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Methods The amino modified wild-type and mutant probes of 16 loci ˇ1888, 3200, 3206, 3243, 3290, 3316, 3394, 3593, 3426, 12026, 12258, 14577, 14693, 14709, 16189, 16223ˇ in mitochondrial genome were immobilized onto the activated slides with aldehyde surface using Biochip Arrayer, then they were incubated overnight in a humid chamber at room temperature.
方法将氨基修饰的16个常见易感基因位点ˇ1888ˇ3200ˇ3206ˇ3243ˇ3290ˇ3316ˇ3394ˇ3593ˇ3426ˇ12026ˇ12258ˇ14577，14693ˇ14709ˇ16189ˇ16223ˇ的野生型和突变型探针ˇ通过Packard芯片点样仪点样于醛基修饰的基片上ˇ室温保湿过夜固定。
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Serum free medium containing 0,16,32,64,128/μg/mL was added 24 hours after culture and cultured for another 24 hours. Cells were collected centrifugally and resuspended in 75% alcohol. Finally, cells were fixed 4℃overnight. Then cells were stained with PI for 30 min under 4℃.. FCM was run with parameter FL2 - A.
流式细胞仪检测细胞凋亡率对数生长期的BIU-87细胞接种于培养瓶内，24 h后更换含有终浓度0、16、32、64、12μg/mL的无血清培养液，处理24 h，离心收集，75%冷乙醇悬浮后4℃固定过夜，PI染液4℃避光染色30min，流式细胞仪于FL2-A参数下检测。
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The ammonia was evaporated under decreasing pressure, and the precipitate was filtered off and the liquid was poured into hot saturated picric acid solution to get picrate with a yield of 85.0%. The optimum process starting from imidazole-4,5-dicarboxylic acid was: imidazole-4,5-dicarboxylic acid in acetic anhydride was refluxed to get imidazole-4-carboxylic acid, and the product was esterification in ethanol with a catalyzer of concentrated sulfuric acid to get ethyl imidazole-4-carboxylate, at last the compound above was reduced by LiAlH4 in ether at room temperature to get the target compound, the total yield of this route was 49.6%.
以4，5-二羧酸咪唑为原料的较佳工艺：1 脱羧反应：4，5-二羧酸咪唑40g，醋酐1200ml，回流10h，过滤并将滤液浓缩至干，所得固体加至50%的乙醇溶液回流，过滤并将滤液自然冷却过夜，过滤得固体；2 酯化反应：4(5)-羧酸咪唑50g，乙醇1000ml和浓硫酸60ml，加热回流2h，5%浓度NaOH溶液调节至pH＝8，减压浓缩至干，加入少量水回流，自然冷却过夜并过滤得固体；3 还原反应：LiAlH410g，乙醚300ml和4(5)-羧酸咪唑乙酯28g，常温反应1.5h，小心滴加25ml水后过滤，滤渣溶于300ml甲醇中并过滤，收集所得乙醚和甲醇滤液浓缩至干，将所得固体溶于300ml乙醇中加热回流，后将溶液浓缩至约30ml，冷却并过滤得固体，该路线总收率为49.6%。
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As an added feature, for semipermanent or overnight installations, the valve will also supply water automatically as needed to the tank thereby eliminating manual filling and pumping, and saving wear and tear on the water pump or compressor.
作为一个附加的性能，适用于非永久性或过夜安装，这种阀门还能够根据需要自动将水供应到容器内，因此避免人工注水和接管，减少水泵或压缩机的磨损和划伤。
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In fact, I also received a bouquet of roses, it is in the Valentine's Day I was in a book shop for their own. Over the years, the cold front of the pommel horse every Valentine's Day are rare. I usually fraternizing with those saying that they appreciate the stresses Hupenggouyou impulses to accompany his wife is not really with the situation Guoqingrenjie people, although these often ungrateful guy dawn of the night to talk about the project and aspirations, however, the long run, I seem to have become the most competitive market conditions in women, especially women who have husbands who are husband. become more assured you can be very safe and very frankly working partners and friends, so Most of the Valentine's Day with my husband had seen no boyfriend, no single sex were not celebrate Valentine's, this year's Valentine's Day. extreme fatigue coupled with the continued late into the night because of overtime daily Pok Man. So when I finished the day in the evening after the blog was alone at home fell asleep nausea Cox, a big dream, whoever Cox?
其实，我还收到过一束玫瑰，那是在某年的某个情人节我自己在花店预订给自己的，多年以来，每到情人节都是门前冷落鞍马稀，平时与我称兄道弟的那些口口声声说欣赏讲爱慕的狐朋狗友们不是要陪着老婆就是真的与情人过情人节，虽然这些忘恩负义的家伙们经常天明不过夜地大谈项目和志向，长此以往，我几乎成了在情场上最最没有竞争力的女子，尤其针对那些有夫之妇们的老公们，更加成为大家都可很安全很放心很坦然共事的伙伴和朋友，所以，我的情人节大都与众位没有老公没有男友没有情人的单身美女们普天同庆，今年的情人节，因不断加班熬夜疲惫至极再加上每日博文，所以当我于那天晚上写完当天的博客之后就是独自回家呼呼大睡闷觉，大梦谁先觉？
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Preparation and exosyndrome of liposomal Gd-DTPA 1.1 Preparation small unilamellar vesicles were prepared by combining 0.92 g of distearoylphosphatidylcholinc with 23 mL of distilled watcr.
Gd-DTPA脂质体的制备和表征 1.1制备工艺精密称取一定量二硬脂酰磷脂酰胆碱乙醇胺溶于双蒸水中，置于恒温磁力搅拌器内，在69～72℃恒温条件下超声分散1小时，取一定量Gd-DTPA溶液、蒸馏水和无水乙醇与上述产物混合，获得半透明胶体状物，在室温下过夜，第二天在69～72℃恒温条件下水浴孵化1小时，同时用低流速氮气吹拂液体表面除去有机溶剂无水乙醇。
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The 90th Division reached the Siegfried Line at exactly the spot where the 106th Division had been hit and decimated on December 16. Pvt. Jack Ammons spent the night of February 5-6, 1945, in a bombed-out building on the edge of the line.
第90师抵达齐格菲防线位置恰好是第106师于12月16日遭遇打击和歼灭之处。1945年2月5日到6日夜间，列兵杰克·阿蒙斯在防线边缘一幢炸毁建筑中过夜。
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evaporation：蒸发
(3)蒸发(Evaporation):剩下的热大部分由此途径散失. 如流汗. (4)辐射(Radiation):50%~65%的热由此途径丧失. 3.调整服装的顺序 手套-帽子-夹克的拉及袖口-脱掉一层衣服. 登山住宿装备 帐篷 当你准备于野外过夜可能运用的方式有帐篷,
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shabrack：鞍褥
shabby-genteel 死要面子的 | shabrack 鞍褥 | shack up in 过夜于
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shadberry：美洲蔷薇科扶属植物的果实
shackupin 过夜于 | shadberry 美洲蔷薇科扶属植物的果实 | shadberry 此种植物
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shack up with：同居
shack up in 过夜于 | shack up with 同居 | shack up 同居
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shack up in：过夜于
shabrack 鞍褥 | shack up in 过夜于 | shack up with 同居
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tourists：旅游者
游客(visitor)可分为旅游者(tourists)或者过夜游客(overnight visitors)和当天游客(same-day visitors). 过夜游客的定义是在所造访的地方于私人的或者集体的住所中逗留至少一晚的人. 游客不包括移民搬家、工作者、外交人员、流浪者等.