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After the RT-PCR detection of the total RNA from heart tissue, we found that the CD59 gene and MCP gene were expressed. 4. The CD59 gene and MCP gene were mixed with liposome, then used to produce transgenic swine by 3 methods. Firstly, the gene/ liposome complex was directly injected into male swine's testis. The transfected males copulated with 8 females each 44 days later.

其中睾丸注射法处理公猪1头,44d后配母猪8头,7头受孕,产仔73头,结果检测到PCR阳性猪3头(阳性率4.3%);输精管注射法处理公猪3头,8d后配母猪3头,0头受孕;外源基因/精子共孵育法处理公猪4头,处理母猪4头,1头受孕,产仔11头,结果检测到PCR阳性猪1头(阳性率9%)。

Capsule ovoid, not angular, 4--6 × 3--4.5 mm, not or only slightly contracted distally when dehisced; leaves (2--)3--5(--8) mm wide

蒴果卵球形,并非具角, 4--6 * 3--4.5 毫米,唯一的并非或稍签订在开裂时;叶(2-)3-5(-8)毫米宽 3 ALETRIS stenoloba 狭瓣粉条儿菜

The yield was 47.3%. A series of21 azo-1,3,4-thiadiazole compounds were synthesized by diazotizing of2-amino-5-alkyl-1,3,4-thiadiazole with nitrosylsulfuric acid, then couplingwith phenol, substituted phenol or naphthol. The yields were 19.6~58.8%.16 of them were new compounds.

制得的2-氨基-5-烃基-1,3,4-噻二唑用亚硝酰硫酸进行重氮化,再与苯酚、取代苯酚或萘酚发生偶合反应合成了21个偶氮-1,3,4-噻二唑类化合物,其中16个为新化合物,收率为19.6~58.8%。

Clinical outcome measures were mortality and the modified Rankin scale score, dichotomized into favorable (0 to 2) and nonfavorable (3 to 6), at 3 months.

溶栓治疗3个月后改良Rankin量表的评分为0~2分者为预后良好;溶栓治疗3个月后改良Rankin量表的评分为3~6分者为预后不良。

Methods: 2-ethyl aniline was used as starting material of the synthesis and was processed by acetic anhydride acylation, strong nitric acid nitraction at low temperature, and desacyl synthesis to produce 2-ethyl-5-nitro aniline. The product was then synthesized to 3-methyl-6-nitro-lH-indazole by using ring-closing reaction in the presence of NaNO2, and then ethylated to obtain 2, 3-diethyl-6- nitro-2H-indazole. The latter compound was then reduced by SnC12/Hcl to get target compound 2, 3-diethyl-6-nitro-2H-indazole.

以2-乙基苯胺为原料经醋酐酰化、浓硝酸低温硝化及去酰基保护合成2-乙基-5-硝基苯胺,后用亚硝酸钠关环得3-甲基-6-硝基-1H-吲唑,经硫酸二甲酯甲基化得2,3-二甲基-6-硝基-2H-吲唑,最后用氯化亚锡将其还原得到目标产物,通过1HNMR确定结构与目标产物一致。

Followed up for 1 year to 3 years,5 cases recurred in 30 cases.3 cases were excised again,and 2 cases were excised for three times,and the results were satisfactory.

获随访30例(1~3年),5例术后复发,3例行第二次手术,2例先后行3次手术,最终效果良好。

Atteusor fasciae latae,liuking form greater trochanter to tuber is chiaclican,its lower 1/3 and anteri or 1/3 is the most suitable site to inject.

从大转子到坐骨结节连线以上的臀部外上区,其下中1/3和前1/3区的上部最适宜肌肉注射,上、中1/3区下部两横指处可作被选部位,其余区域不宜肌肉注射。

The contact angle of glassfiber epoxy resin fluoridized by 3-(1H, 1H, 5H-Octafluoropentyloxy)-1, 2-Propenoxide hasincreased from 51.3°to 75.3°, and the surface tension has reduced from 36.5mN/m to25.5mN/m accordingly.

同时经过环氧丙基八氟戊醚处理的环氧玻璃钢表面对水的接触角也由原来的51.3°增大到75.3°,其表面张力由36.5mN/m减小到25.5mN/m,达到一定的疏水疏油效果。

So in order to get recombinant NT-3, the rat NT-3cDNA was reconstructed in a mammalian expression vector and transfected into incasing CHO cells by liposome-mediated method. The clones of the cells transferred with rNT-3cDNA were selected by G418. The Chinese hamster ovary cell line stably producing recombinant rat NT-3 was established.

因此,为了获得基因重组NT-3,研究其生物效应,本文成功构建了重组大鼠NT-3基因真核生物细胞稳定表达载体,并利用脂质体介导,转染进CHO细胞;经G418筛选,建立了中国仓鼠卵巢细胞系,稳定表达重组大鼠NT-3。

The surface modification was most successful when the film was immersed in the limos (1,1,1,3,3,3hexamethyl disilazane) and toluene solution at 80°C.

由研究得知以甲苯溶液中的HMDS(1,1,1,3,3,3-hexamethyl disilazane)在80°C下对表面改质效果最佳。

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推荐网络例句

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。