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Phaeohyphomycosis is an entity caused by local infection in superficial tissue, skin, cornea, subcutaneous tissue and even systemic infection with a large group of dematiaceous fungi. It is important to promptly diagnose and exactly manage this disorder, otherwise, fatal disseminated phaeohyphomycosis may occur.
暗色丝孢霉病是由一大组条件致病性暗色真菌引起的浅表组织、皮肤、角膜、皮下组织甚至系统性感染，及时、正确的诊治非常重要，否则可导致致命的播散性暗色丝孢霉病。
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MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.
第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。
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For patients with high-risk mycotic corneal ulcer, the conservative therapy was uneffective, but PKP is an effective approach for preservation of eyeballs and restoration of visual function.
对于保守治疗无效的高危真菌性角膜溃疡患者，穿透性角膜移植是挽救眼球和视力的有效方法。关键词：角膜移植；角膜溃疡；真菌病
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indirect ophthalmoscopy：间接检眼镜
角膜缘limbus | 间接检眼镜indirect ophthalmoscopy | 角膜真菌病keratomycosis
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keratomycosis：角膜真菌病
真菌性角膜炎简介:真菌性角膜炎(fungal keratitismycotic keratitis)或角膜真菌病(keratomycosis),临床上较难诊断,容易误诊常因治疗不当而造成失明. 真菌性角膜炎并非少见. 用抗生素治疗无效的所谓"匐行性角膜溃疡"病例中有很大一部分可能就是真菌感染引起.
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media opacities：间质混浊
角膜真菌病 keratomycosis | 间质混浊 media opacities | 接触镜(隐形眼镜) contact lenses