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Results: The expression of both mRNA and protein of c-myc were obvious in the bud and cap stages,while decreased in the bell stage, then increased in ameloblast and dentinoblast when dentin formation. The expressions of c-myc were stronger in mesenchymal cells than in epithelial cells. The expression and distribution of c-myc mRNA is consistent with that of c-myc protein.
结果:c-myc mRNA及蛋白的表达在蕾状和帽状期明显,钟状期减弱,而牙体组织形成期成釉细胞、成牙本质细胞及间充质牙乳头细胞表达又增强。c-myc蛋白表达和分布与c-myc mRNA转录基本一致。
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RESULTS: CaB was not detected during early odontogenesis from the dental lamina to the late c ap stage .Small amount of the protein first appeared in ameloblast and some of dif ferenting cells in the surface of dental papilla in early bell stage. As to late bell stage, the cytoplasm of ameloblasts and odontoblasts with secreted dentin was all stained strongly, as well as some of n uclei.
结果:人牙胚蕾、帽状期成釉器无CaB的表达;钟状早期成釉器的内釉细胞及部分牙乳头表面处于分化状态的细胞弱阳性染色;钟状晚期成釉器的成釉细胞、成牙本质细胞及部分胞核呈现强阳性染色。
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In bud stage, Dlx1 and Dlx2 were expressed in the dental mesenchyme, and in dental epithelium.
蕾状期:Dlx1和Dlx2在成釉器中央细胞及周围外胚间充质细胞为阳性表达。
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enamel bud:釉蕾
珐琅质釉质 enamel | 釉蕾 enamel bud | 釉骨髓 enamel pulp