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Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证实。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证明。

Method]from may 2000 to november 2007,14 patients(10 females,4 males;mean age 34.6 years)with scapular fractures were treated by open reduction and internal fixation through a posterior approach.eight patients were injurred in traffic accidents,3 due to falling,2 by direct violence and 1 due to cut.there were eleven patients with close fractures and 3 with open fractures.according to hardegger classification system,there were 8 cases of scapular body fracture,9 of scapular spine fracture,6 of scapular acromion,10 of scapular neck fracture,6 of glenoid rim fracture and 4 of glenoid fossa fracture.

车祸伤8例,高处坠落伤3例,直接暴力伤2例,刀砍伤1例。闭合性骨折11例,开放性骨折3例。根据hardegger的分型方法,体部骨折8处,肩胛冈骨折9处,肩峰骨折6处,肩胛颈骨折10处,盂缘骨折6处,盂窝骨折4处。其中混合型骨折10例,合并全身多发伤5例;伴有腋神经损伤1例,肩胛上神经损伤2例。通过后入路手术,对不同类型的肩胛骨骨折使用重建钢板或拉力螺钉和钢丝等固定。[结果]14例患者获得随访,随访时间6个月~3年,平均18个月。

A frameshift mutation at base position 217 of exon 1 and a T/C missense mutation at base position 59 of exon 3 were found in Yingjing angora rabbit with a high frequency; a T/C same-sense mutation at base position 3 of exon 3 was found with similar frequency in three rabbit breeds.

在外显子1的217位检测到由TCT三碱基插入引起的移码突变,在外显子3的59位和3位分别发生了错义突变由T→C和同义突变由T→C。通过计算发现各位点不同的基因型和等位基因频率在3个兔品种中存在较大的差异,位点A、B在长毛兔和肉兔中均有各自的优势基因型和等位基因。

During three successive austral summers from Dec. to March of 1992/1993,1993/1994 and 1994/1995,investigation was carried out on phytoplanktonic chlorophyll a content in Great Wall Bay,Antarctica.The results showed that among the three summers the concentration of chlorophyll a in the summer of 1992/1993 was the highest with an average value of 3.79 mg/m 3 ,the range of chlorophyll a variation in this year was the largest,too.

1992年12月~1993年3月、1993年12月~1994年2月、1994年12月/1995年3月连续3个夏季期间在南极长城湾进行了海洋生态系统调查,利用获得的浮游植物现存量资料进行分析研究发现:1992/1993年夏季叶绿素a含量最高,平均值为3.79mg/m3,变化幅度也最大,为10.10mg/m3。

3The modified repoxy resin according to the above item(1) or (2),wherein X in the formula (1) is a hydrocarbon group having 8 to 14 carbon atoms,(4)the modified epoxy resin according to any of the above items (1) to (3),wherein the compound of the formula (1) is 1,1-bis-(4-hydroxyphenyl)-3.3.5-trimethyl-cyclohexane.

三修改repoxy树脂按上述第项或第(2),其中X在公式(1)是烃77 有8至14个碳原子,(4)环氧树脂据上述任何第项至第(3),其中复合配方(1) 1,1 双(4-羟基苯)-3.3.5-三甲基cy clohexane。陈丰应该没有错了

The result shows that optimized formula: triazophos is 30%; butyl alcohol is 1%~3%; xylene is 3%~5%; DMF is 3%~5%; emulsifier 500(superscript #, NPEPO4, 1602(superscript #) and 400(superscript #) is 17%~20% and water else.

结果表明,优化的配方为:三唑磷30%,丁醇1%~3%,二甲苯3%~5%,二甲基甲酸胺3%~5%,乳化剂(500、NPE-PO4、1602和400按一定比例混合)17%~20%,水余量。

A unit of astronomical length based on the distance from Earth at which stellar parallax is one second of arc and equal to 3.258 light-years, 3.08'×03 kilometers, or .98 × 0 3 miles.

秒差距:天文学自地球起的距离单位,星视差为角秒,等于3.258光年,3.08'×03公里,或。98×0 3英里

The effects of solvent type,concentration of the aqueous ammonia on the yields of target compounds were studied. The different results between 2,6-diamino-3,5-dinitropyridine (and 2,6-diamino-3,5-dinitropyridine-1-oxide) and 3-nitropyridien were analyzed.

探讨了溶剂类型、氨水浓度等反应条件对目标化合物产率影响,并讨论了2,6-二氨基-3,5-二硝基吡啶(2,6-二氨基-3,5-二硝基吡啶-1-氧化物)氧化胺化反应结果与3-硝基吡啶不同的原因。

The optimal medium components were: sodium alginate 3 g/L,(NH4)2SO4 3 g/L, NaCl 20 g/L, KH2PO4 0.1 g/L, CaCl2 0.1 g/L; The optimal culture conditions were: 25 ml medium in 250 mL Erlenmeyer flask, inoculum's volume 3%, shaking speed of 150 r/min, initial pH 7.5, at 28°C for 24 h.

该菌株产酶的最佳培养基组成为:褐藻胶3 g/L、(NH4)2SO4 3 g/L、NaCl 20 g/L、KH2PO4 0.1 g/L、CaCl2 0.1 g/L;最佳培养条件为:250 mL三角烧瓶中装液量25 mL、接种量3%、摇瓶转速150 r/min、pH7.5、培养温度为28℃、培养时间为24 h。

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推荐网络例句

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。