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Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分为4个部分,第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究,确立了一种较好的培养方法:与颗粒细胞共培养,找到了一种适合猪卵母细胞体外成熟的培养基:mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸,成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%,国外报道的最高成熟率为(85.7±4.1)%;第二部分对猪卵母细胞孤雌激活的化学方法进行了研究,确立了化学激活猪卵母细胞的两种最佳方法:1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min,再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中,卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min,再与8mM的DTT共孵育30min,卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%;第三部分对孤雌激活胚胎的培养条件进行了研究,确立了一种最佳的胚胎培养条件:在SOF简单培养基中添加颗粒细胞进行前3天的培养,然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养,其桑椹胚和囊胚的发育率为(59.5±3.2)%;第四部分研究了IGF-I

All those that are justified, God vouchsafes, in and for his only Son Jesus Christ, to make partakers of the grace of adoption: Eph 1:5 Ga 4:4,5 by which they are taken into the number, and enjoy the liberties and privileges of the children of God; Ro 8:17 Joh 1:12 have his name put upon them, Jer 14:9 2Co 6:18 Re 3:12 receive the Spirit of adoption, Ro 8:15 have access to the throne of grace with boldness; Eph 3:12 Ro 5:2 are enabled to cry, Abba, Father; Ga 4:6 are pitied, Ps 103:13 protected, Pr 14:26 provided for, Mt 6:30,32 1Pe 5:7 and chastened by him as a father; Heb 12:6 yet never cast off, La 3:31 but sealed to the day of redemption, Eph 4:30 and inherit the promises, Heb 6:12 as heirs of everlasting salvation.

1Pe 1:3,4 Heb 1:14 1 )所有那些是合理的,上帝vouchsafes ,在和他的独生子耶稣基督,使partakers的恩典通过:弗1:5加文4:4,5其中,他们是考虑到该号码,并享受自由和特权的孩子们的上帝;滚装8时17 joh 1:12有他的名字,把他们的,张哲14时09 2co 6:18重新3时12分收到的精神,通过反渗透8:15进入王位的恩典与气魄;厄3时12滚装5时02分都可以哭,天父,创始者;加文四比六,是怜悯,聚苯乙烯103:13保护, 14时26分公关提供的,吨6:30,32 1pe 5时07分和chastened由他作为一个父亲;希伯来书12时06分,但从来没有投过,香格里拉3时31分,但密封,以当天的赎回,弗4:30和继承的诺言,以弗所书6时12分,作为继承人的永恒救赎。

Germany- made plasma cutter and welder (line welding machine and circular welding machine, one set for each); 12 argon welding machines; 4 electric welding machines; 1 spot welder; 3 CO2 welders; 2 oil buffer machines; 2 motor rolling machines; 8 motor wheels; 6 overhead travelling cranes; 3 pressure testers; water- pressured machine for making tank dish cover; welding beads stress reliever; computers for business and graphic design 3 sets for each; stainless steel tube automatic welder and steel tube cutter each for 2 sets.

德制自动电离子切割焊接机组(直线焊接及圆周焊接机各一套)氩焊机12 台、电焊机4 台、点焊机1 台、CO2焊机3 台、油压机床2 台、电动卷圆机2 台、电动滚轮8 台、电动天车6 台、钻床3 台、试压机3 台、卫生桶制造专用水压成型设备壹套、焊道应力消除机1 台、业务用电脑及图面设计用电脑各3 套、不袗管自动焊接机及钢片切管机各2 套。

We are interesting in the synthesis and the application of 1,2,3-triazoles inthis paper, which including the following:In the first chapter, we reviewed mainly the development of Huisgen\'s 1,3-dipolar[3+2]-cycloaddition from organoazides and alkynes or alkenes to synthize1,2,3-triazoles in recent years.

本论文针对1,2,3-三唑的合成和应用,主要包括以下方面:第一章对近几年来1,2,3-三唑合成研究进行了概述,总结了有机叠氮和炔烃、烯烃的1,3-偶极环加成反应以及其他方法合成三唑的进展。

The average recoveries of captan and folpet from the apples spiked at three levels ranged from 69.3%-106% and 101%-108%, with RSD of 3.7%-4.7% and 1.3%-5.4%, respectively.

苹果样品中3个添加水平的平均加标回收率为克菌丹69.3%~106%,RSD为3.7%~4.7%;灭菌丹101%~108%,RSD为1.3%~5.4%。

Result: After the operation, the necrosis of skin flap 2 examples(4.3%), the cutaneous dropsy 6 examples(13.0%), the lymph edemas of upper limb 4 examples(8.7%), the contracture of the pectoral muscle and the same upper arm's movement disturbance 2 examples(4.3%), 3 years survival rate 83.9%, 5 years survival rate 64.5%,Ⅰ issue 81.1%,Ⅱ issue 52.3%,Ⅲissue 32.2%.

结果:术后发生皮缘坏死2例(4.3%),皮下积液6例(13.0%),上肢淋巴性水肿4例(8.7%),胸肌挛缩伴同侧上臂运动障碍2例(4.3%)。3年生存率83。 9%; 5年生存率64。 5%,其中Ⅰ期为81。

Other possible cleaning fluids that have been suggested include:- vinegar, rubbing alcohol, and water- 1/3 white vinegar, 1/3 rubbing alcohol, 1/3 water, and 3 drops dishwashing liquid (1 quart total)- Swiffer WetJet wood floor cleaner- WindexHere are some tips on spot cleaning:.

曾经包括:醋、擦酒精、水-1/3白醋1/3擦酒精,1/3水、三滴洗净剂(1石英共)-木地板清洁swifferwetjet-windexhere一些小费现场清理:。

RESULTS: In Phaco group, 79.2% of the patients visual acuities at the first day after operation were≥0.3. In this group, the nuclear hardnesses of some patients catract were ≤Ⅲ, and others ≥Ⅳ; complications occurred in the former including capsule rupture and vitreous prolapse(4.3%), corneal edema (22.2%); while the latter were 13.7% and 68.6%, respectively.

结果:Phaco组视力≥0.3者79.2%,核硬度≤Ⅲ者后囊膜破裂玻璃体脱出发生率4.3%,角膜水肿发生率22.2%,核硬度≥Ⅳ者后囊膜破裂玻璃体脱出13.7%,角膜水肿68.6%;MSICS组视力≥0.3者78.1%,核硬度≤Ⅲ者后囊膜破裂玻璃体脱出发生率3.8%,角膜水肿发生率19.0%,核硬度≥Ⅳ度者后囊膜破裂玻璃体脱出发生率5.7%,角膜水肿发生率14.3%。

Among the special nine pollutants, however, eight of them, including Hexachlorobenzene, N-Nitrosodi-N-propylamine, 3,3-dichlorobenzidine, 2,4,6-trichlorophenol, 2,3,4,5,6-pentachlorophenol, indeno (1,2,3-cd) pyrene, 4,4'-DDT and Heptachlor were in need of concern while another chemical: dibenzanthracene be of special concern. The sources of these chemicals were discussed and some control strategies were suggested based on the risk assessment.

结果表明,有18种有机污染物超过了USEPA的标准浓度要求,其中9种物质具有一定的生态风险,其中六氯苯、N-亚硝基二正丙胺、3,3'-二氯联苯胺、2,4,6-三氯酚、五氯酚、茚并(1,2,3-cd)芘、4,4'-滴滴涕、七氯在内的8种化合物具有一定的风险,而多环芳烃二苯并蒽需要引起更高警惕;在此基础上探讨了这些化合物的来源及作用,并提出了相应的防治建议。

Result 5 compounds in Hickory nut oil were identified, and they were linoleic acid, oleic acid, linolenic acid, palmitic acid and stearic acid, and their contents were 63.48%, 19.90%, 11.96%, 3.31% and 1.35% resp. The fatty acid ratio of n-6 to n-3 was 5.31, which was close to that of recommended by China (4-5):1. The ratio of saturated fatty acid, monounsaturated fatty acidand polyunsaturated fatty acids in Hickory nut oil was 1:4.3:16, the content of polyunsaturated fatty acids was slightly higher, but it mainly contained linoleic acid and a-linoleic acid, which were inevitable fatty acid to human body.

结果]共鉴定出山核桃仁油中的5中化合物,即亚油酸、油酸、亚麻酸、棕榈酸和硬脂酸,其含量分别为63.48%、19.90%、11.96%、3.31%和1.35%;山核桃仁油中n-6: n-3脂肪酸比例为5.31,与我国推荐的n-6:n-3脂肪酸比例(4~5):1非常接近;山核桃仁油中饱和脂肪酸:单不饱和脂肪酸:多不饱和脂肪酸为1:4.3:16,多不饱和脂肪酸含量偏高,但多不饱和脂肪酸主要包括亚油酸和a-亚麻酸,均为人体必须脂肪酸。

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推荐网络例句

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢?

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我,一个心中布满虚荣的女孩子真实的想法。

Oh, and I bought myself a new laptop," he said."

哦,我还给我自己买了一个新笔记本"他说。"