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Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证实。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证明。

Method]from may 2000 to november 2007,14 patients(10 females,4 males;mean age 34.6 years)with scapular fractures were treated by open reduction and internal fixation through a posterior approach.eight patients were injurred in traffic accidents,3 due to falling,2 by direct violence and 1 due to cut.there were eleven patients with close fractures and 3 with open fractures.according to hardegger classification system,there were 8 cases of scapular body fracture,9 of scapular spine fracture,6 of scapular acromion,10 of scapular neck fracture,6 of glenoid rim fracture and 4 of glenoid fossa fracture.

车祸伤8例,高处坠落伤3例,直接暴力伤2例,刀砍伤1例。闭合性骨折11例,开放性骨折3例。根据hardegger的分型方法,体部骨折8处,肩胛冈骨折9处,肩峰骨折6处,肩胛颈骨折10处,盂缘骨折6处,盂窝骨折4处。其中混合型骨折10例,合并全身多发伤5例;伴有腋神经损伤1例,肩胛上神经损伤2例。通过后入路手术,对不同类型的肩胛骨骨折使用重建钢板或拉力螺钉和钢丝等固定。[结果]14例患者获得随访,随访时间6个月~3年,平均18个月。

A frameshift mutation at base position 217 of exon 1 and a T/C missense mutation at base position 59 of exon 3 were found in Yingjing angora rabbit with a high frequency; a T/C same-sense mutation at base position 3 of exon 3 was found with similar frequency in three rabbit breeds.

在外显子1的217位检测到由TCT三碱基插入引起的移码突变,在外显子3的59位和3位分别发生了错义突变由T→C和同义突变由T→C。通过计算发现各位点不同的基因型和等位基因频率在3个兔品种中存在较大的差异,位点A、B在长毛兔和肉兔中均有各自的优势基因型和等位基因。

During three successive austral summers from Dec. to March of 1992/1993,1993/1994 and 1994/1995,investigation was carried out on phytoplanktonic chlorophyll a content in Great Wall Bay,Antarctica.The results showed that among the three summers the concentration of chlorophyll a in the summer of 1992/1993 was the highest with an average value of 3.79 mg/m 3 ,the range of chlorophyll a variation in this year was the largest,too.

1992年12月~1993年3月、1993年12月~1994年2月、1994年12月/1995年3月连续3个夏季期间在南极长城湾进行了海洋生态系统调查,利用获得的浮游植物现存量资料进行分析研究发现:1992/1993年夏季叶绿素a含量最高,平均值为3.79mg/m3,变化幅度也最大,为10.10mg/m3。

3The modified repoxy resin according to the above item(1) or (2),wherein X in the formula (1) is a hydrocarbon group having 8 to 14 carbon atoms,(4)the modified epoxy resin according to any of the above items (1) to (3),wherein the compound of the formula (1) is 1,1-bis-(4-hydroxyphenyl)-3.3.5-trimethyl-cyclohexane.

三修改repoxy树脂按上述第项或第(2),其中X在公式(1)是烃77 有8至14个碳原子,(4)环氧树脂据上述任何第项至第(3),其中复合配方(1) 1,1 双(4-羟基苯)-3.3.5-三甲基cy clohexane。陈丰应该没有错了

The result shows that optimized formula: triazophos is 30%; butyl alcohol is 1%~3%; xylene is 3%~5%; DMF is 3%~5%; emulsifier 500(superscript #, NPEPO4, 1602(superscript #) and 400(superscript #) is 17%~20% and water else.

结果表明,优化的配方为:三唑磷30%,丁醇1%~3%,二甲苯3%~5%,二甲基甲酸胺3%~5%,乳化剂(500、NPE-PO4、1602和400按一定比例混合)17%~20%,水余量。

A unit of astronomical length based on the distance from Earth at which stellar parallax is one second of arc and equal to 3.258 light-years, 3.08'×03 kilometers, or .98 × 0 3 miles.

秒差距:天文学自地球起的距离单位,星视差为角秒,等于3.258光年,3.08'×03公里,或。98×0 3英里

The effects of solvent type,concentration of the aqueous ammonia on the yields of target compounds were studied. The different results between 2,6-diamino-3,5-dinitropyridine (and 2,6-diamino-3,5-dinitropyridine-1-oxide) and 3-nitropyridien were analyzed.

探讨了溶剂类型、氨水浓度等反应条件对目标化合物产率影响,并讨论了2,6-二氨基-3,5-二硝基吡啶(2,6-二氨基-3,5-二硝基吡啶-1-氧化物)氧化胺化反应结果与3-硝基吡啶不同的原因。

The optimal medium components were: sodium alginate 3 g/L,(NH4)2SO4 3 g/L, NaCl 20 g/L, KH2PO4 0.1 g/L, CaCl2 0.1 g/L; The optimal culture conditions were: 25 ml medium in 250 mL Erlenmeyer flask, inoculum's volume 3%, shaking speed of 150 r/min, initial pH 7.5, at 28°C for 24 h.

该菌株产酶的最佳培养基组成为:褐藻胶3 g/L、(NH4)2SO4 3 g/L、NaCl 20 g/L、KH2PO4 0.1 g/L、CaCl2 0.1 g/L;最佳培养条件为:250 mL三角烧瓶中装液量25 mL、接种量3%、摇瓶转速150 r/min、pH7.5、培养温度为28℃、培养时间为24 h。

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What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢?

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我,一个心中布满虚荣的女孩子真实的想法。

Oh, and I bought myself a new laptop," he said."

哦,我还给我自己买了一个新笔记本"他说。"