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The present studies showed that two cell populations were found in haemocytes: large cell with high granularity and small cell with low granularity by flow cytometry FCM on light scanttering pattern. Two distinct cell types were identified based on phase contrast microscope: one type of cell was dark and dioptric aberration, while the other was bright and dioptric strong. By Giemsa and H.E staining, cytoplasmic staining were heterogeneous and internal particles were obvious in one type of cell, while cytoplastic staining were homogeneous and internal particles were inexistent in the other type of cell. By transmission electron microscope, we found that the mitochondria, Golgi apparatus organelles were rich and internal particles were obvious in one type of cells, and contrary to the another cells.
流式细胞术光散射图谱显示血细胞被分两类，一类为颗粒度高的大细胞，另外一类为颗粒度低的小细胞；相差显微镜观察显示，血细胞可分为胞体暗、折光性差和胞体明亮、折光性强的两类； Giemsa和H.E染色显示细胞分为胞质染色不均一、胞内颗粒明显和胞质染色均一、胞内颗粒不明显的两类；透射电镜超薄切片观察显示，颗粒明显的细胞胞质内线粒体、高尔基体等细胞器较丰富，颗粒不明显的细胞胞质内细胞器较少；负染结果表明血细胞主要分为表面不光滑、突起明显和细胞表面光滑、突起较不明显的两类。
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The preparation of specimens with a microtome.
超薄切片机使用术，超薄切片法
- 更多网络解释与超薄切片术相关的网络解释 [注：此内容来源于网络，仅供参考]
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freezing microtomy, cryotomy：冷冻切片术
振动切片机 vibratome | 冷冻切片术 freezing microtomy, cryotomy | 冷冻超薄切片术 cryoultramicrotomy, ultracryotomy
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ultra cryotomy：冰冻超薄切片术
ultra cold storage freezer 超低温冰箱 | ultra cryotomy 冰冻超薄切片术 | ultra deep-freeze equipment 超低温冰箱