阳离子电泳的

基本解释 (translations)

cataphoretic

与阳离子电泳的相关的网络例句 [注：此内容来源于网络，仅供参考]

Because of different binding constants with surface functional group, there is different mobility with different kinds of divalent cation.

不同二价阳离子因为其与细胞表面官能基键结常数不同，而造成不同的电泳迁移率的变化。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The substance with a molecular weight of 6.28kDa has very strong cation character determined by the electrophoresis of diaphragm wafer of fiber of acetic acid. The substance with antibacteria action obtained from forest frog is made up of alanine,aminoacetic acid,leucine,Isoleucine,Proline,aminoglutaric acid,threonine,Serine,lysine.

纯化林蛙皮肤中的抗菌活性物质经醋酸纤维薄膜电泳，表明其具有较强的阳离子特征；经氨基酸分析，表明林蛙皮肤抗菌活性物质是由丙氨酸、甘氨酸、亮氨酸、异亮氨酸、脯氨酸、中国林蛙皮肤抗菌肽的分离纯化和鉴定一摘要谷氨酸、苏氨酸、丝氨酸、赖氨酸组成。

Between the ages of 20 and 70, one-third of those cells die and are not replaced in men," said Goldspink."

D。胶体微粒具有较大的表面积，能吸附阳离子或阴离子，在电场作用下产生电泳现象

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

The capillary electrophoretic analysis of inorganic ions in rain water and drinking water was studied.

本文建立了不同水样中无机离子的毛细管电泳分离分析方法，考察了其阴离子和阳离子不同的测定条件；测定了雨水中五种阴离子和饮用水中四种阳离子的浓度

Analysis and determination of inorganic anions in rain water by capillary electrophoresis

本文建立了不同水样中无机离子的毛细管电泳分离分析方法，考察了其阴离子和阳离子不同的测定条件；测定了雨水中五种阴离子和饮用水中四种阳离子的浓度。

It was found that the unblocking temperature of the cationic blocked isocyanatc is 117℃, and the practical starting curing temperature of the paint film is about 140℃. The paint film can be cured completely at 180℃ for 30 min.

结果表明：丁醇封闭的阳离子型封闭异氰酸酯解封温度为117℃，电泳漆膜的实际起始固化温度为140℃左右，在180℃、30min可固化完全。

与阳离子电泳的相关的网络解释 [注：此内容来源于网络，仅供参考]

cataphoretic：阳离子电泳的

cataphoresis 阳离子电泳 | cataphoretic 阳离子电泳的 | catcher 捕集器

cataphoretic mobility：阳离子电泳迁移率

cataphoretic migration speed | 阳离子电泳迁移速度 | cataphoretic mobility | 阳离子电泳迁移率 | cataphoretic | 阳离子电泳的

catapetalous：花瓣下的,下联瓣的

catamenia 月经 | catapetalous 花瓣下的,下联瓣的 | cataphoresis 阳离子电泳,电泳