monitoring22 patients were included and no patient\'s electroencephalogram was normal. Special types of electroencephalogram include 1 spindle coma and 3α,θcoma, and 1 patient\'s electroencephalogram appeared spike and slow wave complex.

Logistic回归分析显示神志、两颧潮红、瞳孔不等、呼吸急促、呼吸气粗、发热、自汗、便于、口臭与结局相关。3.3脑电监测结果共纳入患者22例，生存组和死亡组患者无1例正常脑电图表现，特殊类型脑电有1例纺锤波昏迷，3例α、θ昏迷，1例出现棘慢复合波。

Of the 8 cases, 2 cases showed blood Tb-Ab positive, 1 case was positive to antiacid bacillus of sputum, the rest cases with negative TBAb. 3 patients with 2 showed organs infection, 6 patients passed the tissue biopsy diagnose, body temperature of 6 cases fall to normal after 3-7 days of antituberculotic treatment, mild liver dysfunction appeared in 2 patients, hyperuricemia occurred in 7 patients, in addition, 1 patient developed reject reaction. Among all the cases, 7 cases healed, 1 died of pulmonary fungal infection.

其中2例血Tb-Ab，1例腹水结核抗体阳性，1例痰抗酸杆菌阳性，其余结核抗体阴性，3例患者表现为2个脏器感染，6例通过病理组织活检诊断，6例发热者在抗结核治疗3~7d体温降至正常，2例患者在治疗过程中出现轻度肝功能异常，7例出现高尿酸血症，1例出现移植肾排斥反应。8例患者7例痊愈，1例肺结核患者死于肺部真菌感染。6例发热者在抗结核治疗3~7d体温降至正常。

The reasons of iron ions content overproof in grade Ⅱ desalting water system,such as variation water quality,contamination of regenerant , operation adjustment of pretreatment system and switching operation of bed were discussed.

对二级脱盐水系统中铁离子含量超标的原因，如来水水质发生波动、再生剂受到污染、预处理系统操作调整、床体运行切换等进行了论述。

You were hired to drum up new business, so go and do it.

公司雇你招徕新业务，你就做你的事好了。