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The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.

1、健康雌性SD大鼠18只,随机分为电穿孔组和非电穿孔组,每组9只,制作右下肢趾长伸肌失神经支配模型;EP组为将质粒pEGFP-N1溶液50μl(0.8μg/μl)注射入右趾长伸肌后,立即于两侧腱腹交接处给予电穿孔,电穿孔参数为:电场强度为200V/Cm,脉冲100μs,频率1Hz,施加10次脉冲;NEP组仅质粒pEGFP-N1溶液注射;转染后1、2、3周,荧光显微镜下观察趾长伸肌中GFP的表达情况,转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况,检测和优化体内转染效率。2、健康雌性SD大鼠78只,随机分为失神经对照组、RC2基因治疗组(RC2组),MAFbx基因治疗组,每组24只,制作右下肢趾长伸肌失神经支配模型,余6只为正常组;分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌,之后给予电穿孔,方法同上;治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化,治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积,肌细胞超微结构变化以及肌纤维中蛋白含量变化。

Companion of accentuation of the illness 2 years ago is oedema of discontinuous double lower limbs, nightly clonic breath is difficult, colour of electrocardiogram, heart exceeds an examination to diagnose for " coronary heart disease, heart expands, atrium is vibratile, hypertensive disease 3 class ", the illness after outer courtyard gives step-down, strong heart treatment improves, grant for a long time to block Tuopuli 25mg is daily 3, alvine dissolve aspirin 75mg is daily 1, ester 10mg of Shan Li of nitric acid different is daily take 3 times, the illness is stable.

因活动后心慌、气促10年,伴反复抽搐,伴短暂意识丧失11个月,复发1天入院。10年前出现体力活动后心慌、气促,在当地医院诊断为"冠心病",治疗情况不详。2年前病情加重伴间断双下肢水肿、夜间阵发性呼吸困难,心电图、心脏彩超检查诊断为"冠心病、心脏扩大、心房颤抖,高血压病3级",在外院给予降压、强心治疗后病情好转,长期予卡托普利25mg每日3次、肠溶阿司匹林75mg每日1次、硝酸异山梨酯10mg每日3次服用,病情稳定。

Originally eat 150ML / day five times, from 3 months after I go to work, feeding on a problem, to the present or each 150ML, 4 months after the beginning of Garmisch powder, half spoon of rice cereal to tune, out to 3 / 4, also increases over egg yolk, as not interested in hindsight, she always seemed reluctant to eat, they cut into the 4 th day of milk, the volume of the same, plus 1 / 3 spoon of rice flour in milk, the still do not have to闹着eating, tongue is not white, the other what is good and can play love to laugh, to sleep or every two hours during the day we should sleep 23:30 after eating milk can sleep the next morning, 8:30, still do not seem hungry.

原先能吃150ML/每日五次,从3个月我上班后,吃奶就成问题,到现在还是每次150ML,4个月时开始加米粉,半调羹米粉调的米糊能,吐出去3/4,也加过蛋黄,一样没兴趣,后来看她吃饭总是显得不情愿,就减成一日4次奶,量不变,加1/3调羹米粉在奶里,还是不闹着要吃,舌苔不白,其他什么都很好,能玩爱笑,睡觉也好,白天隔2小时就要睡一觉,晚上11点30分吃完奶能睡到第二天早上8点30分,还是不显得饿。

From June 30 this year began, the market interest rate has fallen by 70 basis points more than 1 year, 3-year and 7-year bond yield to maturity showed a clear trend towards decline, the current 1,3-year treasury bonds yield less than 3%.

从今年6月30日开始,市场利率70个基点,跌幅超过1年,3年期和7年期国债的到期收益率呈明显下降趋势走向,目前的1,3年期国债收益率低于3%。

The acute toxicities of 2-chloro-4-nitroaniline, 4-chloro-3-nitroaniline, 2-chloro-5-nitroaniline, 2, 4-dichloroaniline, 3, 4-dichloroaniline, 2, 5-dichloroaniline, 2, 3-dichloroaniline to zebra fish were conducted in lab. 48h LC〓 values were 11. 18, 4. 62, 9. 23, 9. 55, 7. 07, 6. 68, 0.62mg/L respectively, and 96h LC〓 values were 6. 99, 2. 58, 8. 63, 7. 79, 6. 08, 5. 23, 0.49mg/L respectively.

1研究了2-氯-4-硝基苯胺、4-氯-3-硝基苯胺、2-氯-5-硝基苯胺、2,4-二氯苯胺、3,4-二氯苯胺、2,5-二氯苯胺、2,3-二氯苯胺对斑马鱼的急性毒性,经计算48h LC〓分别为11.18、4.62、9.23、9.55、7.07、6.68、0.62mg/L,96h LC〓分别为6.99、2.58、8.63、7.79、6.08、5.23、0.49mg/L,这7种化合物均属于高毒物质。

Under the optimum conditions, the linear range of the method was 0-80 μg/L. The detection limit was 4.58×10^(-7) g /L.The catalytic reaction was the zeroth order one. The apparent activation energy and the reaction rate constant was found to be 96.04 kJ/mol and 3.61×10^(-3)/s, respectively. The sensitivity was increased by 3 times in the presence of surfactant.

在最佳实验条件下,方法线性范围为0~80μg/L,检出限为4.58×10^(-7)g/L;催化反应为动力学零级反应,表观活化能为96.04kJ/mol,反应速率常数为3.61×10^(-3)/s;反应在表面活性剂存在下,灵敏度提高3倍。

Efficacious drugs were made of these species for treatment of cough, phlegm, breathe heavily, especially, to cure lung disease. We have investigated on the Chemical constituents of Adenophora stenanthina subspecies xifengsis collected from Gansu Qingyang, and twenty four compounds were isolated from ethanol extraction by chromatography technigues. Twenty compounds were identified by means of chemical and spectra methods(IR, FAB-MS, EI-MS,〓,〓, DEPT , among which three compounds were new as 3βvanilloyloxy-stigmast-5-ene-7β-one; 3β-Vanilloyloxy-stigmast-5-en-7 β-ol; 3β-vanilloyloxy-stigmast-5-en-7β, 15 〓-diol.

我们对采自甘肃庆阳的西峰沙参Adenophorastenanthina subsp.xifengensis)进行了化学成分的研究,利用现代层析手段从该植物的乙醇浸膏中分离出二十四个化合物,经化学方法和波谱技术(红外,质谱,核磁共振谱)鉴定了二十个化合物的结构,其中三个为新的化合物,它们分别是:豆甾-5-烯-7-酮-3β-香草酸酯;7β-羟基-豆甾-5-烯-3β-香草酸酯;7β,15ξ-二羟基-豆甾-5-烯-3β-香草酸酯。

Plus 2-3 kg of material that can burn 2-3 hours, 1-3 minutes aerogenes.

加2—3公斤料,能燃烧2—3个小时,1至3分钟产气。

Results:Normal segment:DEM 8.89±1.21mm,VDEM2.30±0.76cm/s;Hypokinetic segment:DEM 4.56±0.53mm,VDEM 1.89±0.35cm/s;Akinetic segment:DEM 2.13±0.45mm,VDEM 0.95±0.07cm/s.There was a significant overall difference in DEM and VDEM (P0.0001)among wall motion grades.1/3 VDEM was greater than 2/3 VDEM in normal(P0.0001)and hypokinetic(P0.05)segments,though 1/3 VDEM was slower than 2/3 VDEM in akinetic segments(P0.0001).There was also high correlation between 1/3 DEM and 1/3DEMF in both normal(r=0.81,P0.0001,y=0.75x+0.16)and OMI(r=0.91,P0.0001,y=0.74x+0.10)groups.Conclusion:Analysis of color kinesis image can quantitatively assess regional and global diastolic function.

结果:运动正常节段:DEM8.89±1.21mm,VDEM2.30±0.76cm/s;运动减弱节段:DEM 4.56±0.53mm,VDEM1.89±0.35cm/s;运动消失节段:DEM2.13±0.45mm,VDEM0.95±0.07cm/s;三种不同室壁运动状态间的DEM、VDEM均存在显著性差异PDEM显著快于2/3VDEM(4.56±1.18cm/s vs 1.26±0.34cm/s,PDEM略快于2/3VDEM2.14±0.66cm/s vs 1.76±0.47cm/s,PDEM显著低于2/3 VDEM(1.23±0.52cm/s vs 1.37±0.30cm/s,P结论:彩色室壁运动技术能定量评价左室局部和整体舒张功能。

Results Compound albicanyl caffeate displayed strong antifungal property with MIC 4 μg/ml, while moderate antifungal effects (MIC16 μg/ml)were observed with compounds emodin, resveratrol, 3,4'-difluorostilbene, 2,2'-difluorostilbene and 2,3',5'-trifluorostilbene. Pakyonol, catechin, ampelopsin A, 3,4,4'-trihydroxyl- bibenzyl, 2,4'-difluorostilbene, 4-fluorostilbene,2-methyloxy-4'-fluorostilbene showed lower activity with MIC 32 μ g/ml.

结果化合物albicanyl caffeate对白色念珠菌表现出强的抗真菌活性,MIC为4μg/ml;而化合物大黄素、白藜芦醇、3,4'-二氟二苯乙烯、2,2'-二氟二苯乙烯及2,3',5'-三氟二苯乙烯显示出中等程度的抗真菌活性,MIC为16μg/ml;双联苄pakyonol、多酚类儿茶素、白藜芦醇二聚体野葡萄素A、3,4,4'-三羟基联苄、2,4'-二氟二苯乙烯、4-氟二苯乙烯及2-甲氧基-4'-氟二苯乙烯显示出较弱的抗真菌活性,MIC为32 μg/ml。

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推荐网络例句

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢?

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我,一个心中布满虚荣的女孩子真实的想法。

Oh, and I bought myself a new laptop," he said."

哦,我还给我自己买了一个新笔记本"他说。"