观察仪

基本解释 (translations)

visualist

与观察仪相关的网络例句 [注：此内容来源于网络，仅供参考]

The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

对软骨细胞退变老化进行观察，采用相差显微镜观察其生长情况，透射电镜观察细胞结构，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，免疫细胞化学法和RT-PCR方法检测Ⅱ型胶原鉴定软骨细胞，以MTT比色法描绘生长曲线、检测生长状态，组化法检测老化相关β-半乳糖苷酶，流式细胞仪分析细胞周期和增殖指数。3。

With Stratoscope II, the overall height from the telescope to the top of the launch balloon is 666

在同温层观察仪2号上，望远镜到发射气球顶部的总高度为660英尺，气球总重量超过2吨，另外还带有2吨压舱物，以便于夜间保持高度时抛掉。

"With Stratoscope II, the overall height from the telescope to the top of the launch balloon is 666 feet, the balloon s together weigh over two tons, and another two tons of ballast are carried for later release if height has be maintained during the night."

在同温层观察仪2号上，望远镜到发射气球顶部的总高度为660英尺，气球总重量超过2吨，另外还带有2吨压舱物，以便于夜间保持高度时抛掉。

"With Stratoscope II, the overall height from the telescope to the top of the launch balloon is 666 feet, the balloons together weigh over two tons, and another two tons of ballast are carried for later release if height has be maintained during the night."

在同温层观察仪2号上，望远镜到发射气球顶部的总高度为660英尺，气球总重量超过2吨，另外还带有2吨压舱物，以便于夜间保持高度时抛掉。

The SMMC-7721 hepatoma cell line of experimental groups and control groups were cultured with the addition of sodium selenite, potassium iodide or triiodothyronine (T3). The cell quantity was checked by the MTT assay , and the cell cycle was observed by flow cytometry and levels of AFP and ALB in the culture solution were determined by RIA.The SD rats were divided into positive control group, negative control group, T3 group and mixture group (the mixture of sodium selenite, KI and T3). After two weeks of the administration of sodium selenite、 KI and T3,Walker256 cells were transplanted into the abdominal cavity. The administration was kept on for another two weeks, then observed the morbility and mortality of the rats with ascites carcinoma. At the end, the ascites were for the cells counting and the cell cycles analysising.

方 法：在体外，应用细胞培养的方法，培养SMMC－7721肝癌细胞系，分为试验组和对照组，试验组加入不同浓度Se、I或T3干预，培养一段时间后，通过噻唑蓝试验、流式细胞仪，观察细胞数量及细胞周期的变化，测定细胞培养上清液中甲胎蛋白、白蛋白的水平，观察细胞分化情况；在体内，将试验用SD大鼠分成阴性对照组、阳性对照组、T3组和混合组（为Se、I和T3混合物），首先预防性用药2周，然后给试验组大鼠和阳性对照组大鼠腹腔注射Walker256细胞，制造腹水癌大鼠模型；继续用药2周，观察各组大鼠发病率及死亡率，测量不同时期大鼠血清FT3含量，通过流式细胞仪测量大鼠腹水细胞数及细胞周期变化。

Methods: 5 cases of craniocerebral diseases with secondary hydrocephalus were selected to be monitored by LNP-Ⅰ translumbar epidural space intracranial pressure monitor and LY-Ⅱ ventricular pressure monitor grabually. With horizontal position, we recorded with IVP and ISEDP pressure one time per 5 minutes , The datums of LNP-I monitor in the repeated operations were compared with that of IVP, While variations of clinic symptoms, wave forms of pressure, and intermittent ventriculus dranage in the intracranial hypertension were observed.

选择5例颅脑疾病继发脑积水患者，采用LNP-Ⅰ型经腰硬脊膜外腔颅内压监护仪和LY-Ⅱ型脑室内压监护仪进行持续监测，病人取水平侧卧体位，以每隔5分钟记录IVP和ISEDP压力数据一次，期间多次重复操作LNP-Ⅰ型监护仪与IVP进行对照，同时观察临床症状，压力波形及颅高压时间断行脑室引流时二者变化。

As the DDP group A549DDP cells were treated with IC10 of cisplatin for 48 hours. As the DDP＋Rh2 group A549DDP cells were treated with IC10 of cisplatin and IC5 of gensenoside Rh2 for 48 hours. As the controlling group A549DDP cells were not treated with any kinds of drugs. Changes in cellular morphology were observed by fluorescence microscopy. Peak value apoptosis of A549DDP cells were inspected by flow cytometry. State of PTP was evaluated by ultraviolate spectrofluorometer. Changs of calcium in cells and membrane potential of mitochondrion were determined by flow cytometry. Release of cyt-c from mitochondrion with westton blot Radiative activity of〓Tc-MIBI ingested by cells was measured withγcounter. Results: IC50 of cisplatine to A549 cells was 24uM.

以无毒浓度的Rh2单独作用于A549DDP细胞作为Rh2组，以低效浓度的顺铂单独作用于A549DDP细胞作为DDP组，以无毒浓度的Rh2和低效浓度的顺铂联合作用于A549DDP细胞作为Rh2+DDP组，作用时间为48小时，以不加药物干预作为对照组，Hoechst33258染色荧光显微镜下观察细胞形态的变化，流式细胞仪检测细胞凋亡峰；紫外光分光光度计检测线粒体PTP开放情况，流式细胞仪检测细胞内钙离子浓度及线粒体跨膜电位的变化，Westton blot检测线粒体内细胞色素C释放情况及Caspase-3的活性，γ计数仪检测细胞摄取〓Tc-MIBI放射性活性。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

"From the observational point of view, differential tomography with active sources or multiplets, dense arrays of continuous GPS receivers and of borehole strain meters and tilt meters, and deep borehole observations in fault zones (for tracking the role of fluids directly), might be the key to success."

&从可观察的角度，带活化源或多重线的差分X线断层摄影术、密集排列的连续的GPS〔全球卫星定位系统〕接受仪，和钻洞应变仪和地倾仪，以及进行断层区域深孔钻洞观察〔以直接跟踪流体的作用〕，可能是导致成功的关键。&

与观察仪相关的网络解释 [注：此内容来源于网络，仅供参考]

DNA synthesizer：合成仪

DNA测序仪 DNA Sequencers | DNA合成仪 DNA synthesizer | 紫外观察灯 Ultraviolet Lamp

DNA synthesizer：测序仪

多肽合成仪 Peptide synthesizer | DNA测序仪 DNA synthesizer | 紫外观察灯 Ultraviolet Lamp

range finder：测距仪

首先解释单镜头反光数码相机和具有测距仪的数码相机有何不同:具有测距仪(range finder)的数码相机,其测距是以影像的平均清晰度为准的,单镜头反光摄影面则是通过取景器观察影像的清晰度来调节的,而这种调节是不同层面的清晰度对比来决定的,

Hybridization Oven：紫外观察灯 Ultraviolet Lamp 分子杂交仪

DNA合成仪 DNA synthesizer | 紫外观察灯 Ultraviolet Lamp 分子杂交仪 Hybridization Oven | PCR仪 PCR Amplifier

strain tensor：胁变张量,应变张量

straightness of line of sight 瞄准线,平直度 | strain tensor 胁变张量,应变张量 | strain viewer 胁变观察仪,应变观察仪

Ultraviolet Lamp：紫外观察灯

DNA合成仪 DNA synthesizer | 紫外观察灯 Ultraviolet Lamp | 分子杂交仪 Hybridization Oven

visualizer：观察仪

n-维观察仪(Visualizer)允许数据在 n-维空间中交互式旋转,选择像元组进行分类,以及聚集类,使其它类的选择更容易. 选择的类可以输出到感兴趣区(ROIs) ,并用作分类、不混溶和匹配的滤波技术的输入. n-维Visualizer通常用于最小噪声部分(MNF)的数据,

visualizer：观察器,显影仪

visual field plotter 视野描绘器 | visualizer 观察器,显影仪 | visual prostatic electrotome 目视前列腺电刀

rotascope：转动机观察仪

rotarian 埘轮社的会员 | rotascope 转动机观察仪 | rotational 轮流的

white-light coronograph：白光日冕观察仪

white-leaved oak shales ==> 白叶栎页岩 | white-light coronograph ==> 白光日冕观察仪 | white-light fringe ==> 白光干涉条纹